Some progestogens as anabolic steroids can be illegally used for increasing the effi ciency of animal food production. The aim of the study was to develop convenient screening method against progestogens in feed. A sensitive enzyme-linked immunosorbent assay (ELISA) method was developed and optimized. The ELISA method is class-specifi c for four relative progestogens and the IC 50 of the method was 3.0 μg/l for MPA. A simple feed pre-treatment method was developed for analysis progestogen residues in feed. The limits of detection (LOD) were 2.0 μg/kg for MPA, 5.0 μg/kg for CMA, 4.6 μg/kg for MEGA and 3.9 μg/kg for HPA in the feed. The recovery was between 72.1 and 95.4% and the coeffi cients of variation (CV) were 7.2 to 15.8%. The results have showed the simple sample pre-treatment method was suitable for progestogen multi-residues rapid analysis in feed.