A rapid fibroblast culture technique for high resolution karyotypes

Stanyon, Roscoe; Galleni, Lodovico

doi:10.1080/11250009109355732

Cited by 59 publications

(41 citation statements)

References 9 publications

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“…Primary fibroblast cell lines of 2 females (1F, embryo; 2F, mother) and 1 male (3M) of E. robustus were established from different tissues using standard techniques, as described by Stanyon and Galleni [1991]. The animals were caught by hunters of the indigenous population of Chukotka Autonomous Okrug (Mechigmen bay of the Bering Sea, Lorino) who have permission to hunt this species for food.…”

Section: Methodsmentioning

confidence: 99%

Comparative Chromosome Map and Heterochromatin Features of the Gray Whale Karyotype (Cetacea)

Kulemzina

Proskuryakova

Beklemisheva

et al. 2016

Cytogenet Genome Res

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Cetacean karyotypes possess exceptionally stable diploid numbers and highly conserved chromosomes. To date, only toothed whales (Odontoceti) have been analyzed by comparative chromosome painting. Here, we studied the karyotype of a representative of baleen whales, the gray whale (Eschrichtius robustus, Mysticeti), by Zoo-FISH with dromedary camel and human chromosome-specific probes. We confirmed a high degree of karyotype conservation and found an identical order of syntenic segments in both branches of cetaceans. Yet, whale chromosomes harbor variable heterochromatic regions constituting up to a third of the genome due to the presence of several types of repeats. To investigate the cause of this variability, several classes of repeated DNA sequences were mapped onto chromosomes of whale species from both Mysticeti and Odontoceti. We uncovered extensive intrapopulation variability in the size of heterochromatic blocks present in homologous chromosomes among 3 individuals of the gray whale by 2-step differential chromosome staining. We show that some of the heteromorphisms observed in the gray whale karyotype are due to distinct amplification of a complex of common cetacean repeat and heavy satellite repeat on homologous autosomes. Furthermore, we demonstrate localization of the telomeric repeat in the heterochromatin of both gray and pilot whale (Globicephala melas, Odontoceti). Heterochromatic blocks in the pilot whale represent a composite of telomeric and common repeats, while heavy satellite repeat is lacking in the toothed whale consistent with previous studies.

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Section: Methodsmentioning

confidence: 99%

Comparative Chromosome Map and Heterochromatin Features of the Gray Whale Karyotype (Cetacea)

Kulemzina

Proskuryakova

Beklemisheva

et al. 2016

Cytogenet Genome Res

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“…The samples were then shipped by express courier to Italy or Germany where they were cultured. Stanyon & Galleni (1991).…”

Section: Methodsmentioning

confidence: 99%

A standardized G‐banded karyotype for the raccoon (Procyon lotor)compared with the domestic cat

Stanyon

Bigoni

Weinberg

et al. 1993

Bolletino di zoologia

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We propose a standardized karyotype for the raccoon (Procyon lotor; 2n = 38, FN 74) and compare it with that of the domestic cat (2n = 38, FN 72). Numerous chromosomes (12) have similar and sometimes identical G-banding and 14 chromosome pairs have remained intact. Other chromosomes apparently differ by Robertsonian translocations and inversions. The conservation of these karyotypes is remarkable considering that the divergence of procyonids and felids predates 50 million years B.P. However, the common diploid number of 38 is not a primitive retention, as sometimes hypothesized. Instead, cats and raccoons converged on this chromosome number by a different series of translocations. The unsolved problems of carnivore phylogeny could benefit from a combination of high resolution banding and molecular cytogenetic analyses.

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“…Cells were fixed for 30 min at −20°C and pelleted at 150 g at + 4°C. When supernatant was removed, cold fixative was added to obtain the desired concentration of nuclei (Stanyon and Galleni, 1991). About 10-15 μl of fixed material was air-dried on a clean degreased damp glass slide.…”

Section: Preparation Of Bmc Nuclei For Fish Analysismentioning

confidence: 99%

“Delayed death” phenomenon: A synergistic action of cyclophosphamide and exogenous DNA

Долгова¹,

Proskurina²,

Николин³

et al. 2012

Gene

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A rapid fibroblast culture technique for high resolution karyotypes

Cited by 59 publications

References 9 publications

Comparative Chromosome Map and Heterochromatin Features of the Gray Whale Karyotype (Cetacea)

Comparative Chromosome Map and Heterochromatin Features of the Gray Whale Karyotype (Cetacea)

A standardized G‐banded karyotype for the raccoon (Procyon lotor)compared with the domestic cat

“Delayed death” phenomenon: A synergistic action of cyclophosphamide and exogenous DNA

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