A recombinant fungal defensin-like peptide-P2 combats Streptococcus dysgalactiae and biofilms

Zhang, Qingjuan; Yang, Na; Mao, Ruoyu; Hao, Ya; Ma, Xuanxuan; Teng, Da; Fan, Hong-Tao; Wang, Jianhua

doi:10.1007/s00253-021-11135-y

Cited by 11 publications

(12 citation statements)

References 55 publications

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“…Finally, the slides were washed with PBS and observed by Zeiss LSM880 confocal microscope (CLSM). The excitation/emission for these dyes are 480/500 nm for SYTO 9 stain and 490/635 nm for propidium iodide ( Zhang et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…According to the previous study, the biofilm of S. dysgalactiae CVCC 3938 was analyzed by SEM with some modifications. A volume of 360 μL S. dysgalactiae CVCC 3938 bacterial suspension (1 × 10 8 CFU/mL) and 40 μL 160 × MIC NZ2114 or antibiotics were added into 24-well plates with sterile guide sheets and co-incubated at 37°C for 24 h. After incubation, the plates were washed 3 times with PBS to remove planktonic bacteria and the biofilm was fixed with 2.5% glutaraldehyde at 4°C for 24 h. The cells of biofilms were dehydrated with ethanol series (50, 70, 85, 95, and 100%) for three times with 15 min/time, dried by CO 2 , sputtered with platinum, and observed using a QUANTA200 SEM (FEI, Philips, Netherlands) ( Zhang et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…Biofilm growth kinetics measurement S. dysgalactiae CVCC 3938 is a strong biofilm-producing strain according to the previous study (Zhang et al, 2021). The growth of biofilms was measured with a 3-(4,5dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (Sigma) (Pires et al, 2011).…”

Section: Minimum Inhibitory Concentrations Determinationmentioning

confidence: 99%

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Effect of NZ2114 against Streptococcus dysgalactiae biofilms and its application in murine mastitis model

Yang

Zhang²,

Mao³

et al. 2022

Front. Microbiol.

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Bovine mastitis caused by Streptococcus dysgalactiae (S. dysgalactiae) is usually treated with antibiotics, which may potentially increase drug resistance as the abuse. NZ2114, a variant of fungal defensin plectasin, displayed a potent antibacterial activity against S. dysgalactiae. The inhibition/eradication effect of the antimicrobial peptide NZ2114 on the early/mature biofilm of S. dysgalactiae CVCC 3938 was evaluated, as well as the elimination of bacteria in mature biofilms. In this study, NZ2114 displayed potent antibacterial activity against S. dysgalactiae CVCC 3938 and three clinical isolated S. dysgalactiae strains (0.11-0.45 μM). The early biofilm inhibition of S. dysgalactiae CVCC 3938 was 55.5–85.9% after treatment with NZ2114 at concentrations of 1–16 × MIC, which was better than that of vancomycin at the same concentration. The mature biofilm eradication rate was up to 92.7–97.6% with the increasing concentration (2–16 × MIC) of NZ2114, and the eradication rate did not change significantly with further increase of NZ2114 concentration, while the biofilm eradication rate of vancomycin-treated group at the same concentration remained at 92.5%. NZ2114 reduced the number of persister bacteria in biofilm. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) further demonstrated that NZ2114 could effectively reduce the biofilm thickness and bacterial number of S. dysgalactiae CVCC 3938. In vivo therapeutic effect of NZ2114 on murine mastitis model showed that NZ2114 was better than vancomycin in alleviating mammary gland inflammation by regulating cytokines production, inhibiting bacterial proliferation, and reducing the number of mammary gland bacteria. These data suggested that NZ2114 is a potential peptide candidate for the treatment of mastitis.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Effect of NZ2114 against Streptococcus dysgalactiae biofilms and its application in murine mastitis model

Yang

Zhang²,

Mao³

et al. 2022

Front. Microbiol.

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“…The mechanism of action of P2 against bacteria varies from cytoplasmic membrane permeabilization to interaction with intracellular genomic DNA; however, the degree of damage to different bacteria is different. P2 caused Streptococcus dysgalactiae cellular content leakage and cell shrinkage, and some S. dysgalactiae cells appeared to display a “ghosting” phenomenon, but S. aureus maintained cells integrity after treatment with P2 [ 25 , 26 ]. P2 also effectively inhibited early biofilm formation, eradicated mature biofilms, and even killed persisters of S. dysgalactiae and S. aureus [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…P2 caused Streptococcus dysgalactiae cellular content leakage and cell shrinkage, and some S. dysgalactiae cells appeared to display a “ghosting” phenomenon, but S. aureus maintained cells integrity after treatment with P2 [ 25 , 26 ]. P2 also effectively inhibited early biofilm formation, eradicated mature biofilms, and even killed persisters of S. dysgalactiae and S. aureus [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Pharmacodynamics and Bactericidal Mechanism of Fungal Defensin-Derived Peptides NZX and P2 against Streptococcus agalactiae

Yang

Mao

et al. 2022

Microorganisms

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(1) Background: Based on the hazard of Streptococcus agalactiae to human and animal health and the increasing drug resistance, it is urgent to develop new antimicrobial agents with high bactericidal activity and low drug resistance against S. agalactiae. This study aims to investigate in vitro pharmacodynamics and bactericidal mechanism of fungal defensin-derived peptides NZX and P2 against S. agalactiae. (2) Methods: Minimum inhibitory concentration (MIC) and mutant prevention concentration (MPC) were determined by broth dilution method and AGAR plate dilution method. Cell membrane integrity was determined by flow cytometer. Cell morphological changes were observed by scanning electron microscope (SEM) and transmission electron microscope (TEM). (3) Results: MIC values (NZX: 0.11 μM, P2: 0.91 μM) and MPC (NZX: 1.82 μM) showed their higher antibacterial activity and stronger inhibition ability of drug resistance mutation. The bactericidal mechanism was elucidated that P2 caused S. agalactiae ACCC 61733 cells to deform, bound to the cell wall, and perturbed cell membrane, resulting in K+ leakage, membrane hyperpolarization, ATP release, and reduced cell contents. Compared with P2, NZX focuses on the cell wall, and it bound to the cell wall causing cells boundary disappearance. (4) Conclusion: NZX and P2 are promising antimicrobial agents for streptococcicosis treatment.

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Resistance response to Arenicin derivatives in Escherichia coli

Wang

Yang

Teng

et al. 2021

Appl Microbiol Biotechnol

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A recombinant fungal defensin-like peptide-P2 combats Streptococcus dysgalactiae and biofilms

Cited by 11 publications

References 55 publications

Effect of NZ2114 against Streptococcus dysgalactiae biofilms and its application in murine mastitis model

Effect of NZ2114 against Streptococcus dysgalactiae biofilms and its application in murine mastitis model

In Vitro Pharmacodynamics and Bactericidal Mechanism of Fungal Defensin-Derived Peptides NZX and P2 against Streptococcus agalactiae

Resistance response to Arenicin derivatives in Escherichia coli

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