A rat model of cardiopulmonary bypass with cardioplegic arrest and hemodynamic assessment by conductance catheter technique

Günzinger, Ralf; Wildhirt, Stephen M.; Schad, H.; Heimisch, W.; Mendler, N.; Grammer, Joachim B.; Lange, Rüdiger; Bauernschmitt, Robert

doi:10.1007/s00395-007-0668-x

Cited by 22 publications

(25 citation statements)

References 29 publications

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“…Few studies reported a truly relevant model of complete CPB with low priming volume and without blood donor rats. 15,16,19,20 However, in these studies, data are not easily comparable due to different, mostly home-made, oxygenators.…”

Section: Discussionmentioning

confidence: 94%

“…Few studies in the literature report on truly clinically relevant models of complete CPB detailing fluid flow, surface area, and priming volume of the circuitry. 15,16 The present work outlines development and validation of a complex small animal model of CPB with a new miniaturized hollow fiber oxygenator mimicking current clinical standards. This model is reproducible and may be the starting point for study of various patho-physiologic effects related to CPB, myocardial protection, and systemic inflammation in vivo.…”

mentioning

confidence: 99%

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Validation of a Rat Model of Cardiopulmonary Bypass With a New Miniaturized Hollow Fiber Oxygenator

Cresce¹,

Walpoth²,

Mugnai³

et al. 2008

ASAIO Journal

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Cardiopulmonary bypass (CPB) is an essential component of cardiac surgery, with still unknown device/patient interactions. To evaluate the response of CPB to hemodynamic, biochemical, inflammatory, as well as thermo-pharmacodynamic interactions, a novel miniaturized oxygenator with controlled and standardized specifications has been developed together with an improved surgical central cannulation technique. A hollow-fiber small priming volume (6.3 ml) oxygenator was manufactured according to specifications resulting from engineering, heart surgery and perfusion expertise (Dideco-Sorin Group, Italy) with the following characteristics: Gas Exchange Surface--450 cm2, and Heat Exchange Surface--16 cm2. The oxygenator was tested in vitro and in vivo in five anesthetized, ventilated, open-chest rats using a miniaturized roller pump. Pressures were monitored in the animal before and after the oxygenator. Central venous cannulation through the superior vena cava and aortic cannulation through the carotid artery were used. In vitro: blood oxygenation increased 10-fold (from room air to 100% O2) and PCO2 removal was 2.5-fold. In vivo: CPB was performed without blood prime for 90 minutes (no ventilation) maintaining stable hemodynamics. A maximal blood flow rate of 124 ml/min/kg was obtained. Arterio-venous PO2 gradients were 10-fold (O2 100%) with only small variations when changing blood flow rates. This new, standardized and miniaturized hollow fiber oxygenator, new cannulation technique and CPB circuit achieved optimal gas transfer with small asanguinous priming volumes. This study opens new potentials for various CPB-related study protocols in the small animal.

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Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

Validation of a Rat Model of Cardiopulmonary Bypass With a New Miniaturized Hollow Fiber Oxygenator

Cresce¹,

Walpoth²,

Mugnai³

et al. 2008

ASAIO Journal

View full text Add to dashboard Cite

show abstract

“…11 Extracorporeal circulation resulted in a marked increase in proinflammatory cytokines (IL-6 and TNF-a). 11,25 This is of great importance, because proinflammatory cytokines promote the release of MMP-2 and MMP-9. 26 Mean  SD, p < 0.05: *pre-ECC vs. post-ECC (Wilcoxon), †vs.…”

Section: Discussionmentioning

confidence: 99%

“…The complete surgical procedure and the complete development and design of the perfusion circuit were previously described in detail. 11 …”

Section: Animals Surgical Procedure and Perfusion Circuitmentioning

confidence: 97%

“…10 This increase in proinflammatory cytokines, such as tumor necrosis factor (TNF)-a and interleukin (IL)-6, has also been shown in our recently developed in vivo model of rat CPB. 11 Proinflammatory cytokines promote mediators of inflammation such as nitric oxide synthase and MMPs and contribute to myocardial dysfunction that commonly occurs after CPB. 12 It has been shown that infusion of a nitric oxide scavenger was associated with improved hemodynamics and decreased myocardial MMP activities after CPB.…”

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confidence: 99%

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Role of Metalloproteinases and Tissue Inhibitors of Metalloproteinases During Cardiopulmonary Bypass in Rats

Guenzinger

Lahm²,

Wottke³

et al. 2012

ASAIO Journal

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Matrix metalloproteinases (MMPs) and the tissue inhibitors of matrix metalloproteinases (TIMPs) regulate matrix remodeling in the heart. Changes in synthesis and release of MMPs and TIMPs are observed after extracorporeal circulation (ECC). Thus, MMPs and TIMPs are supposed to be involved in ECC-mediated cardiac dysfunction. The aim was to examine the role of MMPs and TIMPs in ECC-mediated cardiac dysfunction. Extracorporeal circulation was instituted in rats for 60 min at a flow rate of 120 ml/kg/min. Three groups (n = 10) were studied: group CAO: 60 min ECC without aortic cross-clamping, group CAC: 60 min ECC including 30 min aortic cross-clamping (crystalloid Inzolen(®) cardioplegia), and group CAB: 60 min ECC including 30 min aortic cross-clamping (blood cardioplegia). Left ventricular (LV) function was measured with conductance catheter. Matrix metalloproteinase-activity was determined by zymography and TIMP activity was determined by reverse zymography. Gene expression of MMPs and TIMPs was determined by real-time polymerase chain reaction. Sixty minutes after weaning from bypass, there was a preserved LV function in the CAO and CAB group and an impaired LV function in the CAC group. We observed an increased myocardial activity and an increased myocardial messenger RNA expression of MMP-2, MMP-9, TIMP-1, and TIMP-4 in all ECC groups, when compared with sham animals. With regard to enzyme activity, there was an imbalance of MMP/TIMP ratio leading to an increased activity of MMP in the CAC group. In terms of gene expression, there was an imbalance of MMP-2/TIMP-4 ratio leading to an increased expression of MMP-2 in the CAC group. MMP-2 contributes to myocardial reperfusion injury in this in vivo model of ECC with cardioplegic arrest.

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miRNA-124-3p targeting of LPIN1 attenuates inflammation and apoptosis in aged male rats cardiopulmonary bypass model of perioperative neurocognitive disorders

Han

Xiao

et al. 2021

Experimental Gerontology

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A rat model of cardiopulmonary bypass with cardioplegic arrest and hemodynamic assessment by conductance catheter technique

Abstract: We have developed a rat CPB with mild hypothermic cardioplegic arrest. This rodent model is suitable to study clinically relevant problems related to CPB,myocardial protection and systemic inflammation.

Cited by 22 publications

References 29 publications

Validation of a Rat Model of Cardiopulmonary Bypass With a New Miniaturized Hollow Fiber Oxygenator

Validation of a Rat Model of Cardiopulmonary Bypass With a New Miniaturized Hollow Fiber Oxygenator

Role of Metalloproteinases and Tissue Inhibitors of Metalloproteinases During Cardiopulmonary Bypass in Rats

miRNA-124-3p targeting of LPIN1 attenuates inflammation and apoptosis in aged male rats cardiopulmonary bypass model of perioperative neurocognitive disorders

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