A rat model of knee osteoarthritis suitable for electroacupuncture study

Ma, Yongyuan; Guo, Haiyun; Bai, Feihu; Zhang, Ming; Yang, Liu; Deng, Jiao; Xiong, Lize

doi:10.1538/expanim.17-0142

Cited by 21 publications

(23 citation statements)

References 32 publications

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“…Notably, the reduced PWT remained longer compared with that in the decrease in the weight bearing of the ipsilateral paw (21 days vs. 7 days, respectively). In contrast to this finding, a previous study reported that MIA, at a dose of 1 or 3 mg, induced a significant reduction in the weight bearing of the ipsilateral hind limb from days 3-28 compared with control rats (14). The difference in the duration between the reduced PWT and the weight bearing may be attributed to various potential mechanisms, such as the chondrocyte degeneration, that has been observed at days 1-7 post MIA injection (24) or a potential transient inflammation that has been indicated at days 1-4 post MIA injection (25).…”

Section: Discussioncontrasting

confidence: 70%

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Impairment in locomotor activity as an objective measure of pain and analgesia in a rat model of osteoarthritis

et al. 2020

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A major problem with current animal models of pain is their lack of face validity and their vulnerability for false positive results. The present study evaluated the efficacy of the open field locomotor system, as an objective measure of pain-related behavior and analgesic efficacy in rodents. Adult, male, Sprague-Dawley rats (180-250 g) received intra-articular injections of monoiodoacetate (MIA; 1 mg) in the left knee joint. Mechanical allodynia using von Frey filaments, the weight bearing difference test and the open field locomotor activity test were performed every other day for 21 days, following the MIA injection. The antinociceptive effects of ibuprofen (50 and 100 mg/kg) on the MIA-induced nociception were also evaluated. MIA induced a significant reduction in the paw withdrawal threshold (PWT) and a significant alteration in the weight bearing difference compared with control rats. Similarly, MIA induced a significant reduction in locomotor activity, with respect to X total counts, that represent the overall locomotor activity in the horizontal plane, and X ambulatory counts, which in turn represent small scale movements, such as scratching and grooming, and lastly, Z total counts, that represent rearing or standing. Both doses of ibuprofen resulted in a significant reversal of the MIA-induced alterations in PWT and weight bearing difference. Furthermore, the two doses of ibuprofen resulted in a significant reversal of the MIA-induced reduction in locomotor activity, with respect to X ambulatory counts, but not Z total counts. Only the higher dose of ibuprofen reversed the X total counts. The open field locomotor system may successfully be used to predict the analgesic efficacy of compounds in models of joint inflammation and osteoarthritis.

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Section: Discussioncontrasting

confidence: 70%

“…For the induction of OA, the rats received intra-articular injections of MIA (1 mg in 50 µl saline) in the left knee joint. The selection of the MIA dose was based on previous studies, in which MIA exhibited significant effects in different assays (14,15). This procedure was performed under transient isoflurane (3%) inhalation anesthesia.…”

Section: Animalsmentioning

confidence: 99%

Impairment in locomotor activity as an objective measure of pain and analgesia in a rat model of osteoarthritis

et al. 2020

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“…35 Ma et al also determined that early electroacupuncture (EA) intervention can significantly reduce joint pain and pathological lesions induced by MIA injection compared with mid-and late-term EA interventions. 36 Interestingly, a study reported that a 808 nm near infrared laser generated the antianalgesic effect from 5 to 30 min after treatment and vanished after 60 min. 37 Kim and Kim conducted a 3-week treatment using an 850 nm galliumaluminum-arsenide laser, resulting in the weight-bearing difference, PWMT, and PWL significantly reversed, and the serum levels of TNF-a, IL-1b, and IL-6 also decreased in a MIA-induced KOA model.…”

Section: Effects Of Laser Moxibustion On Mia-induced Koamentioning

confidence: 99%

The Effects of Laser Moxibustion on Knee Osteoarthritis Pain in Rats

Wei

et al. 2020

Photobiomodulation, Photomedicine, and Laser Surgery

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Background: Although chronic pain affects the quality of life of patients with osteoarthritis, current medical treatments are either ineffective or have long-term side effects. Recently, low-intensity laser irradiation of corresponding acupoints was demonstrated to alleviate pain. Objective: The aim of the present study was to investigate the effects of 10.6 lm laser moxibustion on a monosodium iodoacetate (MIA)-induced knee osteoarthritis pain model. Methods: Thirty-two rats were randomly assigned to four groups: Saline, MIA, MIA+Laser, and MIA+Sham Laser. The 10.6 lm laser was used to irradiate the ST35 for 10 min once a day for a total of seven applications. The paw withdrawal mechanical threshold and weight-bearing difference were performed to evaluate the analgesic effects of laser moxibustion. At the end of the experiment on days 28, the joint histology, the levels of metalloproteinases-13 (MMP-13) in the cartilage, and TNF-a, IL-1b, and IL-6 in the synovial membrane were measured to determine the chondroprotection and anti-inflammatory effect of laser moxibustion. Results: Early laser moxibustion significantly reversed the MIA-induced mechanical hyperalgesia and weightbearing difference, especially on the 28th day (p < 0.001). Moreover, laser moxibustion prevented the articular pathological lesions and cartilage destruction on days 28 (p < 0.01). Remarkably, the levels of cartilage MMP-13, and synovial TNF-a, IL-1b, and IL-6 also decreased on day 28 (p < 0.05) after the early treatment of laser moxibustion. Conclusions: 10.6 lm laser moxibustion may have long-lasting analgesic, anti-inflammatory, and chondroprotection effects, suggesting that it may emerge as a potential therapeutic strategy for the chronic pain treatment of osteoarthritis.

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“…The rats in group M were subjected to KOA establishment without any subsequent treatment, simulating the natural recovery of KOA without any treatment. For the MS group, according to Ma et al [ 13 ], the location of the treatment site is shown in Figure 1 , where Dubi (ST35) is located in the depression outside the patellar ligament and Zusanli (ST36) is located at about 5 mm inferior of the capitulum fibulae and posterior-lateral to the hind-limb knee joint [ 14 ]. After KOA was established in the rats, the lit moxibustion strip (diameter 7 mm, length 12 cm) was placed 1-2 cm away from the treatment acupoint, the skin temperature “of” the acupoint was maintained at 43 ± 1°C, and the treatment lasted for 30 min [ 10 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Moxibustion on the Intestinal Flora of Rats with Knee Osteoarthritis Induced by Monosodium Iodoacetate

Chen

Jia

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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In this study, a knee osteoarthritis (KOA) rat model induced by monosodium iodoacetate (MIA) was used to study the effect of moxibustion on improving knee cartilage damage and its effect on the intestinal flora. The experimental rats were divided into the normal group (N), model group (M), moxibustion treatment group (MS), and diclofenac sodium treatment group (DS). After 4 weeks, cartilage pathological damage in the knee joint was evaluated using hematoxylin-eosin and safranin O-fast green staining analysis. ELISAs and Western blots were used to detect the expression levels of IL-1β and TNF-α in the serum and cartilage, respectively. The total DNA of the fecal samples was extracted and subjected to high-throughput sequencing of the V3-V4 region of the 16S rRNA gene to analyze the changes in the intestinal flora. In the model group, the cartilage was obviously damaged, the expression levels of IL-1β and TNF-α in the serum and cartilage were increased, and the abundance and diversity of the intestinal flora were decreased. Moxibustion treatment significantly improved the cartilage damage and reduced the concentration of inflammatory factors in the serum and cartilage. The high-throughput sequencing results showed that compared to the model group, the moxibustion treatment regulated some specific species in the intestinal microorganisms rather than the α diversity. In conclusion, our findings suggest that moxibustion treatment may work through two aspects in rats. On one hand, it directly acts on knee cartilage to promote repair, and on the other hand, it regulates the composition of the intestinal flora and reduces the production of inflammatory factors.

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A rat model of knee osteoarthritis suitable for electroacupuncture study

Cited by 21 publications

References 32 publications

Impairment in locomotor activity as an objective measure of pain and analgesia in a rat model of osteoarthritis

Impairment in locomotor activity as an objective measure of pain and analgesia in a rat model of osteoarthritis

The Effects of Laser Moxibustion on Knee Osteoarthritis Pain in Rats

Effect of Moxibustion on the Intestinal Flora of Rats with Knee Osteoarthritis Induced by Monosodium Iodoacetate

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