A Rapid Method for the Pre-Enrichment and Detection ofSalmonella Typhimurium by Immunomagnetic Separation and Subsequent Fluorescence Microscopical Techniques

Steingroewer, Juliane; Knaus, Helene; Bley, Thomas; Boschke, Elke

doi:10.1002/elsc.200420072

Cited by 15 publications

(9 citation statements)

References 6 publications

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“…Here separation efficiencies of 98% in pure phosphate buffer and 92% with bovine serum albumin as blocking solution were reached. This result is comparable with magnetic cell separation using antibodies as ligands [4,7].…”

Section: Resultssupporting

confidence: 89%

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Biomagnetic separation of Salmonella Typhimurium with high affine and specific ligand peptides isolated by phage display technique

Steingroewer

Bley

Bergemann

et al. 2007

Journal of Magnetism and Magnetic Materials

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Section: Resultssupporting

confidence: 89%

“…Biomagnetic separation (BMS) is an alternative method for rapid pre-enrichment of bacteria [2][3][4]. A special form of BMS is the immunomagnetic separation (IMS).…”

Section: Introductionmentioning

confidence: 99%

Biomagnetic separation of Salmonella Typhimurium with high affine and specific ligand peptides isolated by phage display technique

Steingroewer

Bley

Bergemann

et al. 2007

Journal of Magnetism and Magnetic Materials

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“…We see the main reason for the still lower separation efficiency in the rather high values of the relative velocity between the particles and cells, which probably reduce the binding efficiency p in Eq. (1). The investigation of the binding process depending on the relative velocity and fluid behavior is the subject of further studies.…”

Section: Discussionmentioning

confidence: 99%

A new method for mixing of suspended superparamagnetic beads using variable electromagnetic fields

Stange

Lenk

Eckert

et al. 2015

Engineering in Life Sciences

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The detection, identification, and analysis of bacterial pathogens are highly important for identifying potential health risks. Often, the first step of the process is an enrichment of the pathogens to such densities that exceed the detection limits of analytical instruments. In this context, biomagnetic separation is a promising approach to achieve this enrichment due to its selectivity and efficiency. Here, we present the electromagnetic sample mixer (ESM), an innovative device that may optimize this process. The aim of the ESM is to improve the binding between the superparamagnetic particles (beads) and the intended biological targets through the movement of the beads using variable magnetic fields to reduce these costly beads and save process time. The magnetic fields are generated using three electromagnets equipped with magnetic cores with special pole shoe geometry. This geometry was improved by numerical simulation of the magnetic field using FEMLAB 3. Also, proof-of-principle studies with Escherichia coli were performed by comparing the separation efficiencies received using the ESM and the conventional Dynabeads MX4 Mixer (Life Technologies AG, Carlsbad, CA, USA). The results show a similar performance of these two devices.

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“…In food microbiology, BMS has been extensively studied for the specific isolation of pathogenic microorganisms which require methods with a very low detection limit, such as Salmonella spp., Listeria monocytogenes, or Escherichia coli O157:H7, from a huge variety of samples such as culture media, water, and foodstuff samples [16][17][18][19][20][21]. Specific polyclonal and monoclonal antibodies are the most frequently used ligands in this field.…”

Section: Introductionmentioning

confidence: 99%

Specific peptides as alternative to antibody ligands for biomagnetic separation of Clostridium tyrobutyricum spores

et al. 2011

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Nowadays, the reference method for the detection of Clostridium tyrobutyricum in milk is the most-probable-number method, a very time-consuming and non-specific method. In this work, the suitability of the use of superparamagnetic beads coated with specific antibodies and peptides for bioseparation and concentration of spores of C. tyrobutyricum has been assessed. Peptide or antibody functionalized nanoparticles were able to specifically bind C. tyrobutyricum spores and concentrate them up to detectable levels. Moreover, several factors, such as particle size (200 nm and 1 μm), particle derivatization (aminated and carboxylated beads), coating method, and type of ligand have been studied in order to establish the most appropriate conditions for spore separation. Results show that concentration of spore is favored by a smaller bead size due to the wider surface of interaction in relation to particle volume. Antibody orientation, related to the binding method, is also critical in spore recovery. However, specific peptides seem to be a better ligand than antibodies, not only due to the higher recovery ratio of spores obtained but also due to the prolonged stability over time, allowing an optimal recovery of spores up to 3 weeks after bead coating. These results demonstrate that specific peptides bound to magnetic nanoparticles can be used instead of traditional antibodies to specifically bind C. tyrobutyricum spores being a potential basis for a rapid method to detect this bacterial target.

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A Rapid Method for the Pre-Enrichment and Detection ofSalmonella Typhimurium by Immunomagnetic Separation and Subsequent Fluorescence Microscopical Techniques

Cited by 15 publications

References 6 publications

Biomagnetic separation of Salmonella Typhimurium with high affine and specific ligand peptides isolated by phage display technique

Biomagnetic separation of Salmonella Typhimurium with high affine and specific ligand peptides isolated by phage display technique

A new method for mixing of suspended superparamagnetic beads using variable electromagnetic fields

Specific peptides as alternative to antibody ligands for biomagnetic separation of Clostridium tyrobutyricum spores

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