A reappraisal of the genus <i><scp>L</scp>eptocylindrus</i> (<scp>B</scp>acillariophyta), with the addition of three species and the erection of <i><scp>T</scp>enuicylindrus</i> gen. nov

Nanjappa, Deepak; Kooistra, Wiebe H. C. F.; Zingone, Adriana

doi:10.1111/jpy.12102

Cited by 38 publications

(60 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Kooistra et al 2008), Chaetoceros (e.g. Beszteri et al 2005, Amato et al 2007, Kooistra et al 2010, Lundholm et al 2012, Asterionellopsis (Kaczmarska et al 2014) and Leptocylindrus (Nanjappa et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Temporal variation of Skeletonema community composition from a long-term time series in Narragansett Bay identified using high-throughput DNA sequencing

Canesi¹,

Rynearson²

2016

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Temporal variation of Skeletonema community composition from a long-term time series in Narragansett Bay identified using high-throughput DNA sequencing

Canesi¹,

Rynearson²

2016

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

“…(Figure 3(d)). The specificity was also tested in another species of the same family, T. belgicus, which was previously considered a synonym of Leptocylindrus minimus Meunier but was recently re-classified and placed into a newly established genus [28] (Figure 3(d)). All the differences above were statistically significant with a p value < 0.05.…”

Section: Resultsmentioning

confidence: 99%

“…Likewise, the binding capability of seq20 resulted around in sixfold higher for L. danicus with respect to P. multistriata and S. marinoi. Furthermore, we tested the binding specificity of the aptamer seq20 for L. danicus (selection) with two recently described species, L. aporus and L. convexus [28], belonging to the same family, the Leptocylindraceae. These species are defined as pseudocryptic, because they show subtle morphological differences but are genetically distinct.…”

Section: Resultsmentioning

confidence: 99%

“…[36,37] An additional challenge was the growing evidence that what it is normally classified as L. danicus is indeed a cluster of different species that co-exist in the same area showing an intriguing seasonal cycle. [28] This offered the opportunity for testing the discriminating capability of aptamers vs. congeneric, hardly distinguishable species.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Aptamers are an innovative and promising tool for phytoplankton taxonomy and biodiversity research

Santamaria

Esposito

Cerchia

et al. 2015

Chemistry and Ecology

Self Cite

View full text Add to dashboard Cite

2015) Aptamers are an innovative and promising tool for phytoplankton taxonomy and biodiversity research, Chemistry and Ecology, 31:1, 92-103, Nucleic acid aptamers are small-size ligands that selectively bind to molecular segments even when they protrude from cell surfaces. Due to their high specificity, aptamers are widely used in biomedical research and as probes for different applications. Here, we tested whether aptamers can also discriminate among phytoplankton cells. As a proof of concept we focused on the widespread centric diatom Leptocylindrus danicus and generated two aptamers that selectively bind to its cell surface. The aptamers did not bind to other diatoms tested, which included both pennate (Pseudo-nitzschia multistriata) and centric (Skeletonema marinoi, Chaetoceros socialis) species. They also showed negative binding to closely related species (Tenuicylindrus belgicus, Leptocylindrus aporus, Leptocylindrus convexus), which are hardly recognizable with microscopy techniques. In addition, aptamers discriminated also among cells of the same clone, suggesting a potential use of aptamers as clone-specific/stage-specific probes to track phytoplankton life stages in their natural environment. While the method still needs to be tested with natural algal samples, it can complement in a unique way the existing approaches to discriminate among species and possibly life stages of marine phytoplankton. The method can find useful application in taxonomic and ecological studies as well as in environmental monitoring including early warning strategies.

show abstract

“…[1]), the majority of molecular-based taxonomic revisions points at cryptic or pseudo-cryptic species, particularly in diatoms, implying that the actual diversity of these microorganisms is underestimated [2]. The utility of describing cryptic and pseudo-cryptic species and tracing their geographic and ecological ranges is demonstrated by the observations that such species can have different biochemical [3] and physiological characteristics [4], [5], which may underpin different seasonal and spatial ranges [6], [7].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Species Diversity and Distribution of an Ancient Diatom Lineage Using a DNA Metabarcoding Approach

et al. 2014

Self Cite

View full text Add to dashboard Cite

BackgroundContinuous efforts to estimate actual diversity and to trace the species distribution and ranges in the natural environments have gone in equal pace with advancements of the technologies in the study of microbial species diversity from microscopic observations to DNA-based barcoding. DNA metabarcoding based on Next Generation Sequencing (NGS) constitutes the latest advancement in these efforts. Here we use NGS data from different sites to investigate the geographic range of six species of the diatom family Leptocylindraceae and to identify possible new taxa within the family.Methodology/Principal FindingsWe analysed the V4 and V9 regions of the nuclear-encoded SSU rDNA gene region in the NGS database of the European ERA-Biodiversa project BioMarKs, collected in plankton and sediments at six coastal sites in European coastal waters, as well as environmental sequences from the NCBI database. All species known in the family Leptocylindraceae were detected in both datasets, but the much larger Illumina V9 dataset showed a higher species coverage at the various sites than the 454 V4 dataset. Sequences identical or similar to the references of Leptocylindrus aporus, L. convexus, L. danicus/hargravesii and Tenuicylindrus belgicus were found in the Mediterranean Sea, North Atlantic Ocean and Black Sea as well as at locations outside Europe. Instead, sequences identical or close to that of L. minimus were found in the North Atlantic Ocean and the Black Sea but not in the Mediterranean Sea, while sequences belonging to a yet undescribed taxon were encountered only in Oslo Fjord and Baffin Bay.Conclusions/SignificanceIdentification of Leptocylindraceae species in NGS datasets has expanded our knowledge of the species biogeographic distribution and of the overall diversity of this diatom family. Individual species appear to be widespread, but not all of them are found everywhere. Despite the sequencing depth allowed by NGS and the wide geographic area covered by this study, the diversity of this ancient diatom family appears to be low, at least at the level of the marker used in this study.

show abstract

A reappraisal of the genus Leptocylindrus (Bacillariophyta), with the addition of three species and the erection of Tenuicylindrus gen. nov

Cited by 38 publications

References 45 publications

Temporal variation of Skeletonema community composition from a long-term time series in Narragansett Bay identified using high-throughput DNA sequencing

Temporal variation of Skeletonema community composition from a long-term time series in Narragansett Bay identified using high-throughput DNA sequencing

Aptamers are an innovative and promising tool for phytoplankton taxonomy and biodiversity research

Assessment of Species Diversity and Distribution of an Ancient Diatom Lineage Using a DNA Metabarcoding Approach

Contact Info

Product

Resources

About