A rapid, one step molecular identification of Trichoderma citrinoviride and Trichoderma reesei

Saroj, Dina B; Dengeti, Shrinivas N.; Aher, Supriya; Gupta, Anil Kumar

doi:10.1007/s11274-015-1839-9

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…Quantitative real-time polymerase chain reaction (QPCR) was used to quantify the expression of target transcripts and DNA. Translation elongation factor alpha 1 ( TEF1 , KJ665454) was used to quantify T. citrinoviride using QPCR [21]. Primer 3 software from Biology Workbench (http://workbench.sdsc.edu/) was used to design primers (Table S1).…”

Section: Methodsmentioning

confidence: 99%

Endophytic Trichoderma citrinoviride isolated from mountain-cultivated ginseng (Panax ginseng) has great potential as a biocontrol agent against ginseng pathogens

Park

Mishra

Yoon

et al. 2019

Journal of Ginseng Research

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Background Ginseng ( Panax ginseng Meyer) is an invaluable medicinal plant containing various bioactive metabolites (e.g., ginsenosides). Owing to its long cultivation period, ginseng is vulnerable to various biotic constraints. Biological control using endophytes is an important alternative to chemical control. Methods In this study, endophytic Trichoderma citrinoviride PG87, isolated from mountain-cultivated ginseng, was evaluated for biocontrol activity against six major ginseng pathogens. T. citrinoviride exhibited antagonistic activity with mycoparasitism against all ginseng pathogens, with high endo-1,4-β-D-glucanase activity. Results T. citrinoviride inoculation significantly reduced the disease symptoms caused by Botrytis cinerea and Cylindrocarpon destructans and induced ginsenoside biosynthesis in ginseng plants. T. citrinoviride was formulated as dustable powder and granules. The formulated agents also exhibited significant biocontrol activity and induced ginsenosides production in the controlled environment and mountain area. Conclusion Our results revealed that T. citrinoviride has great potential as a biological control agent and elicitor of ginsenoside production.

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Section: Methodsmentioning

confidence: 99%

Endophytic Trichoderma citrinoviride isolated from mountain-cultivated ginseng (Panax ginseng) has great potential as a biocontrol agent against ginseng pathogens

Park

Mishra

Yoon

et al. 2019

Journal of Ginseng Research

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“…Most Trichoderma species were as biocontrol fungus widely using to control soil-borne diseases. In order to rapidly identify Trichoderma species, species-speci c primers pairs such as Th1F/Th1R for T. harzianum, Z04_2F/Z04_2R for T. atroviride, and Tc_RIF/ T_RIR for T. citrinoviride were developed in recently years (Prabhakaran et al 2015;Skoneczny et al 2015;Saroj et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…For the rapid identi cation of Trichoderma species, the ITS region, tef1 gene, and rpb2 gene are using to be the barcode locus for designing speci c primers. So far, several species-speci c primers have been developed for Trichoderma species identi cation (Friedl and Druzhinina 2012;Prabhakaran et al 2015;Saroj et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Development of Species/Genus specific Primers for Identification of Three Trichoderma Species and for Detection of Trichoderma Genus

Zhou

Wang²,

Yang

et al. 2021

Preprint

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Trichoderma is a widely used bio-control agent, and has excellent ability to antagonize plant pathogens and promoting plant growth. It has been successfully used to control various plant diseases. The premise of using Trichoderma is accurate identification of it. In this study, four sets of species/genus-specific primers were designed based on the translation elongation factor 1-alpha (tef1) gene and internal transcribed spacer (ITS) region, in order to identify Trichoderma harzianum, T. koningiopsis, and T.virens, and detect the genus of Trichoderma. Here, the rapid, simple, and reliable PCR methods were development using the species-specific primers EHarF2/EHarR2, EKoisF/EKoisR, and EVireF/EVireR to produce 253 bp (tef1 gene), 255 bp (tef1 gene), and 263 bp (tef1 gene) DNA bands to identify T. harzianum, T. koningiopsis, and T. virens, respectively. The genus-specific primers ITricF/ITricR produces a single DNA band in the range of 103–113 bp (ITS region) to distinguish Trichoderma from other genera fungi, and the size of the band is related to the species of Trichoderma. In addition, ITricF/ITricR could use for real-time PCR amplification for detection the quantity of Trichoderma spp..

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“…Based on previous studies [37][38][39], the translation elongation factor alpha 1 (Tef1) gene was selected to develop a taxon-specific real-time PCR method targeting T. reesei species. Using the Primer3 (v. 0.4.0) software, a set of primers and probes was designed, allowing for the amplification of 130 bp of the T. reesei Tef1 gene (Table 1) [40,41].…”

Section: Development and Validation Of The Real-time Pcr Tr Methodsmentioning

confidence: 99%

Development of a Taxon-Specific Real-Time Polymerase Chain Reaction Method to Detect Trichoderma reesei Contaminations in Fermentation Products

Fraiture,

Gobbo,

Papazova

et al. 2023

Fermentation

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Recently, a genetically modified microorganism (GMM) detection strategy using real-time PCR technology was developed to control fermentation products commercialized in the food and feed chain, allowing several unexpected GMM contaminations to be highlighted. Currently, only bacterial strains are targeted by this strategy. Given that fungal strains, like Trichoderma reesei, are also frequently used by the food industry to produce fermentation products, a novel real-time PCR method specific to this fungal species was developed and validated in this study to reinforce the GMM detection strategy. Designed to cover a sequence of 130 bp from the translation elongation factor alpha 1 (Tef1) gene of T. reesei, this real-time PCR method, namely TR, allows for the screening of commercial fermentation products contaminated with T. reesei, genetically modified or not, which is one of the major fungal species used as an industrial platform for the manufacturing of fermentation products. The developed real-time PCR TR method was assessed as specific and sensitive (LOD95% = eight copies). In addition, the developed real-time PCR TR method performance was confirmed to be in line with the “Minimum Performance Requirements for Analytical Methods of GMO Testing” of the European Network of GMO Laboratories. The validated real-time PCR TR method was also demonstrated to be applicable to commercial microbial fermentation products. Based on all these results, the novel real-time PCR TR method was assessed as valuable for strengthening the current GMM detection strategy regarding major fungal species used by the food industry to produce microbial fermentation products.

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A rapid, one step molecular identification of Trichoderma citrinoviride and Trichoderma reesei

Cited by 4 publications

References 11 publications

Endophytic Trichoderma citrinoviride isolated from mountain-cultivated ginseng (Panax ginseng) has great potential as a biocontrol agent against ginseng pathogens

Endophytic Trichoderma citrinoviride isolated from mountain-cultivated ginseng (Panax ginseng) has great potential as a biocontrol agent against ginseng pathogens

Development of Species/Genus specific Primers for Identification of Three Trichoderma Species and for Detection of Trichoderma Genus

Development of a Taxon-Specific Real-Time Polymerase Chain Reaction Method to Detect Trichoderma reesei Contaminations in Fermentation Products

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