Qualitative and Quantitative Resistance against Early Blight Introgressed in Potato

Wolters, Pieter J.; Wouters, Doret; Kromhout, Emil J.; Huigen, D. J.; Visser, R.G.F.; Vleeshouwers, Vivianne G. A. A.

doi:10.3390/biology10090892

Cited by 18 publications

(18 citation statements)

References 55 publications

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“…Based on previous studies on A. solani , we selected a relatively stable housekeeping gene " Actin " as an internal reference gene [ 24 , 25 ]. Specific primers for Actin and AsCEP112 were designed using the National Center for Biotechnology Information (NCBI) database ( https://www.ncbi.nlm.nih.gov/ ) (Supplementary Table S 2 ).…”

Section: Methodsmentioning

confidence: 99%

Identification of effector CEP112 that promotes the infection of necrotrophic Alternaria solani

et al. 2022

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Background Alternaria solani is a typical necrotrophic pathogen that can cause severe early blight on Solanaceae crops and cause ring disease on plant leaves. Phytopathogens produce secretory effectors that regulate the host immune response and promote pathogenic infection. Effector proteins, as specialized secretions of host-infecting pathogens, play important roles in disrupting host defense systems. At present, the role of the effector secreted by A. solani during infection remains unclear. We report the identification and characterization of AsCEP112, an effector required for A. solani virulence. Result The AsCEP112 gene was screened from the transcriptome and genome of A. solani on the basis of typical effector signatures. Fluorescence quantification and transient expression analysis showed that the expression level of AsCEP112 continued to increase during infection. The protein localized to the cell membrane of Nicotiana benthamiana and regulated senescence-related genes, resulting in the chlorosis of N. benthamiana and tomato leaves. Moreover, comparative analysis of AsCEP112 mutant obtained by homologous recombination with wild-type and revertant strains indicated that AsCEP112 gene played an active role in regulating melanin formation and penetration in the pathogen. Deletion of AsCEP112 also reduced the pathogenicity of HWC-168. Conclusion Our findings demonstrate that AsCEP112 was an important effector protein that targeted host cell membranes. AsCEP112 regulateed host senescence-related genes to control host leaf senescence and chlorosis, and contribute to pathogen virulence.

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Section: Methodsmentioning

confidence: 99%

Identification of effector CEP112 that promotes the infection of necrotrophic Alternaria solani

et al. 2022

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“…Early blight is an important disease of tomato and potato that is caused by the necrotrophic fungal pathogen Alternaria solani (52-54). In a previous study, we found a wild potato species, Solanum commersonii , with strong resistance to A. solani (55). We showed that resistance is likely caused by a single dominant locus and that it can be introgressed in cultivated potato (55).…”

Section: Introductionmentioning

confidence: 97%

“…Resistance to necrotrophs is usually considered to be a complex, polygenic trait, or recessively inherited according to the inverse gene-for-gene model (56)(57)(58)(59)(60)(61). It therefore surprised us to find a qualitative dominant resistance against early blight in S. commersonii (55).…”

Section: Introductionmentioning

confidence: 99%

Tetraose steroidal glycoalkaloids from potato provide resistance againstAlternaria solaniand Colorado potato beetle

Wolters

Wouters

Tikunov

et al. 2022

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Plants with innate disease and pest resistance can contribute to more sustainable agriculture. Resistance breeding typically relies on the use of immune receptors or impaired susceptibility genes, but these come with challenges in terms of strength, durability or pleotropic effects. Natural defence compounds from plants are not considered in breeding, because biosynthesis of these compounds is a complex trait and proof of their role in resistance is often indirect. Here, we identified a wild relative of potato, Solanum commersonii, that provides us with unique insight in the role of glycoalkaloids in plant immunity. We cloned two atypical resistance genes that can provide complete resistance to Alternaria solani and Colorado potato beetle through the production of tetraose steroidal glycoalkaloids. Moreover, we show that these compounds are active against a wide variety of fungi. This research highlights the potential of using natural defence compounds produced by plants to protect them against pests and diseases.

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“…In these previous studies, resistance levels showed a continuous distribution, suggesting that there were no dominant resistance genes for early blight resistance in these populations. However, Wolters et al (2021) recently found early blight dominant genetic resistance from the cross between S. commerosum subp. malmeanum and diploid S. tuberosum.…”

Section: Introductionmentioning

confidence: 99%

Genetic Dissection of Early Blight Resistance in Tetraploid Potato

et al. 2022

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Early blight, caused by the fungus Alternaria solani, is one of the most economically important diseases of potatoes worldwide. We previously identified a tetraploid potato clone, B0692-4, which is resistant to early blight. To dissect the genetic basis of early blight resistance in this clone, a full-sib tetraploid potato population including 241 progenies was derived from a cross between B0692-4 and a susceptible cultivar, Harley Blackwell, in this study. The population was evaluated for foliage resistance against early blight in field trials in Pennsylvania in 2018 and 2019 and relative area under the disease progress curve (rAUDPC) was determined. The distribution of rAUDPC ranged from 0.016 to 0.679 in 2018, and from 0.017 to 0.554 in 2019. Broad sense heritability for resistance, as measured as rAUDPC, was estimated as 0.66–0.80. The population was also evaluated for foliar maturity in field trials in Maine in 2018 and 2020. A moderate negative correlation between rAUDPC and foliar maturity was detected in both years. A genetic linkage map covering a length of 1469.34 cM with 9124 SNP markers was used for mapping quantitative trait loci (QTL) for rAUDPC and foliar maturity. In 2018, three QTLs for early blight were detected; two of them on chromosome 5 overlapped with QTLs for maturity, and one of them on chromosome 7 was independent of maturity QTL. In 2019, six QTLs for early blight were detected; two QTLs on chromosome 5 overlapped with QTLs for maturity, and the other four QTLs did not overlap with QTLs for maturity. The identification of these QTLs provides new insight into the genetic basis of early blight resistance and may serve as sources for marker-assisted selection for early blight resistance breeding.

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Qualitative and Quantitative Resistance against Early Blight Introgressed in Potato

Cited by 18 publications

References 55 publications

Identification of effector CEP112 that promotes the infection of necrotrophic Alternaria solani

Identification of effector CEP112 that promotes the infection of necrotrophic Alternaria solani

Tetraose steroidal glycoalkaloids from potato provide resistance againstAlternaria solaniand Colorado potato beetle

Genetic Dissection of Early Blight Resistance in Tetraploid Potato

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