A refined comparative map between porcine chromosome 13 and human chromosome 3

Abstract

We report here the localisation of BAIAP1 (13q24), HTR1F (13q45), PTPRG (13q23) and UBE1C (13q24) by fluorescence in situ hybridisation (FISH), and BAIAP1 (Swr2114; 21 cR; LOD = 11.03), GATA2 (Sw2448; 37 cR; LOD = 8.26), IL5RA (Swr2114; 64 cR; LOD = 3.85), LMCD1 (Sw2450; 61 cR; LOD = 4.73), MME (CP; 50 cR; LOD = 7.75), RYK (Swc22; 12 cR; LOD = 18.62) and SGU003 (Sw1876; 6 cR; LOD = 16.99) by radiation hybrid (RH) mapping to porcine chromosome 13 (SSC13). The mapping of these 10 different loci (all mapped to human chromosome 3; HSA3) not only confirms the extended conservation of synteny between HSA3 and SSC13, but also defines more precisely the regions with conserved linkage. The syntenic region of the centromeric part of SSC13 was determined by isolating porcine bacterial artificial chromosome (BAC) clones (842D4 and 1031H1) using primers amplifying porcine microsatellite markers S0219 and S0076 (mapped to this region). Sequence comparison of the BAC end sequences with the human genome sequence showed that the centromeric part of SSC13 is homologous with HSA3p24.

Chromosomal localization of nine porcine genes encoding transcription factors involved in adipogenesis

“…However, we were not able to establish the precise localization of the GATA2 gene due to the limited resolution of the FISH technique. Based on our results and results obtained by radiation hybrid mapping (Van Poucke et al, 2003), we can predict that the GATA2 gene is located in the proximal part of SSC13q31.…”

“…Based on comparative chromosome painting studies it has been shown that a large part of porcine chromosome 13 (13q11 ] q46) is homologous to human chromosome 3 and a small distal part of SSC13 is homologous to HSA21 (Goureau et al, 1996). However, comparative mapping revealed several interchromosomal differences between SSC13 and HSA3 (Van Poucke et al, 2003). Four chromosome blocks were identified in HSA3 that correspond to SSC13.…”

“…Four chromosome blocks were identified in HSA3 that correspond to SSC13. The GATA2 gene was previously mapped by radiation hybrid mapping to chromosome block 4 in SSC13 (Van Poucke et al, 2003), while the SSCq24 band, harboring the PPARG locus , was classified as block 1. Our results are in agreement with the study of Van Poucke et al (2003).…”

“…The GATA2 gene was previously mapped by radiation hybrid mapping to chromosome block 4 in SSC13 (Van Poucke et al, 2003), while the SSCq24 band, harboring the PPARG locus , was classified as block 1. Our results are in agreement with the study of Van Poucke et al (2003). However, we were not able to establish the precise localization of the GATA2 gene due to the limited resolution of the FISH technique.…”

Metastatic suppressor genes inactivated by aberrant methylation in gastric cancer

“…Gene PTPRG is a member belonging to the classic tyrosine phosphatase gene family, which includes receptor genes and non-receptor genes. PTPRG is a member of receptor gene, and is situated at 3p14 chromosome [20][21][22] . Previous researches observed methylation extinguishments of other genes that belong to the same family of PTPRG, such as PTPRN2, PTPRO, etc, in hepatic cancer [23] .…”