A ratiometric electrochemical biosensor for sensitive detection of Hg 2+ based on thymine–Hg 2+ –thymine structure

Ratiometric electrochemical detection of Pd•••π interactions: application towards electrochemical molecular logic gates

“…Ratiometric electrochemical sensing can be achieved through either tagging both termini of nucleic acid capture probes bound to electrode surfaces with redox labels that have different oxidation potentials (29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41), manipulating host-guest or metal binding interactions on electrode surfaces with electrochemically distinguishable redox probes (42)(43)(44), or the use of redox-active probes which demonstrate a unique shift in oxidation potential selectively in the presence of the analyte (45)(46). For analytes that display target catalysis as a means of amplification within diagnostic assays, we have found the latter offers a number of benefits, such as improved reaction kinetics, since probes are not surface bound, and ease of analysis, as electrodes do not require modification prior to detection (47)(48).…”

Highly sensitive electrochemical detection of mercury (II) via single ion-induced three-way junction of DNA

“…Among these sensors, the "one-step" detection strategies have attracted considerable attentions [3,4,11,[19][20][21]. They can produce the "on" or "off" switch signal through the formation of T-Hg 2+ -T base pairs.…”

Smart protein biogate as a mediator to regulate competitive host-guest interaction for sensitive ratiometric electrochemical assay of prion

“…Recently, ratiometric assays, including ratiometric electrochemical 24 25 26 27 28 and ratiometric electrochemiluminescent 29 30 methods, have attracted growing attentions. The ratiometric electrochemiluminescent assay, based on dual-potential electrochemiluminescence (ECL) measurement, needs to create ECL report units with two emitting states that have the potential-dependent properties upon the substrate concentration, while the ratiometric electrochemical assay needs to choose two redox probes which can be electrochemically oxidized/reduced at different potentials.…”

“…Based on the ratio between the oxidation peak current of MB ( I MB ) and that of FCA ( I FCA ), prion was detected sensitively and selectively. In comparison with other reported ratiometric electrochemical biosensors that usually needed one multi-labeled DNA strand or at least two single-labeled DNA strands 24 25 26 27 28 , we just used only one single-labeled target aptamer to construct the ratiometric electrochemical aptasensor, which can potentially lower the cost and simplify the ratiometric E-AB sensor. Moreover, this strategy could have promising application in the detection of a wide range of analytes by changing the corresponding specific aptamers.…”