A re-assessment of the taxonomy of Lens Mill. (Leguminosae, Papilionoideae, Vicieae)

Abstract

Three taxonomic problems exist within the gcnus Lens. Thc first is thr lark of agreement on classification and rank at the specific and sub-sprrific levels; the sccond involves tlie relationship between the recently distinguished L hn~o//ti and L odmmis or I.. &nnenfosiq and the third is the unreliability of stipulr orientation as thc primary charactcr to distinguish between L odemensic and L. n@cam. The objectives of this study were to address these problems by investigating the classification of Lmr in the light of rvidence rclating to crossability and phenetic relations, to idcntify morphological markers for taxon delimitation within the genus Lmc., with special referenre to L. lamoftti, L nigricans and I, o d e m m~, and to determine whcthcr the taxon delimitation is supported hy isozyme and RAPD rvidenre.As a result of these studies two new combinations are proposed, with I.. odmmis and I,. lammlom being rcdured to sub-species of L culinarU. A classification, key and descriptions of the accepted taxa within tlie genus are presented. 8 2000 'l'hr I.iiiiii-aii Swirly or Imidoa ADDITIONAL KEY WORDS:---classification descriptors isozynir keys lentilmorphology -RAPD.

Legume Crops Phylogeny and Genetic Diversity for Science and Breeding

“…An additional species L. odemensis was recognized by Ladizinsky et al (1984) as a new species due to the difference in stipules from L. nigricans (Ladizinsky, 1986). As the last discovered taxon, L. tomentosus was described as distinct from L. orientalis by tomentose pods, a minute satellite and one large, metacentric chromosome (Ladizinsky, 1997 (Ferguson et al, 2000). The wild relatives of the cultivated lentil have a wide distribution.…”

“…The DNA-based markers, such as RFLP (Havey and Muehlbauer, 1989;Muench et al, 1991;Rajora and Mahon, 1994), RAPD (Sharma et al, 1995;Ferguson et al, 2000;Sonnante and Pignone, 2001;Toklu et al, 2009;Tewari et al, 2012), AFLP (Sharma et al, 1996;Zavodna et al, 2000;Duran and Perez de le Vega, 2004;Kahraman et al, 2004;Rubeena et al, 2006;Fiocchetti et al, 2009;Toklu et al, 2009), microsatellite markers (Duran and Perez de le Hamwieh et al, 2009;Liu et al, 2008;Babayeva et al, 2009;Reddy et al, 2010;Gupta et al, 2012, Tewari et al, 2012Zaccardelli et al, 2012), inter simple sequence repeat (ISSR) (Zavodna et al, 2000;Sonnante and Pignone, 2001;Toklu et al, 2009), internal transcribed spacers (ITS) (Fernandez et al, 2000;Mayer and Bagga, 2002;Sonnante et al, 2003), non-transcribed spacer (NTS) (Fernandez et al, 2005), sequenced tagged microsatellite site (STMS) (Inder et al, 2008;Datta et al, 2011), single-nucleotide polymorphisms (SNPs) (Alo et al, 2011), and resistance gene analogue (RGA) (Yaish et al, 2004;Sari et al, 2013) were used to analyze phylogenetic relationships among taxa in the genus Lens, as well as the genetic diversity of cultivated lentil.…”

“…Most other national collections hold some portion of the subsets of this collection (Coyne and McGee, 2013). The ICARDA has globally mandated research for lentil improvements; it holds roughly 600 wild accessions and the largest collection of wild Lens accessions from 12 countries (Furman, 2006 (Ferguson et al, 2000;Sarker and Erskine, 2006) representing 24 countries. Ex situ collections give priority to the conservation of lentil landraces in order to maximize domestic diversity, as well as to conserve cultivars and landraces that have valuable combinations of traits and assembled linkage groups of valuable genes (Furman et al, 2009).…”

A New Method of Phenotyping and Utilization of Wild Lens Taxa for Diversification of Primary Genepool

“…and L. lamottei Czefr. [1]. Lentil is an important pulse crop of Indian sub-continent, the Middle-East, Southern Europe and Eastern and Northern Africa.…”

Genetic relationships among wild Lens Mill. species revealed by SDS-PAGE

“…is still a topic open to discussions. The classifi cation suggested by Barulina (1930) Two more species were later added to Lens genus (Oss et al 1997 Though there was an opinion that L. tomentosus and L. odemensis should be reduced to sub-species of L. culinaris (Ferguson et al 2000), data of molecular and cytological analysis (Galasso 2003, Sonnante et al 2003, Duran 2004) confi rmed isolation of L. lamottei and L. tomentosus to independent species. So, most contemporary researches recognized the next seven taxa of genus Lens:…”