A reagentless amperometric biosensor for creatinine assay based on recombinant creatinine deiminase and N-methylhydantoin-sensitive CoCu nanocomposite

Stasyuk, Nataliya; Zakalskiy, Andriy; Nogala, Wojciech; Gawinkowski, Sylwester; Ratajczyk, Tomasz; Bonarowska, Magdalena; Demkiv, Olha; Zakalska, Oksana; Gonchar, Mykhailo

doi:10.1016/j.snb.2023.134276

Cited by 4 publications

(1 citation statement)

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“…A calibration curve for creatinine determination showed on Figure . The biosensor was characterized by a minimal detection limit of 5 μM creatinine, which is better than known analogues (20 μM and 50 μM) [7,8]. Other biosensors with lower detection limit use complex and expensive modifications, such as nanoparticles [9].…”

Section: Resultsmentioning

confidence: 99%

Development of Creatinine-Sensitive Biosensor Based on Immobilized Creatinine Deiminase

Bakhmat

2024

Biotechnol. acta

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Aim.Thе purpose of the work was to develop a new construction of enzyme biosensor based on creatinine deiminase for highly sensitive creatinine determination. Methods. A new construction of enzyme biosensor based on creatinine deiminase was developed for the creatinine determination. A differential pair of gold interdigitated electrodes deposited onto a ceramic substrate was used as the electrochemical transducer. Creatinine deiminase was immobilized by cross-linking with glutaraldehyde on the surface of electrodes. Results. The biosensor showed high sensitivity towards creatinine, the limit of detection was 5 µM. The biosensor was characterized by wide linear range of creatinine determination, high reproducibility of responses and showed high storage stability – after 50 days storage the biosensor retained 83% of the initial response value. Conclusions. In future the developed biosensor can be used for express evaluation of the creatinine in biological samples.

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Section: Resultsmentioning

confidence: 99%