A Rapid Method for the Measurement of Oestriol in Pregnancy Plasma by Competitive Protein Binding Analysis

Summary Total plasma oestriol in pregnancy was estimated by a competitive protein binding method. The advantages of the measurement of plasma oestriol levels over the 24‐ or 48‐hour urinary oestriol assays are discussed. The pattern of plasma oestriol levels in the third trimester of pregnancy was investigated in over 400 healthy women and in general a uniform increase was found from the 28th week until term. Diurnal variation was found to be present, but this did not preclude the use of the method for assessing the feto‐placental unit. Exercise and meals had a minimal effect on the oestriol levels. Following delivery the level decreased sharply to reach, within four hours, one‐half that found just prior to delivery.

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“…The collection of specimens and the assay procedure has been described in detail by Corker and Naftolin (1971).…”

Section: Methodsmentioning

confidence: 54%

Plasma Oestriol in Pregnancy

Macourt

Corker²,

Naftolin³

1971

BJOG

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“…Results in patients having spontaneous delivery and elective Caesarean section are shown in Tables IV and V. (Corker and Naftolin, 1971). Smith (1966), using yet a different method of assay, found similar levels of oestriol after the 35th week of gestation.…”

Section: Plasma Levels At the Time Of Deliverymentioning

confidence: 99%

Maternal and Fetal Venous Plasma Steroids in Relation to Parturition

Shearman

Jools

Smith

1972

BJOG

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This paper presents the results obtained from the study of oestriol and pregnanediol in maternal peripheral venous plasma throughout normal pregnancy. Changes of oestriol, pregnanediol and 11-hydroxysteroid levels throughout the day in late pregnancy were studied. The levels of these steroids found in maternal peripheral venous blood and umbilical venous blood in patients delivered normally and by elective Caesarean section have been compared. The range of values for plasma pregnanediol was so large that further study had little to recommend it. In a relative sense there was good agreement between plasma oestriol and urinary 24-hour oestriol excretion. There was no difference in fetal plasma levels of oestriol, pregnanediol or 11-hydroxysteroids when babies delivered vaginally were compared with those delivered by elective Caesarean section. The difference in maternal 11-hydroxysteroids in these two situations approached significance.

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“…Since 1969 several different methods for estimation of plasma oestrogens employing competitive protein binding techniques have been published (9,10,15,32,33,34,46,56,60,64,65).…”

Section: Introductionmentioning

confidence: 99%

Radioimmunological Methods for the Estimation of Oestrone, Oestradiol 17β and Oestriol in Pregnancy Plasma

Lindberg

Martinsson

et al. 1974

Acta Obstet Gynecol Scand

126

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BY SUMMARY Antibodies to oestradiol-17p and oestriol have been produced by immunization of sheep with oestradiol-6-oxime and oestriol-6-oxime attached to bovine serum albumin (BSA). The antibodies were used in radioimmunoassays.In the oestradiol-17p assay the free and bound steroids were separated by means of dextrancoated charcoal. This technique caused a heavy stripping when tried in the oestriol assay. A new assay for oestriol in plasma was thus devised. In this assay, oestriol and antibody were incubated at 4OoC for 30 minutes. Free and bound steroids were separated with saturated ammonium sulphate. The specificity of the antibody raised after immunization with oestradiol-17p-6-oxirne BSA was compared with apreviously used antibody obtained after conjugation in the 17th position of oestradiol-17p to BSA. The former antibody had a higher specificity and crossreacted only 11 per cent with oestrone compared to 50 per cent for the latter. The oestriol antibody was highly specific, crossreacting only 13 per cent with oestetrol and 5.8 per cent with 16-epioestriol.The radioimmunoassay procedures when applied to pregnancy plasma were evaluated with regard to specificity, sensitivity, accuracy, precision and blank factors. The results were compared to those obtained after Sephadex LH-20 chromatography and establish the advantages of highly specific antibodies over the time consuming Sephadex LH-20 chromatography.

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A Rapid Method for the Measurement of Oestriol in Pregnancy Plasma by Competitive Protein Binding Analysis

Cited by 29 publications

References 12 publications

Plasma Oestriol in Pregnancy

Plasma Oestriol in Pregnancy

Maternal and Fetal Venous Plasma Steroids in Relation to Parturition

Radioimmunological Methods for the Estimation of Oestrone, Oestradiol 17β and Oestriol in Pregnancy Plasma

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