A rapid method to screen wild Solanum for resistance to early blight

Abstract

Early blight of potato and tomato is caused by Alternaria fungi and negatively impacts crop yields. Environmental factors and plant maturity influence disease development, which is usually kept under control by fungicide applications. Wild tuber-bearing Solanum section Petota species are a promising source of resistance to early blight that could be used to control the disease, for example by crossbreeding or modern breeding approaches. An efficient screening method is a first prerequisite for the identification of resistant genotypes in wild Solanum germplasm. Here, we describe a protocol that can be used to rapidly screen for resistance to early blight in wild Solanum collections. This protocol provides a good starting point for the identification of resistant genotypes and is a step towards breeding for resistance to early blight using wild Solanum species.

Review: Holistic pest management against early blight disease towards sustainable agriculture

“…Wild genotypes of potato and tomato in the Solanum genus, originally from South America, are a beneficial resource of germplasm resistance to EB disease 40–42 . The genotypes that belong to S. habrochaites and S. arcanum were found to be moderately or highly resistant to EB 5,43 .…”

“…Wild genotypes of potato and tomato in the Solanum genus, originally from South America, are a beneficial resource of germplasm resistance to EB disease. 40 , 41 , 42 The genotypes that belong to S. habrochaites and S. arcanum were found to be moderately or highly resistant to EB. 5 , 43 The resistant wild‐type cultivars strongly activate several pathways of plant response, including glycol‐alkaloid, flavonoid and lignin biosynthesis by up‐regulating gene expressions.…”

Resistance of Wild Solanum Species and Hybrids to Early and Late Blight

“…Наиболее надежным методом является лабораторная оценка устойчивости, обеспечивающая равномерное нанесение инокулюма на листья растений. Недавно проведенными исследованиями показано, что степень поражения, вызванная действием грибов Alternaria на растения диких видов Solanum, варьирует в зависимости от вида и штамма патогена (Wolters et al, 2019). Авторы наблюдали большие различия по степени поражения S. microdontum subsp.…”

“…В тестах заражения листьев образцов картофеля с использованием изолятов P. infestans, различающихся по признаку наличия гена вирулентности v.2, степень устойчивости одних и тех же сеянцев существенно различалась (Zoteyeva, 2000 Генетические источники частичной устойчивости к альтернариозу, которые можно использовать в селекционных программах, были выявлены среди диких видов картофеля Solanum immite Dunal, Solanum polyadenium Greenm. (Wolters et al, 2019) и Solanum raphanifolium Cardenas & Hawkes (Weber, Jansky, 2012). В проведенном нами многолетнем исследовании виды S. immite и S. polyadenium также проявляли высокую устойчивость к фитофторозу в полевых условиях (Зотеева, 2019).…”

Qualitative and Quantitative Resistance against Early Blight Introgressed in Potato

“…They have been extensively studied to identify resistance genes against potato pathogens, especially Phytophthora infestans [ 14 , 15 ]. Wild potato relatives are a promising source of resistance against A. solani too [ 16 , 17 , 18 , 19 , 20 ], but the genes that underly resistance remain to be identified.…”