A Reappraisal of the Effect of Actinomycin D and Cordycepin on the Multiplication of Cowpea Mosaic Virus in Cowpea Protoplasts

Abstract

SUMMARYActinomycin D (AD) administered to cowpea mosaic virus-infected cowpea protoplasts immediately after inoculation inhibited virus multiplication, whereas late in the incubation period neither virus multiplication nor host DNA transcription were affected. The data obtained suggested that neither virus uncoating nor encapsidation were inhibited by AD. The inhibition of virus multiplication was manifested as a decrease in the level of progeny viral nucleoproteins and (+) and (-) viral RNAs. Cordycepin strongly inhibited coat protein production and (+) and (-) viral RNA synthesis throughout the incubation period.

RNA processing body (P-body) dynamics in mesophyll protoplasts re-initiating cell division

“…Treating protoplasts with ActD (20 μM) for 3 h (ActD is less permeable to plant cells than CHX e.g. De Varennes et al 1985 ) resulted in a significant reduction in the number of cells expressing P-bodies (Fig. 3 c) but only a small reduction in the number of P-bodies per cell with large variation (Fig.…”