A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) method and validation for progestogen multi-residues in feed

Abstract

Some progestogens as anabolic steroids can be illegally used for increasing the effi ciency of animal food production. The aim of the study was to develop convenient screening method against progestogens in feed. A sensitive enzyme-linked immunosorbent assay (ELISA) method was developed and optimized. The ELISA method is class-specifi c for four relative progestogens and the IC 50 of the method was 3.0 μg/l for MPA. A simple feed pre-treatment method was developed for analysis progestogen residues in feed. The limits of detection (LOD) were 2.0 μg/kg for MPA, 5.0 μg/kg for CMA, 4.6 μg/kg for MEGA and 3.9 μg/kg for HPA in the feed. The recovery was between 72.1 and 95.4% and the coeffi cients of variation (CV) were 7.2 to 15.8%. The results have showed the simple sample pre-treatment method was suitable for progestogen multi-residues rapid analysis in feed.

Ultrasensitive Nano‐ELISA for Detecting Sulfadimethoxine in Chicken Tissue

“…Although high sensitivity has been achieved in the above-mentioned immunoassays, accomplishing the detection of veterinary drug residue in animal tissues simply and sensitively by ELISA method is still challenging due to serious matrix interference [12,13]. Nevertheless, developing highly sensitive ELISA method can avoid this problem by diluting the sample extract and decreasing the matrix interference obviously [12,[14][15][16].…”