A Regenerative Endodontic Approach in Mature Ferret Teeth Using Rodent Preameloblast-conditioned Medium

Abstract

Background: This study evaluated the effectiveness of a regenerative endodontic approach to regenerate the pulp tissue in mature teeth of ferret. The presence of odontoblastlike cells in the newly-formed tissue of teeth treated with or without preameloblast-conditioned medium was evaluated based on morphological criteria. Materials and Methods: Twenty-four canines from six ferrets were treated. The pulp was removed, and the apical foramen was enlarged. After inducing the formation of a blood clot, a collagen sponge with or without preameloblast-conditioned medium was placed underneath the cementoenamel junction. The samples were analyzed at the eighth week of follow-up. Results: Vascularized connective tissue was observed in 50% of teeth, without differences between groups. The tissue occupied the apical third of the root canals. Odontoblast-like cells were not observed in any group. Conclusion: Revitalization of mature teeth is possible, at least in the apical third of the root canal. Further experimental research is needed to produce more reliable outcomes.

A Compilation of Study Models for Dental Pulp Regeneration

“…Ferrets have the advantage of being less expensive to house and easier to maintain and breed in the laboratory than larger animals and are typically not considered as pets [111]. Because their root apices are wide open, ferret teeth lend themselves to the study of regenerative endodontic procedures where the pulp tissue is removed and bleeding is induced in order to facilitate the formation of new tissue in the root canal [133,134]. In addition, periapical infections can predictably be induced, and ferret canines can be used to investigate irrigation and medication protocols [131].…”

Micro-Computed Tomographic Analysis of Apical Foramen Enlargement of Mature Teeth: A Cadaveric Study

“…Case reports and animal studies published in recent years describe attempts to revascularize mature teeth using a regenerative approach (Laureys et al;Paryani & Kim, 2013;Saoud et al, 2016;Xu & Li, 2018;Bucchi et al, 2019) with variable results. These studies used different methods for apical foramen enlargement, thus there are variations in working length and widening of the file, among other parameters.…”