A Rapid and Sensitive Method for the Determination of Ascorbic Acid Using Iron(III)-Ferronate Complex

Arya, Sunil; Mahajan, Meenakshi

doi:10.2116/analsci.11.853

Cited by 12 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spectrophotometry (22)(23)(24)(25)(26)(27)(28)(29)(30)(31), differential pulse polarography (32), and adsorption potentiometry (33) have been used to determine both AA and DHA (upon reduction to AA) in fruit juices and other industrial products. However, these methods lack the adequate selectivity to analyze vascular and nonvascular plant extracts (22,23).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination ofl-Ascorbic Acid, Glutathione, and Their Oxidized Forms in Ozone-Exposed Vascular Plants by Capillary Zone Electrophoresis

Herrero‐Martínez¹,

Simó‐Alfonso²,

Ramis‐Ramos³

et al. 2000

Environ. Sci. Technol.

View full text Add to dashboard Cite

Simultaneous determination of ascorbic acid (AA), glutathione (GSH), and oxidized glutathione (GSSG) in vascular plants has been achieved by capillary zone electrophoresis. A background electrolyte containing a phosphate buffer of pH 7 and hexadimethrin bromide for dynamic control of electroosmotic flow was used. Separation was performed in less than 5 min. The signal was simultaneously monitored at 265 and 200 nm. Dehydroascorbic acid (DHA) was determined in another aliquot of the same plant extract upon reduction with dl-homocysteine. Limits of detection of AA, GSH, and GSSG ranged from 0.2 to 0.8 μg mL-1, which corresponded from 0.04 to 0.16 mg per 100 g fresh weight of tomato, watermelon, and potato leaves. Plants exposed to a high ozone concentration (1600 μg m-3) in a laboratory fumigation system during 1, 2, 3, and 5 h showed progressive ozone-induced oxidative stress. This could be seen as a simultaneous decrease of the AA/(AA+DHA) and GSH/(GSH+GSSG) ratios; however, the decline rate was higher for AA/(AA+DHA) than for GSH/(GSH+GSSG) in plants cultivated in the field, in open-top chambers, during ca. 50 days with much lower ozone concentrations (mean concentrations, 10 and 80 μg m-3).

show abstract

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination ofl-Ascorbic Acid, Glutathione, and Their Oxidized Forms in Ozone-Exposed Vascular Plants by Capillary Zone Electrophoresis

Herrero‐Martínez¹,

Simó‐Alfonso²,

Ramis‐Ramos³

et al. 2000

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…Common additives of pharmaceutical preparations have no adverse effect on the absorbance of the complex. Another fast and facile method based on the proportionate decrease in absorbance of iron(III)-ferronate complex 127 (λ max =465 nm) by the addition of ascorbic acid was proposed by the same authors after extracting the complex into TBA/CHCl 3 solution. Beer's law is valid up to 10 µg ml -1 .…”

Section: Resultsmentioning

confidence: 99%

Photometric Methods for the Determination of Vitamin C

1998

Self Cite

View full text Add to dashboard Cite

“…The measured concentration of the unknown solutions are used to calculate the vitamin C degraded in solution over time. To achieve our goal, we reviewed a variety of spectrophotometric techniques available for the determination of vitamin C, including the reaction with 4-chloro-7-nitrobenzofurazane, 17 the iron(III)−ferronate complex formation, 18 the copper(II)− neocuproine reagent technique, 19 the oxidation of vitamin C with 2,6-dichlorophenollindophenol, 20 the FRAP assay method (reduction of ferric tripryidyltriazine to the ferrous form), 21 and the reduction of iron(III) to iron(II) by vitamin C and the subsequent complex formation with 1,10-phenanthroline (Figure 1). 22 The latter technique fit our needs the best, providing both stability, speed, and reproducibility.…”

Section: ■ Introductionmentioning

confidence: 99%

A Laboratory Experiment for Rapid Determination of the Stability of Vitamin C

et al. 2016

View full text Add to dashboard Cite

Experiments in laboratory manuals intended for general, organic, and biological (GOB) chemistry laboratories include few opportunities for students to engage in instrumental methods of analysis. Many of these students seek careers in modern health-related fields where experience in spectroscopic techniques would be beneficial. A simple, rapid, easily implemented experiment was developed to measure vitamin C levels in solutions using visible spectroscopy. When vitamin C is added to a mixed reagent containing Fe 3+ and 1,10-phenanthroline, the vitamin C reduces the Fe 3+ to Fe 2+ which subsequently combines with the 1,10-phenanthroline to form a reddish-orange colored complex that is measured by visible spectroscopy at 510 nm. The experiment includes measurement of the stability of aqueous vitamin C solutions when exposed to long-term storage and to heat. Students in GOB laboratory classes were able to produce results with good precision. The experiment was also extended to include the measurement of vitamin C in nutrition supplements.

show abstract

A Rapid and Sensitive Method for the Determination of Ascorbic Acid Using Iron(III)-Ferronate Complex

Cited by 12 publications

References 13 publications

Simultaneous Determination ofl-Ascorbic Acid, Glutathione, and Their Oxidized Forms in Ozone-Exposed Vascular Plants by Capillary Zone Electrophoresis

Simultaneous Determination ofl-Ascorbic Acid, Glutathione, and Their Oxidized Forms in Ozone-Exposed Vascular Plants by Capillary Zone Electrophoresis

Photometric Methods for the Determination of Vitamin C

A Laboratory Experiment for Rapid Determination of the Stability of Vitamin C

Contact Info

Product

Resources

About