A rapid, sensitive, and selective bioluminescence resonance energy transfer (BRET)-based nucleic acid sensing system

Kumar, Manoj; Zhang, Daohong; Broyles, David; Deo, Sapna K.

doi:10.1016/j.bios.2011.08.043

Cited by 40 publications

(25 citation statements)

References 29 publications

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“…This can be achieved by developing methodologies in which individual reagent-components are modified in such a way as to support self-assembly [18] of the desired light emitting constructs. These light emitting constructs often utilize fluorescent resonance energy transfer (FRET) [3, 19] or bioluminescent resonance energy transfer (BRET) [4, 20-23] mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Design and development of high bioluminescent resonance energy transfer efficiency hybrid-imaging constructs

Kumar

Kovalski

Broyles

et al. 2016

Analytical Biochemistry

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Here we describe the design and construction of an imaging construct with high bioluminescent resonance energy transfer (BRET) efficiency that is comprised of multiple quantum dots (QDs, λem 655 nm) self-assembled onto a bioluminescent protein, Renilla luciferase (Rluc). This is facilitated by the streptavidin-biotin interaction, allowing the facile formation of a hybrid-imaging-construct (HIC) comprising up to 6 QDs (acceptor) grafted onto a light emitting Rluc (donor) core. The resulting assembly of multiple acceptors surrounding a donor permits this construct to exhibit high resonance energy transfer efficiency (~64.8%). The HIC was characterized using fluorescence excitation anisotropy measurements and high resolution transmission electron microscopy. To demonstrate the application of our construct, a generation-5 (G5) polyamidoamine dendrimer (PAMAM) nanocarrier was loaded with our HIC for in vitro and in vivo imaging. We envision that this design of multiple acceptors and bioluminescent donor would lead to the development of new BRET-based systems useful in sensing, imaging, and other bioanalytical applications.

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Section: Introductionmentioning

confidence: 99%

Design and development of high bioluminescent resonance energy transfer efficiency hybrid-imaging constructs

Kumar

Kovalski

Broyles

et al. 2016

Analytical Biochemistry

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“…[1] QDs are popular RET donors due to their high quantum yield and tuneable photoluminescence (PL).The surface area of QDs allows the immobilization of multiple recognition elements, where a single QD can participate in multiple RET events [1]. Our group has paired green-emitting QDs (gQDs) and red-emitting QDs (rQDs) with various molecular dyes as RET acceptors in nucleic acid hybridization assays [2,3].While QDs are popular RET donors, their use as RET acceptors has been limited to time gated measurements [4], chemiluminescence resonance energy transfer (CRET) [5] and bioluminescence resonance energy transfer (BRET) [6]. The broad absorbance band of QDs that stretches into the UV region of the spectrum makes them susceptible to direct excitation in the process of exciting the RET donor.…”

Section: Quantum Dots In Resonance Energy Transfermentioning

confidence: 99%

Resonance Energy Transfer-Based Nucleic Acid Hybridization Assays on Paper-Based Platforms Using Emissive Nanoparticles as Donors

Doughan

Noor

Han

et al. 2017

Biosensors and Biodetection

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Abstract:Quantum dots (QDs) and upconverting nanoparticles (UCNPs) are luminescent nanoparticles (NPs) commonly used in bioassays and biosensors as resonance energy transfer (RET) donors.The narrow and tunable emissions of both QDs and UCNPs make them versatile RET donors that can be paired with a wide range of acceptors. Ratiometric signal processing that compares donor and acceptor emission in RET-based transduction offers improved precision, as it accounts for fluctuations in the absolute photoluminescence (PL) intensities of the donor and acceptor that can result from experimental and instrumental variations. Immobilizing NPs on a solid support avoids problems such as those that can arise with their aggregation in solution, and allows for facile layerby-layer assembly of the interfacial chemistry. Paper is an attractive solid support for the development of point-of-care diagnostic assays given its ubiquity, low-cost and intrinsic fluid transport by capillary action. Integration of nanomaterials with paper-based analytical devices (PADs) provides avenues to augment the analytical performance of PADs, given the unique optoelectronic properties of nanomaterials. Herein, we describe methodology for the development of PADs using QDs and UCNPs as RET donors for optical transduction of nucleic acid hybridization. Immobilization of green-emitting QDs (gQDs) on imidazole functionalized 2 cellulose paper is described for use as RET donors with Cy3 molecular dye as acceptors for the detection of SMN1 gene fragment. We also describe the covalent immobilization of blue-emitting UCNPs on aldehyde modified cellulose paper for use as RET donors with orange-emitting QDs (oQDs) as acceptors for the detection of HPRT1 gene fragment. The data described herein is acquired using an epifluorescence microscope, and can also be collected using technology such as a typical electronic camera.

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“…Although not as well-known as FRET, a new sensing mechanism known as bioluminescent resonant energy transfer has emerged [94–96]. Rather than achieving the excited donor state through electromagnetic excitation, the BRET mechanism relies on achieving electron excitation through bio-chemical reactions.…”

Section: Semiconductor Quantum Dotsmentioning

confidence: 99%

Recent Advances in the Field of Bionanotechnology: An Insight into Optoelectric Bacteriorhodopsin, Quantum Dots, and Noble Metal Nanoclusters

Knoblauch

Griep

Friedrich

2014

Sensors

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Molecular sensors and molecular electronics are a major component of a recent research area known as bionanotechnology, which merges biology with nanotechnology. This new class of biosensors and bioelectronics has been a subject of intense research over the past decade and has found application in a wide variety of fields. The unique characteristics of these biomolecular transduction systems has been utilized in applications ranging from solar cells and single-electron transistors (SETs) to fluorescent sensors capable of sensitive and selective detection of a wide variety of targets, both organic and inorganic. This review will discuss three major systems in the area of molecular sensors and electronics and their application in unique technological innovations. Firstly, the synthesis of optoelectric bacteriorhodopsin (bR) and its application in the field of molecular sensors and electronics will be discussed. Next, this article will discuss recent advances in the synthesis and application of semiconductor quantum dots (QDs). Finally, this article will conclude with a review of the new and exciting field of noble metal nanoclusters and their application in the creation of a new class of fluorescent sensors.

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A rapid, sensitive, and selective bioluminescence resonance energy transfer (BRET)-based nucleic acid sensing system

Cited by 40 publications

References 29 publications

Design and development of high bioluminescent resonance energy transfer efficiency hybrid-imaging constructs

Design and development of high bioluminescent resonance energy transfer efficiency hybrid-imaging constructs

Resonance Energy Transfer-Based Nucleic Acid Hybridization Assays on Paper-Based Platforms Using Emissive Nanoparticles as Donors

Recent Advances in the Field of Bionanotechnology: An Insight into Optoelectric Bacteriorhodopsin, Quantum Dots, and Noble Metal Nanoclusters

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