A Rapid Test for Biodegradability of PVC Film by Pseudomonads

Sharpe, A. N.; Woodrow, M. N.

doi:10.1111/j.1365-2672.1971.tb02308.x

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…Cell death was evaluated by ammonia concentrations in the samples (Sharpe & Woodrow, 1971). This was done by removing 50.0 μL aliquots of the mixtures in which the biodegradation occurred and adding 3.0 mL phenol reagent (11.68 mL phenol and 0.0625 g sodium nitroprusside (97.5%, Merck), 3.0 mL sodium hypochlorite reagent (6.25 g NaOH [97%, Merck]) and 3.67 mL sodium hypochlorite (98%, Merck).…”

Section: Evaluation Of Cell Death By Ammonia Concentrationsmentioning

confidence: 99%

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Evaluation of the biodegradation of phthalic contaminants originated from polyvinyl chloride commercial films

Luzia¹,

Sarrouh²,

Lofrano³

2020

Acta Sci Technol

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The excessive use of phthalates (PHEs) plasticizers has promoted a serious environmental problem. This study evaluated the biodegradation of PHEs from polyvinyl chloride (PVC) film, aiming to mitigate damage to the environment. Therefore, 23 microorganisms were prospected and isolated from the Serra do Ouro Branco State Park (Minas Gerais, Brazil), where screening was carried out by growth in enriched microbial medium and esterase-activity was monitored by diffusion in solid agar. Three microorganisms were selected. Bacterium (I) presented the highest esterase activity, as evidenced in the diffusion tests in solid medium. Further, evidence of this is given by the biodegradation of PHEs into phthalic acid, because changes the acid concentration from 0 to 3.10 ± 0.07 mol L-1 and 28 % mass reduction of PVC film after 24 days. Furthermore, after six days of biodegradation the number of viable cells and ammonia concentration showed no significant change. This indicates that the survival of the Bacterium (I) cells is independent of ammonia consumption, but rather depends on PHEs consumption. The detection of the organic functions of -COOH and –COOR proved the biodegradation of PHEs by Bacterium (I), thus demonstrating its potential to be used in bioremediation of soils and rivers from PHEs contaminants.

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Section: Evaluation Of Cell Death By Ammonia Concentrationsmentioning

confidence: 99%

“…The samples were then analyzed in a UV/Vis spectrophotometer at 640 nm (Biospectro SP-220 Spectrophotometer) using a glass cuvette. The concentration of ammonia produced by bacteria was determined through a standard curve of ammonia, previously constructed (Sharpe & Woodrow, 1971).…”

Section: Evaluation Of Cell Death By Ammonia Concentrationsmentioning

confidence: 99%

Evaluation of the biodegradation of phthalic contaminants originated from polyvinyl chloride commercial films

Luzia¹,

Sarrouh²,

Lofrano³

2020

Acta Sci Technol

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“…Besides supplying essential nutrients, such complex nitrogenous sources also influence adsorption (Whitaker 1952) and thus the measured enzyme activity. However, an excess of yeast extract results in its eventual deamination and utilization as a carbon source; indeed, the level of ammonia attained has been proposed as a quantitative measure of carbohydrate availability (Sharpe and Woodrow 1972).…”

Section: Discussionmentioning

confidence: 99%

Influence of Carbohydrate Accessibility on Cellulase Production by Lenzites trabea and Polyporus versicolor

Ma¹,

Stranks²

1974

Aust. Jnl. Of Bio. Sci.

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Cellulase and hemicellulase, both free and bound to cellulose, were produced in shake cultures of L. trabea and P. versicolor grown on ball-milled aspen (Populus tremuloides) or filter paper. Most of the enzyme was initially bound to cellulose plus mycelium and could not be readily dislodged, even by ultrasonic disintegration. Proportionally more enzyme could be obtained in the supernatant by reducing the cellulose concentration in the medium. No correlation was observed between the amount of growth and the amount of enzyme production. Rather, enzyme production was shown to be influenced by regulation of the growth rate through the use of cellulosic substrates of varying ease of accessibility and also when cultures were continuously supplied with glucose at a slow rate.

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