A rare secoiridoid monoterpene and a xanthone from Tachia grandiflora Maguire &amp; Weaver

Pohlit, Adrian Martin; Santos, Elba Vieira Mustafa dos; Silva, Tatiane Cristina Mesquita da; Morais, Sabrina Kelly Reis de; Nunomura, Sergio Massayoshi; Struwe, Lena

doi:10.1016/j.bse.2012.05.012

Cited by 6 publications

(5 citation statements)

References 6 publications

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“…From the active root and leaf chloroform fractions of T. grandiflora the antiplasmodial tetra-oxygenated xanthone decussatin (1) and the rare seco-iridoid aglycone amplexine (2) were, respectively, isolated ( Pohlit et al 2012). Tetra-oxygenated xanthones like 1 are commonly isolated from gentians and seco-iridoids are also isolated from gentians.…”

Section: Discussionmentioning

confidence: 99%

“…Fraction 8 contained yellowish white crystals in the form of small plates or needles of 1 (24 mg). These procedures were partially described in Pohlit et al (2012).…”

Section: Methodsmentioning

confidence: 99%

“…Three fractions were obtained and fraction 3 (46 mg) was further purified by preparative TLC to yield 2 (25 mg) as a clear liquid [R F = 0.60, ethyl acetate-methanol (2:1)] which, was identified as the secoiridoid monoterpene amplexine (or djalonenol) based on its spectral properties. This isolation procedure was partially described in Pohlit et al (2012).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we isolated the tetra-oxygenated xanthone decussatin (1) and a rare seco-iridoid monoterpene aglycone, djalonenol (amplexine) (2), from T. grandiflora ( Pohlit et al 2012). In the present work, the in vitro and in vivo antiplasmodial activity and cytoxicity of the extracts, fractions and chemical components of the leaves and roots of the central Amazonian caferana plant, T. grandiflora , were investigated.…”

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In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the Amazonian plant Tachia grandiflora (Gentianaceae)

Silva

Lima

Vasconcellos

et al. 2013

Mem. Inst. Oswaldo Cruz

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Tachia sp. are used as antimalarials in the Amazon Region and in vivo antimalarial activity of a Tachia sp. has been previously reported. Tachia grandiflora Maguire and Weaver is an Amazonian antimalarial plant and herein its cytotoxicity and antimalarial activity were investigated. Spectral analysis of the tetraoxygenated xanthone decussatin and the iridoid aglyone amplexine isolated, respectively, from the chloroform fractions of root methanol and leaf ethanol extracts was performed. In vitro inhibition of the growth of Plasmodium falciparum Welch was evaluated using optical microscopy on blood smears. Crude extracts of leaves and roots were inactive in vitro. However, chloroform fractions of the root and leaf extracts [half-maximal inhibitory concentration (IC50) = 10.5 and 35.8 µg/mL, respectively] and amplexine (IC50= 7.1 µg/mL) were active in vitro. Extracts and fractions were not toxic to type MRC-5 human fibroblasts (IC50> 50 µg/mL). Water extracts of the roots of T. grandiflora administered by mouth were the most active extracts in the Peters 4-day suppression test in Plasmodium berghei-infected mice. At 500 mg/kg/day, these extracts exhibited 45-59% inhibition five to seven days after infection. T. grandiflora infusions, fractions and isolated substance have potential as antimalarials.

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Section: Discussionmentioning

confidence: 99%

“…Fraction 8 contained yellowish white crystals in the form of small plates or needles of 1 (24 mg). These procedures were partially described in Pohlit et al (2012).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the Amazonian plant Tachia grandiflora (Gentianaceae)

Silva

Lima

Vasconcellos

et al. 2013

Mem. Inst. Oswaldo Cruz

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“…(Gentianaceae, Helieae) is a widespread, but not abundant, component of the understory of neotropical rainforests, occurring throughout the Amazon Basin, Andes and the Guiana Shield of South America, and southernmost Mesoamerica. The genus comprises 13 species, some of which are known as anti-malarial remedies in Guyana and Brazil (Carvalho et al 1991;Pohlit et al 2012). These shrubs or small trees are characteristic in their axillary, usually solitary and sometimes large flowers on unbranched stems.…”

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confidence: 99%