A rapid and sensitive method for the determination of ascorbic acid using 4,7-diphenyl-1,10-phenanthroline.

Arakawa, Nobuhiko; Tsutsumi, Kimiko; Sanceda, Norlita G.; Kurata, Tadao; Inagaki, Choten

doi:10.1271/bbb1961.45.1289

Cited by 97 publications

(45 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The homogenate was filtered using four layers of miracloth and centrifuged at 16,000 × g for 10 min at 4°C. The supernatant was used for the ascorbic acid assay after the procedure described by Arakawa et al (1981). This assay is based on the reduction of ferric ion to ferrous ion with ascorbic acid in acid solution followed by the formation of red chelate between ferrous ion and 4,7-diphenyl-1,10-phenanthroline (bathophenanthroline) that absorbs at 534 nm.…”

Section: Determining the Effect Of Mj On Changes In Oxygen Scavengingmentioning

confidence: 99%

Methyl Jasmonate Reduces Water Stress in Strawberry

Wang

1999

J Plant Growth Regul

111

View full text Add to dashboard Cite

The effect of methyl jasmonate (MJ) on changes of oxygen-scavenging enzyme activities and membrane lipid composition was studied in strawberry leaves under water stress. Under water stress, MJ treatment reduced the increase of peroxidase (EC 1.11.1.7; POD) activity, maintained higher catalase (EC 1.11.1.6; CAT) and superoxide dismutase (EC 1.15.1.1; SOD) activities, and ascorbic acid content. In addition, MJ treatment reduced transpiration and membrane-lipid peroxidation as expressed by malondialdehyde (MDA) content, lessened the reduction of membrane lipids, glycolipids [monogalactosyl diglyceride (MGDG), digalactosyl diglyceride (DGDG)], and phospholipids [phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and phosphatidylinositol (PI)]. In water-deficit conditions, MJ treatment also alleviated the decline in the degree of fatty acid unsaturation and the ratio of linolenic (18:3) to linoleic acid (18:2). These results indicate that MJ treatment appears to alter the metabolism of strawberry plants rendering the tissue better able to withstand water stress.

show abstract

Section: Determining the Effect Of Mj On Changes In Oxygen Scavengingmentioning

confidence: 99%

Methyl Jasmonate Reduces Water Stress in Strawberry

Wang

1999

J Plant Growth Regul

111

View full text Add to dashboard Cite

show abstract

“…Ascorbic acid is an essential component of plant tissues and has been the focus of numerous studies regarding the enzymatic and non-enzymatic oxidation in biological systems. It serves as an excellent antioxidant and plays a key role in the H 2 O 2 removal by the ascorbate/glutathione cycle and produces DHA (Arakawa et al, 1981). ROS are involved in the oxidation of ascorbic acid to form dehydroascorbate, which is subsequently regenerated to ascorbic acid again.…”

Section: Resultsmentioning

confidence: 99%

“…The concentration of the complex MDA/TBARS was calculated using the extinction coefficient of 1.55 mmol L -1 cm -1 . Ascorbate (Asc) and dehydroascorbate (DHA) were quantified as described by Arakawa et al (1981) with some modifications. One hundred milligrams of leaf tissue were macerated in 2 mL of trichloroacetic acid (TCA) 5% (w/v) homogenate and centrifuged at 10,000 × g for 15 min at 4ºC.…”

Section: Methodsmentioning

confidence: 99%

Stomatal behavior and components of the antioxidative system in coffee plants under water stress

Deuner¹,

Alves²,

Zanandrea

et al. 2011

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

Coffee (Coffea arabica) plants show a positive relationship between stomatal closure and formation and accumulation of H 2 O 2 . However, for coffee plants under water restriction such relationship has never been studied. The objective of the present study was evaluate the stomatal movement and the antioxidant capacity of coffee seedlings under different water regimes. Eight months old coffee seedlings of cv. Catuaí IAC 99 were submitted to field capacity, gradual and total suspension of irrigation during a period of 21 days. Evaluations of leaf water potential (Ψ w ) were performed in the beginning of the morning, and stomatal resistance, transpiration rate and vapor pressure deficit were determined at 10 am and 5 pm. All biochemical and enzymatic determinations were performed in leaves collected at 5 pm. Evaluations and samplings were performed at three days intervals. There was no variation in Ψ w during the evaluated period for plants in field capacity. However, an expressive decrease of Ψ w following day 12, reaching values near -2.5 MPa at the end of the experiment was observed for plants submitted to gradual suspension of irrigation. For plants submitted to total suspension of irrigation, Ψ w decreases after the sixth day, reaching -2.5 MPa at day 15. The decay of Ψ w in plants submitted to gradual and total suspension of irrigation reflected in increased stomatal resistance and in a decreased transpiration rate leading to an increase in hydrogen peroxide formation and, on final stages, increase in lipid peroxidation. As a conclusion, an increase in the activity of antioxidant enzymes as well as in the levels of ascorbate and dehydroascorbate was observed, which act in the detoxification of free radicals formed as result of the water stress. Key words: Coffea arabica, oxidative stress, antioxidants, water stressComportamento estomático e componentes do sistema antioxidante em cafeeiros sob estresse hídrico RESUMO: Para o cafeeiro (Coffea arabica) existe uma comprovada relação positiva entre fechamento estomático e formação e acúmulo de H 2 O 2 . Entretanto, tal relação para a cultura sob restrição hídrica ainda não foi estudada. Avaliou-se o movimento estomático e a capacidade antioxidante em mudas de cafeeiro sob diferentes regimes hídricos. Mudas de cafeeiro cv. Catuaí IAC 99, com oito meses de idade, foram submetidas à capacidade de campo, suspensão gradativa e suspensão total da irrigação por um período de 21 dias. Foram realizadas avaliações do potencial hídrico (Ψ w ) foliar na antemanhã e resistência estomática, taxa transpiratória e déficit de pressão de vapor foram avaliados as 10h00 e 17h00. As determinações bioquímicas e enzimáticas foram realizadas em folhas coletadas às 17h00. Todas as avaliações e coletas foram realizadas em intervalos de três dias. Nas plantas em capacidade de campo não houve variação no Ψ w durante o período de avaliação. Para a suspensão gradativa da irrigação, houve queda expressiva a partir dos 12 dias, chegando próximo a -2,5 Mpa, ao final do experimento. Já ...

show abstract

“…Ascorbic acid (AA) contents were determined as described by Arakawa et al (1981). Briefly, 1 g of coriander sample was extracted in 5.0 mL of 5% trichloroacetic acid (TCA), and then 1.0 mL of the sample extract solution was added with 1.0 mL of 5% TCA, 1.0 mL of ethyl alcohol (EtOH), 0.5 mL of 0.4% H 3 PO 4 -EtOH, l.0 mL of 0.5% Bathophenanthroline (BP)-EtOH, and finally 0.5 mL of 0.03% FeCl 3 -EtOH.…”

Section: Analysis Of Ascorbic Acidmentioning

confidence: 99%