A ratiometric fluorescent probe for detecting the endogenous biological signaling molecule superoxide anion and bioimaging during tumor treatment

Wang, Yue; Wang, Xiaoyan; Zhang, Li; Huang, Yan; Bi, Liyan; Lv, Changjun; Chen, Lingxin

doi:10.1039/c9tb02453k

Cited by 21 publications

(7 citation statements)

References 59 publications

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“…•− , the triflate unit is rapidly removed, allowing effective recovery of fluorescence (Scheme 2). 51,52 Meanwhile, the high-resolution mass spectrometry (HRMS) study has validated and further proven the response mechanism between NIR-FP and O 2…”

Section: Rational Design Of the Probe Nir-fp This Research Aimed To D...mentioning

confidence: 93%

“…In recent decades, a variety of techniques have been established to detect O 2 •– . However, most of these detection techniques are complex and time-consuming. − Recently, fluorescence imaging has garnered great interest due to its noninvasiveness, high sensitivity, and high spatial–temporal. − Particularly, near-infrared (NIR) fluorescence tracing is progressively supplanting original fluorescence imaging as a preferred method for selective and in situ optical monitoring of biomolecules due to its lower interference from the background, higher sensitivity, and signal-to-noise ratio. − Although several fluorescent probes for monitoring O 2 •– in biological systems have been developed, − until now there has been no desirable NIR fluorescent probe for effective detection of the level concentration of brain O 2 •– in ferroptosis-mediated epilepsy (Table S2). Therefore, there is an urgent need to develop a NIR fluorescence probe for assessing the role of O 2 •– in ferroptosis-mediated epilepsy, which could not only be beneficial to elaborate the potential relationship between O 2 •– and epilepsy but also provide a feasible direction for discovering potential therapeutic targets of epilepsy.…”

Section: Introductionmentioning

confidence: 99%

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Novel Near-Infrared Fluorescence Probe for Bioimaging and Evaluating Superoxide Anion Fluctuations in Ferroptosis-Mediated Epilepsy

et al. 2023

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Ferroptosis is an iron-regulated, caspase-mediated pathway of cell death that is associated with the excessive aggregation of lipid-reactive oxygen species and is extensively involved in the evolution of many diseases, including epilepsy. The superoxide anion (O2 •–), as the primary precursor of ROS, is closely related to ferroptosis-mediated epilepsy. Therefore, it is crucial to establish a highly effective and convenient method for the real-time dynamic monitoring of O2 •– during the ferroptosis process in epilepsy for the diagnosis and therapy of ferroptosis-mediated epilepsy. Nevertheless, no probes for detecting O2 •– in ferroptosis-mediated epilepsy have been reported. Herein, we systematically conceptualized and developed a novel near-infrared (NIR) fluorescence probe, NIR-FP, for accurately tracking the fluctuation of O2 •– in ferroptosis-mediated epilepsy. The probe showed exceptional sensitivity and outstanding selectivity toward O2 •–. In addition, the probe has been utilized effectively to bioimage and evaluate endogenous O2 •– variations in three types of ferroptosis-mediated epilepsy models (the kainic acid-induced chronic epilepsy model, the pentylenetetrazole-induced acute epilepsy model, and the pilocarpine-induced status epilepticus model). The above applications illustrated that NIR-FP could serve as a reliable and suitable tool for guiding the accurate diagnosis and therapy of ferroptosis-mediated epilepsy.

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Section: Rational Design Of the Probe Nir-fp This Research Aimed To D...mentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Novel Near-Infrared Fluorescence Probe for Bioimaging and Evaluating Superoxide Anion Fluctuations in Ferroptosis-Mediated Epilepsy

et al. 2023

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“…The interest in cancer research stems from the success in determining the different O •− 2 concentrations in normal and cancerous tissues by using a fluorescent probe, with cancer cells showing higher levels of O •− 2 than healthy cells [43,67]. For example, Wang et al [68] investigated the effect of O •− 2 on tumor treatment using a two-photon fluorescent probe in combination with triflate as the sensing response unit (Figure 2b). The detection of O •− 2 was successfully achieved in four minutes without any disturbance from surrounding interfering species, confirming a rapid detection.…”

Section: Fluorescence Methods For Superoxide Radical (O •−mentioning

confidence: 99%

Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications

et al. 2021

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Reactive oxygen species (ROS) have been found in plants, mammals, and natural environmental processes. The presence of ROS in mammals has been linked to the development of severe diseases, such as diabetes, cancer, tumors, and several neurodegenerative conditions. The most common ROS involved in human health are superoxide (O2•−), hydrogen peroxide (H2O2), and hydroxyl radicals (•OH). Organic and inorganic molecules have been integrated with various methods to detect and monitor ROS for understanding the effect of their presence and concentration on diseases caused by oxidative stress. Among several techniques, fluorescence and electrochemical methods have been recently developed and employed for the detection of ROS. This literature review intends to critically discuss the development of these techniques to date, as well as their application for in vitro and in vivo ROS detection regarding free-radical-related diseases. Moreover, important insights into and further steps for using fluorescence and electrochemical methods in the detection of ROS are presented.

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“…Chen et al report a ratiometric fluorescence probe TP-Tfs (λ ex /λ em = 400/520 nm and 450/580 nm) for O 2 •– detection in cells and in vivo during the process of tumor treatment . TP-Tfs is comprised of a TP fluorophore indole derivative and a triflate as a response unit for O 2 •– measurement.…”

Section: Nir and Tp Fluorescence Imaging Of Ros In The Tmementioning

confidence: 99%

Deep-Tissue Fluorescence Imaging Study of Reactive Oxygen Species in a Tumor Microenvironment

Mao

Wang

et al. 2021

Anal. Chem.

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Tumor microenvironment (TME) is the survival environment for tumor cells to proliferate and metastasize in deep tissue. TME contains tumor cells, immune cells, stromal cells and a variety of active molecules including reactive oxygen species (ROS). Inside the TME, ROS regulate the oxidation–reduction (redox) homeostasis and promote oxidative stress. Due to the rapid proliferation ability and specific metabolic patterns of the TME, ROS pervade virtually all complex physiological processes and play irreplaceable roles in protein modification, signal transduction, metabolism, and energy production in various tumors. Therefore, measurements of the dynamically, multicomponent simultaneous changes of ROS in the TME are of great significance to reveal the detailed proliferation and metastasis mechanisms of the tumor. Near-infrared (NIR) and two-photon (TP) fluorescence imaging techniques possess real-time, dynamic, highly sensitive, and highly signal-to-noise ratios with deep tissue penetration abilities. With the rationally designed probes, the NIR and TP fluorescence imaging techniques have been widely used to reveal the mechanisms of how ROS regulates and constructs complex signals and metabolic networks in TME. Therefore, we summarize the design principles and performances of NIR and TP fluorescence imaging of ROS in the TME in the last four years, as well as discuss the advantages and potentials of these works. This Review can provide guidance and prospects for future research work on TME and facilitate the development of antitumor drugs.

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A ratiometric fluorescent probe for detecting the endogenous biological signaling molecule superoxide anion and bioimaging during tumor treatment

Abstract: A fluorescent probe for O2˙− detection during tumor treatment revealed that curcumin can sensitize cisplatin for tumor treatment by inducing severe O2˙− burst.

Cited by 21 publications

References 59 publications

Novel Near-Infrared Fluorescence Probe for Bioimaging and Evaluating Superoxide Anion Fluctuations in Ferroptosis-Mediated Epilepsy

Novel Near-Infrared Fluorescence Probe for Bioimaging and Evaluating Superoxide Anion Fluctuations in Ferroptosis-Mediated Epilepsy

Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications

Deep-Tissue Fluorescence Imaging Study of Reactive Oxygen Species in a Tumor Microenvironment

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