A rapid and sensitive LC/MS/MS assay for the quantitation of brimonidine in ocular fluids and tissues

Jiang, Sherwin; Chappa, Arvind K.; Proksch, Joel W.

doi:10.1016/j.jchromb.2008.11.009

Cited by 35 publications

(14 citation statements)

References 8 publications

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“…This correlates with low concentration levels in biological fluids, demanding the use of very sensitive methods. Therefore, a couple of the hyphenated techniques like gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) were reported [4,5], aiming to BRIM detection and determination in human plasma, blood serum and ocular fluids. Tzovolou et al proposed capillary electrophoretic (CE) analysis of BRIM in the aqueous humor of the eye and blood serum [6].…”

Section: Introductionmentioning

confidence: 99%

An extensive study of electrochemical behavior of brimonidine and its determination at glassy carbon electrode

Aleksic

Radulović

Agbaba

et al. 2013

Electrochimica Acta

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Section: Introductionmentioning

confidence: 99%

An extensive study of electrochemical behavior of brimonidine and its determination at glassy carbon electrode

Aleksic

Radulović

Agbaba

et al. 2013

Electrochimica Acta

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“…An extensive literature survey revealed a gas chromatographic‐mass spectrometric (GC‐MS) assay for analysis of brimonidine tartarate in human plasma 3. A literature survey reveals few high‐performance liquid chromatography, (HPLC) 4–6, liquid chromatography‐mass spectrometry (LC‐MS) 7 methods reported for the estimation of brimonidine tartarate in blood serum and in ocular fluids. A stability indicating assay method using hydrophilic interaction liquid chromatography (HILIC) has also been reported 8.…”

Section: Introductionmentioning

confidence: 99%

An Electroanalytical Approach to Brimonidine at Boron Doped Diamond Electrode Based on Its Extensive Voltammetric Study

et al. 2012

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An extensive electrochemical study of brimonidine at boron doped diamond electrode (BDDE) was done by applying cyclic voltammetry (CV) and square‐wave voltammetry (SWV) in sulfuric acid of different concentrations (pH ranged from 0.6 to 1.6), and in BR buffer (pH ranged from 2.0 to 9.0). It was found that the reduction of brimonidine occurred in a one‐step quasi‐reversible mechanism, involving the transfer of two electrons and two protons. The reduction process took place at the quinoxaline ring and the corresponding mechanism of reduction was confirmed to be the same as for the other quinoxaline derivatives. The nature of the electrode process was found to be diffusion controlled in acid medium, while in a more alkaline medium a certain degree of adsorption was noticed. Based on this study, two sensitive voltammetric methods, differential pulse (DPV) and square wave (SWV) were developed, fully validated and suggested for rapid electroanalytical determination of low concentrations of brimonidine. The linearity was achieved within the concentration range from 2×10−6 M to 3×10−5 M for DPV (LOD=6.31×10−7 M, LOQ=2.1×10−6 M) and from 5×10−7 M to 1.5×10−5 M for SWV (LOD=1.28×10−7 M, LOQ=4.28×10−7 M). The methods were applied for brimonidine determination in pharmaceutical dosage form, eye drops. The obtained good recoveries suggested these simple and accurate methods for quality control of brimonidine in dosage form. Due to its high sensitivity, the SWV method could be a good alternative for determination of low concentrations of brimonidine, even in biological samples.

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“…Literature review reveals that different methods have been reported for estimation of both drugs individually like, RP-HPLC for the Analysis of Brimonidine in Ophthalmic Formulations [2,3], in blood serum and aqueous humor of the eye [4] and LC/MS/MS HPLC for the Analysis of Brimonidine in Ocular Fluids and Tissues [5]. Three papers discussed stability of Brimonidine tartrate [6][7][8], other analytical techniques were reported for determination of Brimonidine tartrate like HPTLC [9], GC/MS [10] and CE [11].…”

Section: Timolol Maleate Is (2s)mentioning

confidence: 99%

Development and Validation of HPLC Method for Simultaneous Estimation of Brimonidine Tartrate and Timolol Maleate in Bulk and Pharmaceutical Dosage Form

Elshanawane¹,

Abdel‐Aziz²,

Mohram³

et al. 2014

J Chromat Separation Techniq

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Brimonidine tartrate and Timolol Maleate are used in treatment of glaucoma by decreasing intra ocular pressure. A validated HPLC method was developed for the assay of them. The method was performed on BDS HYPERSIL Cyano column (250×4.6 mm, 5 μ) and the mobile phase consisted of Ammonium acetate (pH 5.0, 0.01 M) -Methanol (40:60, V/V) which pumped at a flow rate equals to 1.5 ml/min at ambient temperature. 20 µl of drugs sample solutions were monitored at two fixed wavelengths (lambda=254.0 nm for Brimonidine Tartrate and 300.0 nm for Timolol Maleate). The proposed method was validated in terms of linearity, accuracy, precision and limits of detection and quantitation according to ICH.

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A rapid and sensitive LC/MS/MS assay for the quantitation of brimonidine in ocular fluids and tissues

Cited by 35 publications

References 8 publications

An extensive study of electrochemical behavior of brimonidine and its determination at glassy carbon electrode

An extensive study of electrochemical behavior of brimonidine and its determination at glassy carbon electrode

An Electroanalytical Approach to Brimonidine at Boron Doped Diamond Electrode Based on Its Extensive Voltammetric Study

Development and Validation of HPLC Method for Simultaneous Estimation of Brimonidine Tartrate and Timolol Maleate in Bulk and Pharmaceutical Dosage Form

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