A rapid method for ploidy determination in fungal cells

Talbot, Nicholas J.; Rawlins, David J.; Coddington, Alan

doi:10.1007/bf00405853

Cited by 14 publications

(3 citation statements)

References 5 publications

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“…Similar results have been seen when comparing known haploid and diploid populations of Sehizosaecharomyees pombe (Talbot et al 1988). Similar results have been seen when comparing known haploid and diploid populations of Sehizosaecharomyees pombe (Talbot et al 1988).…”

Section: Resultssupporting

confidence: 81%

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Increase in ploidy in yeasts as a response to stressing media

Talbot

Wayman

1989

Appl Microbiol Biotechnol

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A recently developed technique for determining the ploidy of fungal cells has been used to examine the relative ploidy levels of culture collection strains of two xylose-fermenting yeasts Candida shehatae and Pichia stipitis and their respective R strains. The R strains have been shown to have increased ploidy by at least a factor of two during their recycling in stressing media, namely wood hydrolysates. Their improved efficiency in xylose fermentation may be attributed at least in part to their increased ploidy.

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Section: Resultssupporting

confidence: 81%

“…Ploidy determination followed the methods described by Talbot et al (1988). Ploidy determination followed the methods described by Talbot et al (1988).…”

Section: Methodsmentioning

confidence: 99%

Increase in ploidy in yeasts as a response to stressing media

Talbot

Wayman

1989

Appl Microbiol Biotechnol

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“…Our analysis of random spore progeny rather than complete tetrads has allowed us to overcome the otherwise considerable difficulty that poor spore viability poses, while use of a positive selection to obtain hybrids (Spencer and Spencer, 1980) has eliminated the need to isolate mating-competent spores. The method is not foolproof, but is more sensitive to the consequences of aneuploidy than methods based on determinations of DNA content (Leusch et al, 1985;Talbot et al, 1988) or cell volume. The latter procedures are more applicable to closely related euploid strains in which the number of chromosomes differs by factors of at least 2, rather than some fractional value which may be of the same order of magnitude as the variance…”

Section: Introductionmentioning

confidence: 99%

The chromosomal constitution of wine strains of Saccharomyces cerevisiae

Bakalinsky

Snow

1990

Yeast

133

102

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A general procedure is described for determining the chromosomal constitution of industrial strains of Saccharomyces cerevisiae based on analysis of segregation frequencies for input markers among random spore progeny of industrial-laboratory strain hybrids. The multiply auxotrophic haploid testers used carried a dominant erythromycin-resistance marker, allowing hybrids to be selected in mass matings with spores produced by the wild-type industrial strains. Analysis of a number of independent crosses between the haploid testers and an unselected population of spores of each wine strain distinguished between disomic, trisomic and tetrasomic chromosomal complements in the parents. Possible explanations for a significant class of aberrant segregation frequencies are discussed. Results of the analysis indicate that UCD Enology 522 (Montrachet) is diploid and possibly trisomic for chromosome VII; 522X is diploid; UCD Enology 505 (California Champagne) is disomic for chromosome XVI, trisomic for chromosomes I, II, III, VI, VIII, IX, X, XII, XV, tetrasomic for chromosomes IV, XI, XIII, XIV and either trisomic or tetrasomic for chromosomes V and VII; and that UCD Enology 595 (Pasteur Champagne) is disomic for chromosomes I, II, III, IX, XVI, trisomic for chromosomes IV, VI, X, XII, XIV, XV, tetrasomic for chromosomes V, VIII, XI, XIII and either disomic or tetrasomic for chromosome VII.

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Determination of the Relative Ploidy in DifferentSaccharomyces cerevisiae Strains used for Fermentation and ‘Flor’ Film Ageing of Dry Sherry-type Wines

Guijo

Mauricio

Salmon

et al. 1997

Yeast

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The full chromosomal karyotype of six enological Saccharomyces cerevisiae strains used for fermentation and biological ageing of sherry‐type wines was studied. A genetic method based on the analysis of segregation frequencies of auxotrophic markers, among random spore progeny of hybrids, constructed between laboratory and industrial wine strains (Bakalinsky and Snow, 1990) was used. This method was combined with the analysis of strains by pulsed‐field gel electrophoresis. The results obtained clearly indicate the presence of two, three or four copies of a chromosome in the industrial strains examined, and thus confirm that aneuploidy/polyploidy is not uncommon in these strains. In all strains examined, chromosome XIII polysomy is observed. This chromosome contains the ADH2 and ADH3 loci, that code for the ADHII and ADHIII isoenzymes of alcohol dehydrogenase, which are involved in ethanol oxidative utilization during biological ageing of wines. Tetrad analysis for the ‘flor formation’ character suggests two possibilities: this character is either regulated by at least a digenic system, or by only one gene present on a chromosome which is, at least, disomic.© 1997 John Wiley & Sons, Ltd.

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A rapid method for ploidy determination in fungal cells

Cited by 14 publications

References 5 publications

Increase in ploidy in yeasts as a response to stressing media

Increase in ploidy in yeasts as a response to stressing media

The chromosomal constitution of wine strains of Saccharomyces cerevisiae

Determination of the Relative Ploidy in DifferentSaccharomyces cerevisiae Strains used for Fermentation and ‘Flor’ Film Ageing of Dry Sherry-type Wines

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