A rapid fluorescence “switch-on” assay for glutathione detection by using carbon dots–MnO2 nanocomposites

Cai, Qiaoling; Li, Jie; Ge, Jia; Zhang, Lin; Hu, Yingbin; Li, Zhaohui; Qu, Lingbo

doi:10.1016/j.bios.2015.04.077

Cited by 321 publications

(109 citation statements)

References 39 publications

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“…In the FT-IR analysis of N-CDs, the following were observed: stretching vibration of O–H at 3408 cm −1 and C–H at 2930 cm −1 , stretching vibration and bending vibration of N–H at 3256 cm −1 and 1586 cm −1 , bending vibration of the C–O at 1401 cm −1 and the stretching peak of C–O–C at 1111 cm −1 , C–N stretching vibration at 1246 cm −1 (Fig. 1d) 43–45 . These results indicate that there are abundant of hydroxyl, amino and carboxyl group on the surface of the N-CDs.…”

Section: Resultsmentioning

confidence: 94%

Nitrogen-doped Carbon Dots Mediated Fluorescent on-off Assay for Rapid and Highly Sensitive Pyrophosphate and Alkaline Phosphatase Detection

Geng

Zhang

et al. 2017

Sci Rep

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In this report, a novel fluorescent sensing platform using nitrogen-doped carbon dots (N-CDs) as probes for fluorescence signal transmission has been designed for the detection of significant biomolecules pyrophosphate (PPi) and alkaline phosphatase (ALP). The high fluorescent N-CDs could be selectively quenched by Cu2+, and recovered by the addition of PPi because PPi preferentially binds to Cu2+. Once ALP was introduced into the system, ALP can specifically hydrolyze PPi into Pi, the intense fluorescence of N-CDs could be quenched again due to the recombination of the as-released Cu2+ with N-CDs. So, fluorescence of N-CDs is regulated by an ALP-triggered reaction. Based on this strategy, we demonstrated that N-CDs could serve as a very effective fluorescent sensing platform for label-free, sensitive and selective detection of PPi and ALP with low detection limit of 0.16 μM and 0.4 U/L for PPi and ALP, respectively. Moreover, the assay time is just around 0.5 min for PPi and 30 min for ALP. This developed strategy shows remarkable advantages including sensitive, rapid, simple, convenient, and low-cost and so forth. Furthermore, this method was also successfully applied to monitor ALP in human serum, which indicates its great potential for practical applications in biological and clinical diagnosis.

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Section: Resultsmentioning

confidence: 94%

Nitrogen-doped Carbon Dots Mediated Fluorescent on-off Assay for Rapid and Highly Sensitive Pyrophosphate and Alkaline Phosphatase Detection

Geng

Zhang

et al. 2017

Sci Rep

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“…They have been, therefore, widely appliedf or developing fluorophores for biomedical applications. [8][9][10][11] One of the simplest routes to synthesise fluorescent C-dots from citric acid is thermal decomposition;d uring the treatment,C Ai sd ehydrated and reducedi nt he 180-200 8Ct emperaturer ange to obtain af luorescent material. The preparation of C-dotsf rom CA is, however,v ery sensitive to the synthesis conditions, such as decomposition temperature, time of thermal treatment [12] and use of solvent.…”

Section: Introductionmentioning

confidence: 99%

Carbon Dots from Citric Acid and its Intermediates Formed by Thermal Decomposition

Ludmerczki

Mura

Carbonaro

et al. 2019

Chemistry A European J

119

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Thermal decomposition of citric acid is one of the most common synthesis methods for fluorescent carbon dots; the reaction pathway is, however, quite complex and the details are still far from being understood. For instance, several intermediates form during the process and they also give rise to fluorescent species. In the present work, the formation of fluorescent C‐dots from citric acid has been studied as a function of reaction time by coupling infrared analysis, X‐ray photoelectron spectroscopy, liquid chromatography/mass spectroscopy (LC/MS) with the change of the optical properties, absorption and emission. The reaction intermediates, which have been identified at different stages, produce two main emissive species, in the green and blue, as also indicated by the decay time analysis. C‐dots formed from the intermediates have also been synthesised by thermal decomposition, which gave an emission maximum around 450 nm. The citric acid C‐dots in water show short temporal stability, but their functionalisation with 3‐aminopropyltriethoxysilane reduces the quenching. The understanding of the citric acid thermal decomposition reaction is expected to improve the control and reproducibility of C‐dots synthesis.

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“…In this sense, CDs have been applied for detection of biological (Cai et al 2015, Mehta et al 2015, Nandi et al 2015, Niu et al 2015, organic Huang et al 2014a, and inorganic (Yin et al 2013, Liu et al 2014b, Zhang and Chen 2014c, Zhao et al 2014a, Cui et al 2015, Gogoi et al 2015 species. Regarding inorganic trace analysis, a dramatic increase in the number of publications describing CD-based systems has occurred in the last 2 years (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Luminescent assays based on carbon dots for inorganic trace analysis

Costas-Mora

Romero

Lavilla

et al. 2015

Reviews in Analytical Chemistry

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Carbon dots (CDs) are a recently discovered class of fluorescent nanomaterials with great potential to be applied in the analytical field. CDs have demonstrated to be a promising alternative to conventional organic fluorophores or quantum dots as optical nanoprobes for sensing different chemical species. In this overview, we review the progress in the design of novel nanoprobes based on fluorescent CDs for inorganic trace analysis. Representative examples of CD-based assays are described and the different sensing strategies are discussed.

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A rapid fluorescence “switch-on” assay for glutathione detection by using carbon dots–MnO2 nanocomposites

Cited by 321 publications

References 39 publications

Nitrogen-doped Carbon Dots Mediated Fluorescent on-off Assay for Rapid and Highly Sensitive Pyrophosphate and Alkaline Phosphatase Detection

Nitrogen-doped Carbon Dots Mediated Fluorescent on-off Assay for Rapid and Highly Sensitive Pyrophosphate and Alkaline Phosphatase Detection

Carbon Dots from Citric Acid and its Intermediates Formed by Thermal Decomposition

Luminescent assays based on carbon dots for inorganic trace analysis

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