A rapid bioluminescent method for the detection of methicillin-resistant <i>Staphylococcus aureus colonies</i>

Barton, A. P.

doi:10.1093/jac/15.1.61

Cited by 5 publications

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“…Mc Walter (1984), with a method similar to ours, tested 100 isolates with methicillin (at 30"C), gentamicin, Table I clindamycin, erythromycin and fusidic acid, and found good correlation with standard disk diffusion results and MIC values. Barton (1985) used a novel approach in assaying extracellular release from staphylococci after incubation with methicillin and treatment with lysostaphin ; although only 29 strains were examined, the results were similar to those with conventional tests. Unfortunately, this method is applicable only to S .…”

Section: Discussionmentioning

confidence: 93%

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A novel luminometer for rapid antimicrobial susceptibility tests on gram-positive cocci by ATP bioluminescence

Wheat

Spencer²,

Hastings³

1989

Journal of Medical Microbiology

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Summary. The susceptibility of 130 clinical isolates of gram-positive cocci to a wide range of antimicrobial agents was assessed by ATP bioluminescence in a 4-h test. ATP assays were performed on a novel luminometer, the Amerlite Analyser, which measures luminescence from microtitration trays. For most organisms tested, there was good correlation (>90%) with conventional MIC values estimated on 18-h cultures. However, a problem was found with detection of penicillin resistance in Staphylococcusaureus by the ATP method, 13% of strains showing major disagreement. Methicillin resistance of S. aureus was shown reliably for most strains (94%) by ATP assay, provided they were incubated at 30°C. The Amerlite Analyser offers the potential for the development of a semi-automated antimicrobial susceptibility test, with a significant reduction in reagent costs when compared with previously described bioluminescence protocols.

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Section: Discussionmentioning

confidence: 93%

“…Unfortunately, this method is applicable only to S . aureus, because other staphylococci may not be susceptible to lysostaphin (Barton, 1985). These studies and earlier reports on bioluminescence have relied on single-chamber luminometers (Picciolo and Chappelle, 1977 …”

Section: Discussionmentioning

confidence: 96%