A rare monoclonal antibody discovery based on indirect competitive screening of a single hapten-specific rabbit antibody secreting cell

Abstract

Rare antibody that is able to tolerate physio-chemical factors is preferred and highly demanded in diagnosis and therapy. Rabbit monoclonal antibodies (RmAbs) are distinguished owing to their high affinity and...

Response to Comments on “Monoclonal Antibody Discovery Based on Precise Selection of Single Transgenic Hybridomas with an On-Cell-Surface and Antigen-Specific Anchor”

“…To achieve efficient and precise screening of hapten-specific antibody-secreting cells, our work in 2022 proposed an indirect competitive screening method in nanowells, named CSMN, that conducts a completive ELISA in a nanowell to specifically select the hapten-specific antibody-secreting cells, which is an efficient platform for hapten-specific antibody production (Figure ). We have successfully produced rabbit monoclonal antibodies with high affinity and specificity based on the CMSN …”