A rapid immunochromatographic test strip for detecting rabies virus antibody

Abstract

An immunochromatographic test strip (ICTS) for detecting antibodies to rabies virus was developed, using colloidal gold particles labeled with rabies virus glycoprotein as the tracer. The assay was evaluated using sera from dogs immunized with various commercial rabies vaccines, or from dogs in the clinics and sera from dogs immunized with vaccines against pathogens other than rabies virus, and negative sera from a wide variety of animal sources, including dogs, mice, and cats which had never been vaccinated. The ICTS was found to be highly specific for antibodies against rabies virus, with a detection limit of 0.5IU/ml as measured by the fluorescent antibody virus neutralization (FAVN) test. Compared with the FAVN test, the specificity and sensitivity of ICTS were 98.2% and 90.4%, respectively. There was an excellent agreement between results obtained by the ICTS and FAVN tests (kappa=0.888). Strips stored at 4°C in a plastic bag with a desiccant retained their specificity and sensitivity for at least 15 months, and strips stored at ambient temperature remained stable for 12 months. The immunochromatographic test strip may therefore be useful for clinical laboratories lacking specialized equipment and for diagnosis in the field for rapid detection of rabies virus-specific antibodies.

A Comparative Study of the RAPINA and the Virus‐Neutralizing Test (RFFIT) for the Estimation of Antirabies‐Neutralizing Antibody Levels in Dog Samples

“…Recently, several alternatives to the RFFIT and FAVN test have been developed to estimate VNA titres (Shiota et al., ) (Ma et al., ) (Wang et al., ) (Batista et al., ) (Madhusudana et al., ). As one of the alternative methods, we recently developed the RAPINA, which is an easy, rapid and inexpensive immunochromatography‐based competitive test using formalin‐fixed virus particles for the detection of RABV G protein‐specific antibodies (Shiota et al., ) (Nishizono et al., ) (Nishizono, ).…”

“…Similar to our RAPINA, Wang et al. developed an immunochromatographic test strip (ICTS) that was constructed by coating colloidal gold‐RABV G antigen onto the test line to directly detect antibodies against RABV in serum (Wang et al., ). In the ICTS, the appearance of two bands on the strip indicates the presence of the neutralizing antibody (≥0.5 IU/mL) in the sample.…”

Evaluation of Rapid Neutralizing Antibody Detection Test against Rabies Virus in Human Sera

“…Recently, a novel method based on the principles of chromatography has been developed to determine NAb levels against rabies in a very short time [ 6 , 7 , 11 ]. Several prototype kits have been developed and evaluated, but, to date, most of these have been evaluated using dog sera [ 6 , 11 ]. The first version of RAPINA was developed and evaluated by Shiota et al in 2009.…”

Development of a colloidal gold immunochromatographic strip for the rapid detection of soft-shelled turtle systemic septicemia spherical virus

“…In contrast, the colloidal gold immunochromatographic strip assay has a number of advantages for diagnosis, including simplification, visual evaluation, on-site screening and fast results obtained within 5-10 min without any instrumentation (Cui et al, 2008;Yang et al, 2010;Wang et al, 2010;Kusano et al, 2014;Meng et al, 2014). The use of immunochromatographic strips (ICS) has becoming widespread for the rapid and on-site detection of toxins, heavy metals, proteins, pesticides, hormones, drugs and pathogens (Wang et al, 2005Huo et al, 2006;Li et al, 2009;Omidfar et al, 2011;Yang et al, 2011;Liu et al, 2012).…”