A rapid, sensitive and solvent‐less method for determination of malonaldehyde in meat by stir bar sorptive extraction coupled thermal desorption and gas chromatography/mass spectrometry with <i>in situ</i> derivatization

Ruan, Eric Dongliang; Aalhus, J. L.; Juárez, M.

doi:10.1002/rcm.7058

Cited by 18 publications

(9 citation statements)

References 25 publications

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“…The samples prepared in the above procedures were detected in the following two methods in the same manner: In the CLEIA method, 1 mL of the supernatant was blown to dryness at 30°C under nitrogen, and the residue was dissolved in 5 mL 6.4% DMF-competitive reaction buffer solution (sample diluted to a concentration in the CLEIA detection linear range), and the dissolved solution was measured by CLEIA. In the GC-MS method[ 25 – 26 ] 4 mL supernatant was blown to dryness at 30°C under nitrogen, and the residue was dissolved in 4 mL of n-hexane, and the solution was filtered through 0.22 μm organic phase membrane for GC-MS analysis.…”

Section: Methodsmentioning

confidence: 99%

The Rapid Screening of Triazophos Residues in Agricultural Products by Chemiluminescent Enzyme Immunoassay

et al. 2015

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A highly sensitive chemiluminescent enzyme immunoassay (CLEIA) method was developed in this study for efficient screening of triazophos residues in a large number of samples. Based on the maximum residue limits (MRLs) set by China and CAC for triazophos in different agro-products, the representative apple, orange, cabbage, zucchini, and rice samples were selected as spiked samples, and the triazophos at the concentrations of the MRL values were spiked to blank samples. Subsequently, the five samples with the spiked triazophos standard were measured by CLEIA 100 times, and the detection results indicated that the correction factors of the apple, orange, cabbage, zucchini, and rice were determined as 0.79, 0.66, 0.85, 0.76, and 0.91, respectively. In this experiment, 1500 real samples were detected by both the CLEIA and the GC-MS methods. With the GC-MS method, 1462 samples were identified as negative samples and 38 samples as positive samples. Based on the correction factors, the false positive rate of the CLEIA method was 0.13%, and false negative rate was 0. The results showed that the established CLEIA method could be used to screen a large number of real samples.

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Section: Methodsmentioning

confidence: 99%

The Rapid Screening of Triazophos Residues in Agricultural Products by Chemiluminescent Enzyme Immunoassay

et al. 2015

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“…SBSE and direct immersion-SPME (DI-SPME) have the same extraction principle, but it has higher analytical sensitivity (>100 times) due to the increase in extraction phase volume. Weak polarity polydimethylsiloxane (PDMS), currently the only commercialized SBSE, is more suitable for extracting derivatives than MDA or HNE (Ruan, Aalhus, & Juárez, 2014;Stopforth, Burger, Crouch, & Sandra, 2006). LPME can avoid the problems of poor repeatability between SPME fibers and fiber wear but requires precise manual operation (Fiamegos & Stalikas, 2008;Mirmoghaddam, Kaykhaii, & Hashemi, 2016).…”

Section: Other Microextraction Technologiesmentioning

confidence: 99%

Typical reactive carbonyl compounds in food products: Formation, influence on food quality, and detection methods

Zhao

et al. 2020

Comp Rev Food Sci Food Safe

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Reactive carbonyl compounds are a large group of highly reactive electrophilic compounds containing one or more carbonyl groups, which can be created by lipid oxidation both in vivo and in food. Malondialdehyde (MDA) and 4‐hydroxy‐2‐nonenel (HNE) are the two most important reactive carbonyl compounds in food. They can react with proteins and nucleic acids and cause biological damage to cells and lead to carbonyl stress. Therefore, they are regarded as representative products of lipid oxidation, toxic molecules, and biomarkers of oxidative stress. Apart from biological toxicity, they can also react with myoglobin and myofibrillar protein and further affect color, gel properties, hydrophobicity, or other properties of food. However, the effects of MDA and HNE on food qualities have not received as much attentions and it is noteworthy that the existing analytical methods for detecting MDA and HNE have a variety of limitations due to the complexity of food samples. To provide a comprehensive understanding of HNE and MDA, the formation mechanism, occurrence, and analytical methods for MDA and HNE in food matrix were summarized in this article. Emphasis is focused on formation mechanism including non‐enzymatic pathway and enzymatic pathway, and detection methods including the extraction methods, the new development of sample pre‐treatment technology and the selection of derivative reagents. Impressively, the reaction mechanism of MDA and HNE with myoglobin or myofibrillar protein is also described to explain how MDA and HNE affect food quality.

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“…Furthermore, the thick coating of sorptive media on the stir bar allows a larger phase volume to be realized (tens of microliters) compared to the SPME approach . Since the advent of this technique in the late 90s, numerous groups have employed this solventless extraction technique to detect the aromatic volatile compounds, for instance, those present in plants , beverages , and animal products with far better sensitivity and repeatability than traditional techniques. The large phase volume of these stir bars (32 μL of stir bar sorptive extraction sorptive phase as opposed to 0.6 μL in a traditional SPME fibres) further helps in absorbing a wider spectrum of organic compounds.…”

Section: Introductionmentioning

confidence: 99%

Profiling of headspace volatiles from Escherichia coli cultures using silicone‐based sorptive media and thermal desorption GC–MS

Devaraj

Pook

Swift

et al. 2018

J of Separation Science

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Headspace sorptive extraction technique using silicone based sorptive media coated stir bars is used for the first time here to extract, identify, and quantify heavy volatile organic compounds present in Escherichia coli culture headspace. Detection of infection presence is largely accomplished in laboratories through physical sampling and subsequent growth of cultures for biochemical testing. The use of volatile biomarkers released from pathogens as indicators for pathogenic presence can vastly reduce the time needed whilst improving the success rates for infection detection. To validate this, by using a contactless headspace sorptive extraction technique, the volatile compounds released from E. coli, grown in vitro, have been extracted and identified. Two different sorptive media for extracting these headspace volatiles were compared in this study and the identified volatiles were quantified. The large phase volume and wider retention of this sorptive technique compared to traditional sampling approach enabled preconcentration and collection of wider range of volatiles towards developing an extensive database of such heavy volatiles associated with E. coli. This supplements the existing data of potential bacterial markers and use of internal standards in these tests allows semi-quantitative estimation of these compounds towards the development and optimization of novel pathogen sensing devices.

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A rapid, sensitive and solvent‐less method for determination of malonaldehyde in meat by stir bar sorptive extraction coupled thermal desorption and gas chromatography/mass spectrometry with in situ derivatization

Abstract: The SBSE-TD-GC/MS method was suitable to monitor and analyze MDA in meat samples at trace levels. The simple, sensitive and solvent-less method with moderated in situ derivatization can be applied for analysis of MDA in a wide variety of foods and biological samples.

Cited by 18 publications

References 25 publications

The Rapid Screening of Triazophos Residues in Agricultural Products by Chemiluminescent Enzyme Immunoassay

The Rapid Screening of Triazophos Residues in Agricultural Products by Chemiluminescent Enzyme Immunoassay

Typical reactive carbonyl compounds in food products: Formation, influence on food quality, and detection methods

Profiling of headspace volatiles from Escherichia coli cultures using silicone‐based sorptive media and thermal desorption GC–MS

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