A radioimmunoassay for thromboxane B2

Fitzpatrick, F. A.; Gorman, Robert R.; Guire, J.C. Mc; Kelly, Robert C.; Wynalda, Michael A.; Sun, Frank F.

doi:10.1016/0003-2697(77)90127-0

Cited by 119 publications

(21 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…They were incubated at 37°C for 45 min, and then at 20°C for 2 h. Then, the samples were centrifuged at 2,000 g for 10 min, and the serum was frozen and stored for analysis. Immunoreactive thromboxane B2 was measured by a previously described method (21).…”

Section: Methodsmentioning

confidence: 99%

Endogenous prostacyclin biosynthesis and platelet function during selective inhibition of thromboxane synthase in man.

FitzGerald¹,

Brash²,

Oates³

et al. 1983

J. Clin. Invest.

136

View full text Add to dashboard Cite

A B S T R A C T The consequences of inhibiting the metabolism of prostaglandin G2 to thromboxane A2 in man were studied by using an inhibitor of thromboxane synthase, 4-[2-(IH-imidazol-1-yl)ethoxy] benzoic acid hydrochloride (dazoxiben). Single doses of 25, 50, 100, and 200 mg of dazoxiben were administered to healthy volunteers at 2-wk intervals in a randomized, placebo-controlled, double-blind manner. Serum thromboxane B2 and aggregation studies in whole blood and platelet-rich plasma were measured before dosing and at 1, 4, 6, 8, and 24 h after dosing. Both serum thromboxane B2 and the platelet aggregation response to arachidonic acid (1.33 mM) were reversibly inhibited in a dose-dependent manner. Aggregation induced by 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (0.4 and 4.0 ,uM) in platelet-rich plasma as well as both aggregation and nucleotide release induced by collagen (95 Zg/ml) in platelet-rich plasma and whole blood were unaltered by dazoxiben. Additional evidence for a platelet-inhibitory effect of the compound was a significant prolongation of the bleeding time at 1 h after administration of the highest dose (200 mg) of dazoxiben. Endogenous prostacyclin biosynthesis was assessed by measurement of the major urinary metabolite of prostacyclin, 2,3-dinor-6-ketoPGFia (PGI-M). PGI-M excretion was increased by

show abstract

Section: Methodsmentioning

confidence: 99%

Endogenous prostacyclin biosynthesis and platelet function during selective inhibition of thromboxane synthase in man.

FitzGerald¹,

Brash²,

Oates³

et al. 1983

J. Clin. Invest.

136

View full text Add to dashboard Cite

show abstract

“…until RIA was performed. Samples were then analyzed in duplicate according to standard protocol (18,19). No matrix effects due to protein present in unknown piglet plasma samples were found when standard curves run with eicosanoid-free piglet plasma prepared by charcoal stripping were compared to curves generated from samples not treated with charcoal.…”

Section: Dazmegrel Preparation Dazmegrel (Ukmentioning

confidence: 99%

Tumor Necrosis Factor-Induced Neonatal Pulmonary Hypertension: Effects of Dazmegrel Pretreatment

et al. 1990

View full text Add to dashboard Cite

ABSTRACT. The endogenously produced cytokine, tumor necrosis factor-a (TNF-a), has been shown in adult animal models to be associated with many of the pathophysiologic effects of sepsis, including systemic hypotension and hemorrhagic necrosis. TNF-a can induce the release of various vasoactive arachidonic acid metabolites, suggesting that TNF-a may act either directly or via intermediary substances in producing its effects. The pathophysiologic role of TNF-a in neonatal sepsis, especially its potential effect on pulmonary vascular tone, is presently unknown. To assess the role of TNF-a in neonatal sepsis, 19 piglets (19

show abstract

“…Quantitation was accomplished by stable-isotope dilution with a Hewlett Packard 5980 instrument operated in the negative-ion mode, monitoring m/z 586 for endogenous, 2,3-dinor-6-keto-PGFI,, and m/z 590 for the deuterium-labeled internal standard. Thromboxane B, was determined by a modification of the method of Fitzpatrick et al 22 Statistical analysis. Multiple means were compared by twoway analysis of variance and paired data by Student's paired t test.23 A two-tailed probability of 5% or less was considered significant.…”

mentioning

confidence: 99%

The effects of organic nitrates on prostacyclin biosynthesis and platelet function in humans.

Fitzgerald¹,

Roy²,

Robertson³

et al. 1984

Circulation

View full text Add to dashboard Cite

The results of prior studies indicate that nitroglycerin stimulates prostacyclin release by cultured endothelium and by the coronary vasculature in vivo. However, the accuracy of these findings in coronary vasculature relies on plasma samples obtained from the circulation via cardiac catheters, a procedure we have shown to stimulate prostacyclin release, thereby confounding'interpretation of drug action. We studied the effects of short-acting (nitroglycerin) and long-acting (isosorbide dinitrate) nitrates on a noninvasive index of prostacyclin synthesis, excretion of urinary 2,3-dinor-6-keto-PGF,,. Nitroglycerin was infused into six subjects to either a maximum of 480 gg/min or until mean arterial pressure fell by 20 mm Hg. Urine was collected for negative ion chemical ionization gas chromatographic, mass spectrometric analysis before and during the nitroglycerin infusion and for two 2 hr periods after nitroglycerin. The 297-302, 1984. NITROGLYCERIN is a potent vasodilator that is widely used in 'the treatment of angina pectoris.' Despite its availability for over 100 years, the basis of its effect on vascular smooth muscle is poorly understood. Nitroglycerin also inhibits platelet function in vitro2 3 and has been reported to prolong the bleeding time in human beings.4 5 In view of these biological properties, it has been postulated that nitroglycerin mediates these effects by enhancing generation of the arachidonic acid metabolite prostacyclin in vivo. Formed From the Divisions of Clinical Pharmacology and Cardiology, Van-

show abstract

A radioimmunoassay for thromboxane B2

Cited by 119 publications

References 5 publications

Endogenous prostacyclin biosynthesis and platelet function during selective inhibition of thromboxane synthase in man.

Endogenous prostacyclin biosynthesis and platelet function during selective inhibition of thromboxane synthase in man.

Tumor Necrosis Factor-Induced Neonatal Pulmonary Hypertension: Effects of Dazmegrel Pretreatment

The effects of organic nitrates on prostacyclin biosynthesis and platelet function in humans.

Contact Info

Product

Resources

About