A Ratiometric Fluorescence Probe for Selective Detection of ex vivo Methylglyoxal in Diabetic Mice

Abstract

Accurate monitoring of methylglyoxal (MGO) at cell and living level was crucial to reveal its role in the pathogenesis of diabetes since MGO was closely related to diabetes. Herein, a ratiometric fluorescence strategy was constructed based on the capture probe 2,3‐diaminonaphthalene (DAN) for the specific detection of MGO. Compared to the fluorescent probes with a single emission wavelength, the ratiometric mode by monitoring two emissions can effectively avoid the interference from the biological background, and provided additional self‐calibration ability, which can realize accurate detection of MGO. The proposed method showed a good linear relationship in the range of 0–75 μ m for MGO detection, and the limit of detection was 0.33 μ m . DAN responded to MGO with good specificity and was successfully applied for detecting the ex vivo MGO level in plasma of KK−Ay mice as a type II diabetes model. Besides, the prepared DAN test strip can be visualized for rapid semi‐quantitative analysis of MGO using the naked eye. Furthermore, human skin fibroblasts and HeLa cells were utilized for exogenous MGO imaging, and ex vivo MGO imaging was performed on tissues of KK−Ay mice. All results indicated that the DAN‐based ratiometric fluorescence probe can be used as a potential method to detect the level of MGO, thus enabling indications for the occurrence of diabetes and its complications.

Photo-Enhanced Chemo-Transistor Platform for Ultrasensitive Assay of Small Molecules

“…MGO, produced during the glycolysis process in living systems, has a notable connection with diabetes and is regarded as an emerging biomarker for diabetes and its complications. , It has been revealed that the level of MGO exhibits over 3-fold increase in the serum of diabetic mice , and up to 10-fold increase in diabetic complication regions. − In order to evaluate the sensing capability in real-world applications, we test 16 serum samples from healthy mice (H1–H8) and diabetic mice (D1–D8) by the photo-enhanced chemo-transistor (Figure a). In the dark, the chemo-transistors hardly distinguish the positive sample from the negative sample, yielding a difference in the Δ V Dirac response smaller than 3 mV (Figure b).…”

Acid-promoted fluorescent probe for monitoring endogenous methylglyoxal in tumors and gastritis

“…[1][2][3] As a highly active molecule, MGO can react with proteins, DNA, and lipids to form advanced glycation end products, resulting in irreversible cell structural damage and dysfunction, which is closely associated with pathologies of diverse human diseases including obesity, Alzheimer's disease, cardiovascular disease, and diabetes. [4][5][6][7][8][9][10] The detoxification method of MGO mainly relies on glyoxalase 1 and glyoxalase 2, which can convert MGO into non-toxic D-lactic acid to regulate the levels of MGO in cells. [11][12][13][14] Indeed, elevated levels of MGO have been observed in cancer cells due to the aerobic glycolysis pathway and are involved in tumor progression.…”