A rapid micro-determination of neutral sugars and aminosugars in glycopeptides by thin-layer chromatography

Móczár, E.; Moczar, M; Schillinger, Gerhard; Robert, L.

doi:10.1016/s0021-9673(01)86115-x

Cited by 83 publications

(16 citation statements)

References 6 publications

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“…HA, as approximately 100 /i,g of uronic acid, was digested with Streptomyces hyaluronidase (15 turbidity reducing units) in 50 fxA of 0.1 M acetate buffer, pH 5.0, at 37°C for 120 min. 40 HS, as 300 ^g of uronic acid, was digested with heparitinase (1 unit) in 70 pil of 0.1 M acetate buffer, pH 7.0, at 42°C for 18 h. 35 Identification of hexosamine moieties was carried out by thin-layer chromatbgraphy, using Eastman Kodak 511 sheets by the method of Moczar et al 42 The unsaturated disaccharides derived from the CS isomers by digestion with chondroitinases were applied as a band 1 cm in width to Whatman No. 1 filter paper.…”

Section: Enzymatic Assay Methods For Agag Componentsmentioning

confidence: 99%

Distribution of acidic glycosaminoglycans, lipids and water in normal human cerebral arteries at various ages.

Murata¹

1985

Stroke

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SUMMARY Functional alterations in arterial acidic glycosaminoglycans (AGAG) may be related to the pathogenesis of some forms of cerebrovascular disease. We measured the AGAG, lipid and water content of human cerebral artery of 275 normal males at various ages. These measures were separately carried out in the main trunk and distal branches. The AGAG components were analyzed by an enzymatic assay method employing specific enzymes which digest AGAG to assess topographic change and aging variations. The total AGAG content was higher in the main cerebral artery than in the distal branches. The main AGAG component of the normal main cerebral artery was heparan sulfates (HS), constituting half the total AGAG, followed by moderate amounts of dermatan sulfate (DS), chondroitin-6-sulfate (C-6S) and chondroitin-4-sulfate (C-4S). Hyaluronic acid (HA) was a minor component and it was more prominent in young arteries. Heparin could be occasionally detected. With advancing age, the relative amounts of HS, HA, chondroitin and C-4S both in the main trunk and distal branches decreased but those of DS and C-6S increased. The total lipid, cholesterol ester and triglyceride content was greater in the main trunk than in the distal branches; the total lipid content increased with age. A possible function of the cerebral arterial AGAG is discussed with respect to change in lipid and water content according to topographic sites and aging.

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Section: Enzymatic Assay Methods For Agag Componentsmentioning

confidence: 99%

Distribution of acidic glycosaminoglycans, lipids and water in normal human cerebral arteries at various ages.

Murata¹

1985

Stroke

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“…(1) Analysis of amino sugars: AMPS samples were hydrolyzed with 4 N HC1 for 4 hours at 110°C in sealed ampuls. HC1 was removed by evaporation under vacuum, and the nature of amino sugars was determined by thin layer ehromatography of the hydrolyzed sample, as described by Moczar et al 7 Glucosamine and galactosamine were used as standards. (2) Analysis of uronic acids: Uronic acid components of AMPS samples were identified by descending paper ehromatography of the hydrolyzed samples, as described by Radhakrisnamurthy and Berenson.…”

Section: Methodsmentioning

confidence: 99%

Comparative studies of the acid mucopolysaccharide composition of rheumatic and normal heart valves in man.

Baig¹,

Daicoff²,

Ayoub³

1978

Circulation Research

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SUMMARY The nature and relative proportions of acid mucopolysacharides (AMPS) in mitral valves of normal individuals and individuals with rheumatic valvular disease were compared. The total AMPS content of valves from rheumatic patients under 21 years of age is significantly higher than that from older rheumatic patients and higher than that from age-matched normal controls. The major AMPS components of both normal and rheumatic valvular tissue are hyaluronic acid, chondroitin sulfate A/C, and chondroitin sulfate B. Valves from normal individuals in all age groups studied contain approximately 65% hyaluronic acid, 25% chondroitin sulfate A/C, and 10% chondroitin sulfate B. Valves from rheumatic patients under 40 years of age contain the same proportions of these components as do normal valves. However, valves from rheumatic patients above 40 years of age show a marked alteration in AMPS composition; they contain 35% hyaluronic acid, 47% chondroitin sulfate A/C, and 18% chondroitin sulfate B. The differences in total AMPS content between valves from young rheumatic and nonrheumatic individuals may reflect changes that are secondary to the acute inflammatory process or an abnormality in AMPS synthesis following acute injury that is peculiar to the rheumatic host. The alterations in AMPS content and composition in valves of older rheumatic patients probably reflect changes in AMPS turnover associated with fibrosis that occurs in chronic disease of valvular tissue.

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“…NH40H (85:15) for hexosamines (Moczar et al, 1967). Monosaccharides were located with a naphthoresorcinolphosphoric acid stain and hexosamines with the Elson-Morgan reagent.…”

Section: Thin-layer Chromatographymentioning

confidence: 99%

The Swarming Of Proteus On Semisynthetic Media

Brogan¹,

Nettleton

Reid

1971

Journal of Medical Microbiology

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PLATES I-IVSWARMING by proteus on solid medium has been the subject of investigation since it was first described by Hauser (1885), as it is a phenomenon that does not occur to the same degree with other Gram-negative rods. Lominski and Lendrum (1947) suggested that swarming is a reaction by the organisms to toxic products of their own metabolism, a view supported by the work of Hughes (1957), who demonstrated that the forms in a swarm are morphologically indistinguishable from those produced by the damaging action of penicillin. A relation exists between the ability of proteus to swarm on a solid medium and the composition of the medium. It is common knowledge that the presence of bile salts inhibits swarming and a variety of toxic substances as diverse as chloral hydrate (Kramer and Koch, 1931) and sulphonamides (Holman, 1957) inhibit swarming when added to media. Naylor (1960) devised a medium that did not promote swarming of proteus, by omitting sodium chloride from the conventional formula of nutrient agar. Jones and Park (1967) found that growth but not swarming occurred on a solid medium based on the minimal medium of Fildes (1938), but these workers also claimed that swarming on this medium was promoted by the addition of various amino compounds.The present investigation is an attempt to define the components of solid media that are responsible for the promotion of swarming by proteus. MATERIALS AND METHODSOrganisms Proteus mirabilis NCTC5887 and 1 1 wild strains of Proteus mirabilis variously isolated from urinary infections, wounds, ulcers and rectal swabs were studied in the present investigation; the wild strains were identified as Proteus mirabilis by the criteria of Cowan and Steel (1965). The organisms were maintained in Robertson's cooked-meat broth at room temperature and were subcultured at monthly intervals. Before testing, the organisms were brought into the log phase of growth by inoculating them into 5 ml of nutrient broth and incubating for 5 hr at 37°C.

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A rapid micro-determination of neutral sugars and aminosugars in glycopeptides by thin-layer chromatography

Cited by 83 publications

References 6 publications

Distribution of acidic glycosaminoglycans, lipids and water in normal human cerebral arteries at various ages.

Distribution of acidic glycosaminoglycans, lipids and water in normal human cerebral arteries at various ages.

Comparative studies of the acid mucopolysaccharide composition of rheumatic and normal heart valves in man.

The Swarming Of Proteus On Semisynthetic Media

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