A rapid and simple HPLC microassay for biogenic amines in discrete brain regions

Donzanti, Bruce A.; Yamamoto, Bryan K.

doi:10.1016/0091-3057(88)90102-5

Cited by 18 publications

(9 citation statements)

References 14 publications

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“…The relative concentration levels of monoamines and metabolites measured in the FCtx, STR, NAC, HIP, SNpc and VTA brain regions are presented in Table . These values accurately represent the degree of monoaminergic input within each brain region and are in agreement with the values previously reported for mice brain tissue (Donzanti & Yamamoto, ; Saito et al, ). Specifically, within the brain regions comprising the dopaminergic brain reward (NAc and VTA) and motor control (STR and SNpc) pathways, tissue levels of DA and respective metabolites were determined to be significantly higher than those of the FCtx and HIP, with the major DA projection sites, NAc and STR, containing the highest levels of DA (ng/mg) as compared with all other brain regions analyzed by this method.…”

Section: Resultssupporting

confidence: 91%

“…Of these previously developed HPLC‐ECD methodologies, measurements were achieved in larger brain regions, including the frontal cortex (FCtx), hippocampus (HIP), striatum (STR) and nucleus accumbens (NAc) but were not assessed in smaller regions, such as the dopaminergic SNpc and VTA, and LLOQs (signal‐to‐noise ratio ≥ 10) were not reported for these assays. In addition, these methodologies require ultrafiltration of prepared samples prior to analysis and are thus susceptible to analyte recovery issues (Bidel et al, ; Donzanti & Yamamoto, ; Saito, Murai, Abe, Masuda, & Itoh, ).…”

Section: Introductionmentioning

confidence: 99%

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A simple and sensitive high‐performance liquid chromatography–electrochemical detection assay for the quantitative determination of monoamines and respective metabolites in six discrete brain regions of mice

Allen

Rednour

Shepard

et al. 2017

Biomedical Chromatography

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A rapid, sensitive, and reproducible assay is described for the quantitative determination of the monoamine neurotransmitters dopamine, norepinephrine and serotonin, their metabolites, and the internal standard 3,4-dihydroxybenzlyamine hydro-bromide in mouse brain homogenate using high-performance liquid chromatography with electrochemical detection. The method was validated in the following brain areas: frontal cortex, striatum, nucleus accumbens, hippocampus, substantia nigra pars compacta and ventral tegmental area. Biogenic amines and relevant metabolites were extracted from discrete brain regions using a simple protein precipitation procedure, and the chromatography was achieved using a C column. The method was accurate over the linear range of 0.300-30 ng/mL (r = 0.999) for dopamine and 0.300-15 ng/mL (r = 0.999) for norepinephrine, 3,4-dihydroxybenzlyamine hydro-bromide, homovanillic acid and 5-hydroxyindolacetic acid, with detection limits of ~0.125 ng/mL (5 pg on column) for each of these analytes. Accuracy and linearity for serotonin were observed throughout the concentration range of 0.625-30 ng/mL (r = 0.998) with an analytical detection limit of ~0.300 ng/mL (12 pg on column). Relative recoveries for all analytes were approximately ≥90% and the analytical run time was <10 min. The described method utilized minimal sample preparation procedures and was optimized to provide the sensitivity limits required for simultaneous monoamine and metabolite analysis in small, discrete brain tissue samples.

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

A simple and sensitive high‐performance liquid chromatography–electrochemical detection assay for the quantitative determination of monoamines and respective metabolites in six discrete brain regions of mice

Allen

Rednour

Shepard

et al. 2017

Biomedical Chromatography

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“…In an additional experiment, dialysate samples (15 ml) were used to assay GABA. GLU and GABA were determined by high-performance liquid chromatography (HPLC) with fluorometric detection after pre-column derivatization with o-phthalaldehyde/b-mercaptoethanol (Sigma-Aldrich, Milan, Italy) reagent according to Donzanti and Yamamoto (1988). DA was determined by HPLC coupled to an electrochemical detector, as described elsewhere (Invernizzi et al, 1992).…”

Section: Neurochemical Studymentioning

confidence: 99%

Dopamine D1-Like and D2-Like Receptors in the Dorsal Striatum Control Different Aspects of Attentional Performance in the Five-Choice Serial Reaction Time Task Under a Condition of Increased Activity of Corticostriatal Inputs

Agnoli

Mainolfi

Invernizzi

et al. 2012

Neuropsychopharmacol

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We investigated the interaction between the corticostriatal glutamatergic afferents and dopamine D1-like and D2-like receptors in the dorsomedial striatum (dm-STR) in attention and executive response control in the five-choice serial reaction time (5-CSRT) task. The competitive NMDA receptor antagonist 3-(R)-2-carboxypiperazin-4-propyl-1-phosphonic acid (CPP) injected in the mPFC impaired accuracy and increased premature and perseverative responding, raising GLU, DA, and GABA release in the dm-STR. The D1-like antagonist SCH23390 injected in the dm-STR reversed the CPP-induced accuracy deficit but did not affect the increase in perseverative responding. In contrast, the D2-like antagonist haloperidol injected in the dm-STR reduced the CPP-induced increase in perseverative responding but not the accuracy deficit. The different roles of dorsal striatal D1-like and D2-like receptor were further supported by the finding that activation of D1-like receptor in the dm-STR by SKF38393 impaired accuracy but not perseverative responding while the D2-like agonist quinpirole injected in the dm-STR increased perseverative responding but did not affect accuracy. These findings suggest that integration of cortical information by D1-like receptors in the dm-STR is a key mechanism of the input selection process of attention while the integration of corticostriatal signals by D2-like receptors preserves the ability to switch from one act/response to the next in a complex motor sequence, thus providing for behavioral flexibility.

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“…The concentration of GABA in the dialysate samples was determined by HPLC with electrochemical detection as described previously by Donzanti and Yamamoto (1988). Briefly, GABA was assayed by precolumn derivatization with o-phthaldialdehyde.…”

Section: Data Analysis Analysis Of Behavioral Datamentioning

confidence: 99%

Role of External Pallidal Segment in Primate Parkinsonism: Comparison of the Effects of 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Parkinsonism and Lesions of the External Pallidal Segment

Soares¹,

Kliem²,

Betarbet³

et al. 2004

J. Neurosci.

179

185

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These experiments re-examined the notion that reduced activity in the external pallidal segment (GPe) results in the abnormalities of neuronal discharge in the subthalamic nucleus (STN) and the internal pallidal segment (GPi) and in the development of parkinsonian motor signs. Extracellular recording in two rhesus monkeys, which had been rendered parkinsonian by treatment with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), revealed that the average neuronal discharge rate decreased in GPe but increased in STN and GPi. After MPTP, neurons in all three nuclei tended to discharge in oscillatory bursts. In addition, GABA release in STN (measured with microdialysis) was reduced, indicative of reduced activity along the GPe-STN pathway. Finally, the concentration of glutamic acid dehydrogenase (GAD; measured with autoimmunoradiography) was increased in GPe and GPi, likely reflecting increased striatal input and increased activity of local axon collaterals, respectively. Surprisingly, GAD protein in STN remained unchanged, indicating that the usual assumption that GAD levels are determined primarily by the overall activity of GABAergic elements may be too simplistic. The results from the MPTP-treated animals were compared with results obtained in a second group of three animals with ibotenic acid lesions of GPe. GPe lesions resulted in increased discharge in STN and GPi, comparable with the changes seen after MPTP but did not induce oscillatory bursting and had no behavioral effects. The results indicate that a mere reduction of GPe activity does not produce parkinsonism. Other changes, such as altered discharge patterns in STN and GPi, may play an important role in the generation of parkinsonism.

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A rapid and simple HPLC microassay for biogenic amines in discrete brain regions

Cited by 18 publications

References 14 publications

A simple and sensitive high‐performance liquid chromatography–electrochemical detection assay for the quantitative determination of monoamines and respective metabolites in six discrete brain regions of mice

A simple and sensitive high‐performance liquid chromatography–electrochemical detection assay for the quantitative determination of monoamines and respective metabolites in six discrete brain regions of mice

Dopamine D1-Like and D2-Like Receptors in the Dorsal Striatum Control Different Aspects of Attentional Performance in the Five-Choice Serial Reaction Time Task Under a Condition of Increased Activity of Corticostriatal Inputs

Role of External Pallidal Segment in Primate Parkinsonism: Comparison of the Effects of 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Parkinsonism and Lesions of the External Pallidal Segment

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