A rapid method for isolation of total DNA from pathogenic filamentous plant fungi

González-Mendoza, Daniel; Argumedo-Delira, Rosalba; Morales, Adriana; Pulido-Herrera, A; Cervantes-Díaz, Lourdes; Grimaldo-Juarez, Onecimo; Alarcón, Alejandro

doi:10.4238/vol9-1gmr680

Cited by 52 publications

(22 citation statements)

References 7 publications

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“…Many published protocols are available to ensure an efficient and reproducible method for DNA extraction from plants (revised by Demeke and Jenkins, 2010;Biswas and Biswas, 2011); from fungi (Chi et al, 2009;Feng et al, 2010;González-Mendoza et al, 2010;Niu et al, 2008;Zelaya-Molina et al, 2011;Zhang, Y. J. et al, 2010); and from soil (revised by Hirsch et al, 2010).…”

Section: Dna Extraction Methodsmentioning

confidence: 99%

Molecular Tools for Detection of Plant Pathogenic Fungi and Fungicide Resistance

Capote¹,

Pastrana²,

Aguado³

et al. 2012

Plant Pathology

107

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Section: Dna Extraction Methodsmentioning

confidence: 99%

Molecular Tools for Detection of Plant Pathogenic Fungi and Fungicide Resistance

Capote¹,

Pastrana²,

Aguado³

et al. 2012

Plant Pathology

107

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“…DNA from freeze dried mycelia was extracted using a modified method described by Gonzalez-Mendoza et al [21]. The DNA was quantified using the NanoDrop ® 2000 (NanoDrop Technologies, Wilmington, DE).…”

Section: Dna Extractionmentioning

confidence: 99%

Assessing the Genetic Diversity of <i>Alternaria Bataticola</i> in South Africa using Molecular Markers

Chalwe

Adebola

Pillay

2017

AJAS

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Sweetpotato (Ipomoea batatas) an economically important food crop is affected by viral and fungal diseases. The most important fungal disease is Alternaria blight caused by Alternaria bataticola. Alternaria blight can be controlled using fungicides and cultural practices in the short-term. A sustainable control measure is the development of resistant sweetpotato cultivars. A prerequisite to this approach is the knowledge of the genetic diversity of this fungal pathogen. This study assessed the genetic diversity of A. bataticola in South Africa using RAPD analysis, DNA sequencing and secondary structures of the ITS2 region. Samples were collected from 25 different localities and the pathogen was identified in the laboratory. Both RAPD and ITS2 sequence data showed that there is high levels of genetic diversity among the isolates of A. bataticola. Although the dendrograms generated from the RAPD and ITS2 sequences clustered some isolates according to their place of origin, the majority of isolates did not group according to their geographic origins. The predicted ITS2 secondary structure models were variable ranging from simple to complex. The unique secondary structures of each isolate can be used to identify and distinguish each of the isolates used in this study.Â Â

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“…The mycelial mats were pelleted by centrifugation at 3000 rpm for 5 min at 4°C and the supernatant was carefully removed. Extraction of genomic DNA was carried out following the 3% SDS method (Gonzalez-Mendoza et al 2010). The concentration of obtained genomic DNA was measured using a NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, USA).…”

Section: Small Segments (5 MMmentioning

confidence: 99%

Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia.

Abed-Ashtiani¹,

Kadir²,

Nasehi³

et al. 2016

Czech J. Genet. Plant Breed.

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Abed-Ashtiani F., Kadir J., Nasehi A., Hashemian-Rahaghi S.-R., Vadamalai G., Rambe S.-K. (2016): Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia. Czech J. Genet. Plant Breed., 52: 145-156.The genus Pyricularia (anamorph)/Magnaporthe (teleomorph) includes important destructive pathogens causing blast disease on various species from the family Poaceae. Thirty-five Magnaporthe isolates were collected from diseased rice plants (variety MR219) in different rice-growing regions of Malaysia, including the five states of Selangor, Penang, Kedah, Kelantan, and Perak between 2010 and 2014. DNA sequence analyses of the internal transcribed spacer (ITS), actin, β-tubulin and calmodulin gene regions, random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses were conducted to analyse 35 Magnaporthe isolates. Phylogenetic analysis of the combined dataset confirmed the identification of all isolates as M. oryzae with a high distance from other Magnaporthe (Pyricularia) species. RAPD and ISSR analyses indicated the existence of a relatively low similarity index value among M. oryzae isolates through identification of four main clades. The clustering of RAPD and ISSR analyses demonstrated that there was a correlation between the isolates and their geographical origins.

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A rapid method for isolation of total DNA from pathogenic filamentous plant fungi

Cited by 52 publications

References 7 publications

Molecular Tools for Detection of Plant Pathogenic Fungi and Fungicide Resistance

Molecular Tools for Detection of Plant Pathogenic Fungi and Fungicide Resistance

Assessing the Genetic Diversity of <i>Alternaria Bataticola</i> in South Africa using Molecular Markers

Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia.

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