A rapid gas chromatographic method for the determination of BHA and BHT in vegetable oils

Abstract

A gas liquid chromatographic (GLC) technique has been developed which requires about 20 min for the determination of BHA and BHT in vegetable oils. This method involves the addition of an internal standard to a weighed portion of the oil, dilution of the mixture with carbon disulfide, and injection into the Gas Chromatograph. BHA and BHT are isolated from the nonvolatile vegetable oil by using a short precolumn located in the sample port block of the gas chromatograph. Up to 35 consecutive sample injections per day have been made on the same precolumn with no appreciable effect on the accuracy of the determination. The precolumn is cleaned at the end of each day's operation. The clean precoluran is allowed to equilibrate to sample port block temperature overnight for the following day's analysis. Identification of BHA and BHT can be confirmed with a second GLC column which reverses the elution order of these compounds. Soybean, cottonseed, corn and peanut oils fortified with 20, 60 and 100 ppm each of BHA and BHT showed a recovery range of 97% to 104%.

High-selective and sensitive voltammetric sensor for butylated hydroxyanisole based on AuNPs–PVP–graphene nanocomposites

“…In the one-pot method, both PVP-functionalized GO and 4 AuCl − were reduced simultaneously by ascorbic acid at 90 °C to obtain the Au-PVP-graphene hybrids. Fig.…”

“…Thus, it is necessary for people to determine reliably the amounts of BHA in food or other products to ensure human health. Many methods including spectrofluorimetry [3], gas chromatography [4], high-performance liquid chromatography [5], micellar electrokinetic capillary chromatography [6] and electrochemical methods [7][8][9][10][11][12][13] have been developed for determination of BHA. In contrast, electrochemical assays have been proposed as efficient alternative for the determination of BHA on account of their high sensitivity, low cost, and simplicity.…”

A molecularly-imprinted electrochemical sensor based on a graphene–Prussian blue composite-modified glassy carbon electrode for the detection of butylated hydroxyanisole in foodstuffs

“…6 Thus, it is necessary for people to determine reliably the amounts of BHA in food or other products to ensure our health. Nowadays, determination methods for BHA have been reported to include gas chromatographic, 7 high-performance liquid chromatography (HPLC), ow injection analysis, and so on. 8,9 However, these reported methods for the determination of BHA usually require complex pretreatment steps, long response times and expensive instrumental equipment.…”