A rapid spectrophotometric method for determination of fluoride in silicates with the zirconyl-xylenol orange complex

Sahu, P.; Panda, J. D.; Sinha, B. C.

doi:10.1016/0039-9140(92)80178-g

Cited by 8 publications

(3 citation statements)

References 19 publications

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“…Previous studies have shown that the use of the fluoride indicator dye (zirconium-xylenol orange metal chelate) is a sensitive method for quantifying fluoride in solution (Wilson and Cooke 1966). However, factors such as zirconium polymerization, reagent concentration and interference with certain ions can affect the reproducibility, sensitivity and the utility of this method (Sahu et al 1992). To develop this colony-screening assay, we optimized the reaction conditions and incorporated the reagents into an agarose matrix (colorimetric overlay), which was poured over colonies growing on agarose growth plates.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have shown that the use of the fluoride indicator dye (zirconium‐xylenol orange metal chelate) is a sensitive method for quantifying fluoride in solution (Wilson and Cooke 1966). However, factors such as zirconium polymerization, reagent concentration and interference with certain ions can affect the reproducibility, sensitivity and the utility of this method (Sahu et al. 1992).…”

Section: Introductionmentioning

confidence: 99%

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Development of a colorimetric colony-screening assay for detection of defluorination by micro-organisms

Davis

Denman

Sly

et al. 2011

Letters in Applied Microbiology

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Aims: To develop a colorimetric colony‐screening assay to facilitate the isolation of micro‐organisms capable of defluorination. Methods and Results: A metal‐dye chelate, zirconium‐xylenol orange was used to detect fluoride ions released from a fluorinated substrate through microbial metabolism. Depolymerised zirconium reagent gave the greatest visual contrast for the presence of fluoride compared to more polymerised forms of zirconium reagent. The sensitivity of the assay was greatest when the molar ratio of depolymerised zirconium to xylenol orange was 1 : 2. Using depolymerised zirconium and xylenol orange (150 and 300 nmol l−1 respectively), the assay could detect a fluoride application spot (5 mmol l−1) containing 50 nmoles of fluoride ions. Most media constituents were well tolerated by the assay, although phosphate ions needed to be restricted to 0·1 g l−1 and some proteins digest to between 1 and 5 g l−1. A microbial enrichment culture growing on solidified medium containing 20 mmol l−1 fluoroacetate was screened using the assay, and defluorinating bacteria belonging to the genus Burkholderia isolated. Conclusions: A method was developed that is sensitive, rapid and reliable for detecting defluorination by micro‐organisms growing on solidified medium. Significance and Impact of the Study: This method can be used to facilitate the isolation of micro‐organisms capable of defluorination.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a colorimetric colony-screening assay for detection of defluorination by micro-organisms

Davis

Denman

Sly

et al. 2011

Letters in Applied Microbiology

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“…Various methods for the determination of fluoride have been reported in the literature. 4,5 The most common techniques used to determine F À1 ions are fluoride ion selective electrode (F-ISE), [6][7][8] ion chromatography (IC), [9][10][11] spectrophotometry, [12][13][14][15] molecular absorption spectrometry (MAS), [16][17][18] nuclear magnetic resonance (NMR), 19,20 gas chromatography 21 and titrimetry. [22][23][24] But most of these instrumental methods suffer from various limitations for fluoride analysis.…”

Section: Introductionmentioning

confidence: 99%

A simple UV-photolysis digestion method for the determination of fluoride in fluorine-containing drugs by ion-selective electrode and spectrophotometry techniques

et al. 2012

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A simple, effective and reliable UV photolysis digestion (UVPD) method has been developed for the determination of fluoride in pharmaceuticals containing fluorine as one of the constituents. It is based on the use of high intensity UV-irradiation in the presence of a mixture of HNO 3 and methanol. For the optimization of the UVPD procedure, three bulk drug samples namely levofloxacin, nebivolol and efavirenz, were chosen as representatives of three diverse matrices: containing single fluorine, two fluorine atoms and trifluoromethyl groups respectively. Optimization of the procedure involves an initial reaction with nitric acid followed by a UVPD approach for complete mineralization of the sample i.e., conversion of bound fluorine to free fluoride ion followed by determination by fluoride ionselective electrode (F-ISE) and spectrophotometry based methods. Parameters affecting the UVPD method, such as acid concentration, digestion time and sample weight, were optimized to obtain the quantitative recovery (>95%) of fluoride. Quantitative recovery of the fluorine in the samples could be achieved within 20-30 min of UV exposure time for a 50-mg sample. Total digestion of the drug samples was carried out by a closed microwave digestion system and the recoveries of the fluoride were found to be low (<70%) demonstrating the capabilities of the UVPD procedure. The proposed UVPD method was successfully applied to various commercially available fluorine containing drug samples.

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Zirconium gallocyanin MS complex as a highly selective reagent for the spectophotometric determination of fluoride

Petrenko

Marchenko

Vasil'ev

2021

Microchemical Journal

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A rapid spectrophotometric method for determination of fluoride in silicates with the zirconyl-xylenol orange complex

Cited by 8 publications

References 19 publications

Development of a colorimetric colony-screening assay for detection of defluorination by micro-organisms

Development of a colorimetric colony-screening assay for detection of defluorination by micro-organisms

A simple UV-photolysis digestion method for the determination of fluoride in fluorine-containing drugs by ion-selective electrode and spectrophotometry techniques

Zirconium gallocyanin MS complex as a highly selective reagent for the spectophotometric determination of fluoride

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