A Rapid HPLC Method for the Concurrent Determination of Several Antihypertensive Drugs from Binary and Ternary Formulations

Attimarad, Mahesh; Venugopala, Katharigatta N.; Sreeharsha, Nagaraja; Chohan, Muhammad Shahzad; Shafi, Sheeba; Nair, Anroop B.; Shinu, Pottathil

doi:10.3390/separations8060086

Cited by 9 publications

(9 citation statements)

References 37 publications

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“…For quality control, a suitable analytical approach for assaying these anti-diabetic medicines is necessary. All laboratories are interested in developing easy, rapid, and reliable analytical processes for analyzing pharmaceuticals in various formulations, which can be accomplished by estimating a larger number of medications using a single approach employing the same stationary and mobile phases [21]. For the measurement of remogliflozin alone and in combination with other antidiabetic medications, only a few analytical procedures have been established.…”

Section: Introductionmentioning

confidence: 99%

Experimental Design Approach for Quantitative Expressions of Simultaneous Quantification of Two Binary Formulations Containing Remogliflozin and Gliptins by RP-HPLC

et al. 2022

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The aim of this study was to develop a fast RP-HPLC method for simultaneous measurement of two antidiabetic formulations (vildagliptin + remogliflozin and teneligliptin + remogliflozin) under identical experimental conditions. Using the Box–Behnken approach and response surface design, the interaction and quadratic influence of three variable parameters, acetonitrile %, pH of the mobile phase, and flow rate, on resolution between the peaks were optimized. To forecast the resolution of peaks (2.7 and 6.5) for the three anti-diabetic medications, the design space with desirability function was used to find the optimal chromatographic conditions. Isocratic elution with 58:42 acetonitrile and phosphate buffer (20 mM KH2PO4, pH adjusted to 4.9 with orthophosphoric acid) over a Zorabx C18 HPLC column with a flow rate of 1.2 mL min−1 separated all three analytes in 2.5 min. In addition, the optimized HPLC process was validated using ICH recommendations. The devised HPLC method’s precision and accuracy were proven by the low percent relative standard deviation (0.60–1.65%), good percentage recovery (98.18–101.50%), and low percentage relative errors (0.20–1.82%). The method’s robustness was also proven by slightly varying the five separate parameters. Finally, the accuracy of the proposed HPLC approach was confirmed using a standard addition method for simultaneous determination of vildagliptin + remogliflozin and teneligliptin + remogliflozin from formulations. Furthermore, the findings demonstrated that experimental design can be successfully used to optimize chromatographic conditions with fewer runs. The devised HPLC method for simultaneous quantification of two binary combinations utilizing the same chromatographic conditions is fast, accurate, precise, and easy, and it might be utilized in laboratories for routine quality control investigations on both formulations.

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Section: Introductionmentioning

confidence: 99%

Experimental Design Approach for Quantitative Expressions of Simultaneous Quantification of Two Binary Formulations Containing Remogliflozin and Gliptins by RP-HPLC

et al. 2022

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“…Pareto charts evident that the factors of organic phase composition, flow rate, and buffer pH have a profound influence on response factors, peak area, retention time, and tailing factor (Figure 3A–C). The ‘t’ value of the factors was between t value limit and Bonferroni limit, which indicate the significant influence of the factors [33]. From the screening study, it was found flow rate, the organic phase composition, and buffer pH had a significant ( p < 0.05) influence on CAAs, hence they were chosen as CMP for the optimization study.…”

Section: Resultsmentioning

confidence: 99%

Estimation of rutin‐loaded chitosan sodium alginate nanoparticles in rat plasma using a chemometrics‐assisted bioanalytical high‐performance liquid chromatography method

Palei

Vijayaraj²,

Shanmugam

et al. 2023

Separation Science Plus

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Rutin, a flavonoid glycoside is mostly made up of phenolic compounds and is involved in a variety of biological processes. Rutin-loaded chitosan-alginate nanoparticles were prepared for enhancing the site specificity and bioavailability. The main focus of the current research work was to develop and validate a simple, accurate, and robust bioanalytical reverse-phase high-performance liquid chromatography method using the Design of Experiments principles for the estimation of rutin-loaded chitosan-alginate nanoparticles in plasma. A mixture of methanol and phosphate buffer in the ratio of 45:55 v/v was used as the mobile phase. The pH of the mobile phase is adjusted to 3.2 with 80% orthophosphoric acid; the flow rate was optimized to 1.0 ml/min and the ultraviolet absorption wavelength at 254 nm. Critical analytical attributes were screened with Taguchi orthogonal array model design. To develop and analyze a "Design Space," Box-Behnken three-level, three-factorial design in the design of experiment was employed. Analysis of variance, contour plots, and three-dimensional surface response plots were used to statistically assess this design, and the model was shown to be statistically significant. The devised approach was validated according to the recommendations of the International Council for Harmonization (Q2 R1). The linearity of the developed method showed excellent in the range of 100 to 900 ng/ml with good regression (R 2 > 0.998), the limit of quantitation (30 ng/ml), and the limit of detection (10 ng/ml). The validation findings of the parameters were examined and found to be within acceptable limits. The study findings stated that the Quality by Design-driven bioanalytical approach is applicable for the in vivo and in vitro quantification of rutin derived from bulk and rutin-loaded chitosan-alginate nanoparticles formation.

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“…The percentage assay obtained was in the range of 98% to 102% for all the analytes. The method offered good linearity in the concentration of 2-30 μg/mL for Hydrochlorothiazide, 1-15 μg/mL for Amlodipine, 5-160 μg/mL for Olmesartan and Telmisartan and 10-300 μg/mL for Irbesartan [44] .…”

Section: Issn: 2320-4850 [90] Coden (Usa): Ajprhsmentioning

confidence: 93%

A Review on Simultaneous Determination of Anti-Hypertensive Drug Combinations Containing Amlodipine Besylate using Chromatographic Analysis

Chandran¹

2022

Asian J Pharm Res Dev

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The present review aims to investigate some of the recently reported analytical methods available for the assay ofantihypertensive drugs in various combinations of pharmaceutical forms. Several simultaneous RP- HPLC methods have beenreported for the determination of antihypertensive drugs. The review familiarizes various reported methods for the simultaneousdetermination of Amlodipine besylate with other antihypertensive drug formulations which are used in combination treatmentprotocols.

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A Rapid HPLC Method for the Concurrent Determination of Several Antihypertensive Drugs from Binary and Ternary Formulations

Cited by 9 publications

References 37 publications

Experimental Design Approach for Quantitative Expressions of Simultaneous Quantification of Two Binary Formulations Containing Remogliflozin and Gliptins by RP-HPLC

Experimental Design Approach for Quantitative Expressions of Simultaneous Quantification of Two Binary Formulations Containing Remogliflozin and Gliptins by RP-HPLC

Estimation of rutin‐loaded chitosan sodium alginate nanoparticles in rat plasma using a chemometrics‐assisted bioanalytical high‐performance liquid chromatography method

A Review on Simultaneous Determination of Anti-Hypertensive Drug Combinations Containing Amlodipine Besylate using Chromatographic Analysis

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