2008
DOI: 10.1186/1476-511x-7-47
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A rapid 3% polyacrylamide slab gel electrophoresis method for high through put screening of LDL phenotype

Abstract: BackgroundSmall dense LDL is reported to be associated with increased coronary artery disease risk by various epidemiological studies. The gold standard for separation and identification of LDL subtypes in plasma is ultracentrifugation which is a lengthy procedure and difficult to perform. Various other methods like NMR, HPLC, gradient gel electrophoresis (GGE) have been reported for LDL sub fractionation all of which require specialized equipments and expertise. We report here a high throughput 3% polyacrylam… Show more

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Cited by 15 publications
(12 citation statements)
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“…LDL particle size was determined in EDTA plasma by electrophoresis 16. Briefly, a 3% slab gel was prepared by mixing 3.7 ml of solution 1, 3.7 ml of solution 2, 0.1 ml of tetramethyl ethylenediamine (TEMED) and 0.5 ml of ammonium per sulphate (APS).…”
Section: Methodsmentioning
confidence: 99%
“…LDL particle size was determined in EDTA plasma by electrophoresis 16. Briefly, a 3% slab gel was prepared by mixing 3.7 ml of solution 1, 3.7 ml of solution 2, 0.1 ml of tetramethyl ethylenediamine (TEMED) and 0.5 ml of ammonium per sulphate (APS).…”
Section: Methodsmentioning
confidence: 99%
“…LDL particle size was categorised into three categories: A (. 26 (26) . We tested for associations between follow-up LDL particle size category and treatment groups using the Mantel -Haenzel mean score test statistic.…”
Section: Statistical Analysesmentioning
confidence: 99%
“…These submicron particles also offer the advantage of uniform dispersion in water, which facilitates the mass transfer and phase partitioning of E2 molecules in a short binding time 15. For the detection and characterization of MIP submicron particles, SEM 16, dynamic light scattering (DLS) 17, ultrasound spectroscopy 18, field flow fractionation 19, size exclusion chromatography 20, gel electrophoresis 21, capillary gel electrophoresis 22 and CE 23, 24 have previously been reported. CE for the analysis of colloidal or nano‐particles ( e.g.…”
Section: Introductionmentioning
confidence: 99%