A rapid algorithm for processing digital physiologic signals: Application to skeletal muscle contractions

Jayaraman, Roop Chand; Latourette, Matthew T.; Siebert, James E.; Wiseman, Robert W.

doi:10.1016/j.bspc.2006.12.002

Cited by 5 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analysis of mechanical transients was performed using a custom algorithm 29 in a MatLab programming environment (Mathworks, Natick, MA). In brief, raw digital signals are passed through a low-pass filter to smooth the data and then analyzed for force output and tension-time integrals.…”

Section: Methodsmentioning

confidence: 99%

“…Kinetic parameters for the rise time (time to peak force) and relaxation time (time of return to baseline) were also measured in individual muscle twitches as previously described. 29 Statistical analysis of data after 5 days of treatment was performed using two-way analysis of variance with genotype and hormone treatment as independent variables (SPSS Inc., Chicago, IL). Group differences were considered significant at p<0.05 and post hoc comparisons were done using the Tukey post-hoc test.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Androgen receptors in muscle fibers induce rapid loss of force but not mass: Implications for spinal bulbar muscular atrophy

et al. 2013

Self Cite

View full text Add to dashboard Cite

Introduction Testosterone (T) induces motor dysfunction in transgenic (Tg) mice overexpressing wild type androgen receptor (AR) in skeletal muscles. Since many genes implicated in motor neuron disease are expressed in skeletal muscles, mutant proteins may act in muscles to instigate muscle dysfunction in motor neuron disease. Methods We examined contractile properties of the extensor digitorum longus (EDL) and soleus (SOL) muscles in vitro after 5 and 3 days of T treatment in motor-impaired Tg female mice. Results Both muscles showed deficits in tetanic force after 5 days of T treatment, without losses in muscle mass, protein content, or fiber number. By 3 days of T treatment, only SOL showed a deficit in tetanic force comparable to that at 5 days of treatment. In both treatments, EDL shows slowed twitch kinetics, whereas SOL shows deficits in the twitch/tetanus ratio. Conclusions These results suggest calcium handling mechanisms in muscle fibers are defective in motor-impaired mice.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Androgen receptors in muscle fibers induce rapid loss of force but not mass: Implications for spinal bulbar muscular atrophy

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Supramaximal stimulation was applied at a rate of 10 Hz for 4 h (42,63,73). Force was digitally captured using an AT MIO 16E analog-to-digital board and software (National Instruments, Austin, TX) and analyzed using custom MatLab software (MathWorks, Natick, MA) (39). After cessation of the stimulation period, test twitches were elicited via the sciatic nerve to assess nerve viability and to confirm recover of muscle force in each animal before tissue harvest.…”

Section: Methodsmentioning

confidence: 99%

Acute molecular response of mouse hindlimb muscles to chronic stimulation

LaFramboise

Jayaraman

Bombach

et al. 2009

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

Stimulation of the mouse hindlimb via the sciatic nerve was performed for a 4-h period to investigate acute muscle gene activation in a model of muscle phenotype conversion. Initial force production (1.6 +/- 0.1 g/g body wt) declined 45% within 10 min and was maintained for the remainder of the experiment. Force returned to initial levels upon study completion. An immediate-early growth response was present in the extensor digitorum longus (EDL) muscle (FOS, JUN, activating transcription factor 3, and musculoaponeurotic fibrosarcoma oncogene) with a similar but attenuated pattern in the soleus muscle. Transcript profiles showed decreased fast fiber-specific mRNA (myosin heavy chains 2A and 2B, fast troponins T(3) and I, alpha-tropomyosin, muscle creatine kinase, and parvalbumin) and increased slow transcripts (myosin heavy chain-1beta/slow, troponin C slow, and tropomyosin 3y) in the EDL versus soleus muscles. Histological analysis of the EDL revealed glycogen depletion without inflammatory cell infiltration in stimulated versus control muscles, whereas ultrastructural analysis showed no evidence of myofiber damage after stimulation. Multiple fiber type-specific transcription factors (tea domain family member 1, nuclear factor of activated T cells 1, peroxisome proliferator-activated receptor-gamma coactivator-1alpha and -beta, circadian locomotor output cycles kaput, and hypoxia-inducible factor-1alpha) increased in the EDL along with transcription factors characteristic of embryogenesis (Kruppel-like factor 4; SRY box containing 17; transcription factor 15; PBX/knotted 1 homeobox 1; and embryonic lethal, abnormal vision). No established in vivo satellite cell markers or genes activated in our parallel experiments of satellite cell proliferation in vitro (cyclins A(2), B(2), C, and E(1) and MyoD) were differentially increased in the stimulated muscles. These results indicated that the molecular onset of fast to slow phenotype conversion occurred in the EDL within 4 h of stimulation without injury or satellite cell recruitment. This conversion was associated with the expression of phenotype-specific transcription factors from resident fiber myonuclei, including the activation of nascent developmental transcriptional programs.

show abstract

“…The pulse duration was 0.2 ms and stimulation trains for tetanic force were delivered at fusion frequency (~70 Hz) for 0.5-1.2 s. Force was recorded using an ADC model AT MIO16E (National Instruments, Austin, TX, USA) controlled by commercially available software (LabScribeNI, iWorx, Dover, NH, USA). Analysis of mechanical transients was performed using a custom algorithm for physiological data developed in this laboratory using the MATLAB programming environment (MathWorks, Natick, MA, USA) [112].…”

Section: Studies On Intact Muscles From Micementioning

confidence: 99%

Creatine Kinase Equilibration and ΔGATP over an Extended Range of Physiological Conditions: Implications for Cellular Energetics, Signaling, and Muscle Performance

Wiseman,

Brown,

Beck

et al. 2023

IJMS

View full text Add to dashboard Cite

In this report, we establish a straightforward method for estimating the equilibrium constant for the creatine kinase reaction (CK Keq″) over wide but physiologically and experimentally relevant ranges of pH, Mg2+ and temperature. Our empirical formula for CK Keq″ is based on experimental measurements. It can be used to estimate [ADP] when [ADP] is below the resolution of experimental measurements, a typical situation because [ADP] is on the order of micromolar concentrations in living cells and may be much lower in many in vitro experiments. Accurate prediction of [ADP] is essential for in vivo studies of cellular energetics and metabolism and for in vitro studies of ATP-dependent enzyme function under near-physiological conditions. With [ADP], we were able to obtain improved estimates of ΔGATP, necessitating the reinvestigation of previously reported ADP- and ΔGATP-dependent processes. Application to actomyosin force generation in muscle provides support for the hypothesis that, when [Pi] varies and pH is not altered, the maximum Ca2+-activated isometric force depends on ΔGATP in both living and permeabilized muscle preparations. Further analysis of the pH studies introduces a novel hypothesis around the role of submicromolar ADP in force generation.

show abstract

A rapid algorithm for processing digital physiologic signals: Application to skeletal muscle contractions

Cited by 5 publications

References 31 publications

Androgen receptors in muscle fibers induce rapid loss of force but not mass: Implications for spinal bulbar muscular atrophy

Androgen receptors in muscle fibers induce rapid loss of force but not mass: Implications for spinal bulbar muscular atrophy

Acute molecular response of mouse hindlimb muscles to chronic stimulation

Creatine Kinase Equilibration and ΔGATP over an Extended Range of Physiological Conditions: Implications for Cellular Energetics, Signaling, and Muscle Performance

Contact Info

Product

Resources

About