A regulatory shortcut between the Snf1 protein kinase and RNA polymerase II holoenzyme

Kuchin, Sergei; Treich, Isabelle; Carlson, Marian

doi:10.1073/pnas.140109897

Cited by 101 publications

(121 citation statements)

References 40 publications

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“…Plasmid p35mLuc containing 35S mp-Luc was used as a control reporter, and the Ubi-SnRK1A construct was used as the effector. SnRK1A activated the CaMV35S minimal promoter fourfold (Figure 3), regardless of the presence or absence of glucose, likely due to a general chromatin-remodeling activity of SnRK1A for many promoters, as has been reported for the yeast Snf1 (Kuchin et al, 2000;Lo et al, 2005). In the absence of SnRK1A overexpression, luciferase activities were low for all constructs, except for the wild-type SRC, which was induced by glucose starvation (Figure 3).…”

Section: Snrk1amentioning

confidence: 57%

The SnRK1A Protein Kinase Plays a Key Role in Sugar Signaling during Germination and Seedling Growth of Rice

et al. 2007

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Sugars repress a-amylase expression in germinating embryos and cell cultures of rice (Oryza sativa) through a sugar response complex (SRC) in a-amylase gene promoters and its interacting transcription factor MYBS1. The Snf1 protein kinase is required for the derepression of glucose-repressible genes in yeast. In this study, we explored the role of the yeast Snf1 ortholog in rice, SnRK1, in sugar signaling and plant growth. Rice embryo transient expression assays indicated that SnRK1A and SnRK1B act upstream and relieve glucose repression of MYBS1 and aAmy3 SRC promoters. Both SnRK1s contain N-terminal kinase domains serving as activators and C-terminal regulatory domains as dominant negative regulators of SRC. The accumulation and activity of SnRK1A was regulated by sugars posttranscriptionally, and SnRK1A relieved glucose repression specifically through the TA box in SRC. A transgenic RNA interference approach indicated that SnRK1A is also necessary for the activation of MYBS1 and aAmy3 expression under glucose starvation. Two mutants of SnRK1s, snrk1a and snrk1b, were obtained, and the functions of both SnRK1s were further studied. Our studies demonstrated that SnRK1A is an important intermediate in the sugar signaling cascade, functioning upstream from the interaction between MYBS1 and aAmy3 SRC and playing a key role in regulating seed germination and seedling growth in rice.

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Section: Snrk1amentioning

confidence: 57%

The SnRK1A Protein Kinase Plays a Key Role in Sugar Signaling during Germination and Seedling Growth of Rice

et al. 2007

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“…Although Gts1p is a phosphorylated protein (25), it is unlikely that Gts1p is a phosphorylation target of Snf1 kinase, because it does not have any sequence motifs for the kinase. Rather, because Gts1p was shown to bind to Snf1 kinase by our affinity precipitation analysis, we suggest that Gts1p functions as one of transcriptional modulators for Snf1 kinase, which then functions in the RNA polymerase II holoenzyme complex (46). Alternatively, because Snf1 kinase has been recognized as a component of a histone kinase complex that works in concert with the histone acetyltransferase Gcn5 (47), it is possible that Gts1p regulates transcription by controlling histone modification via interaction with Snf1 kinase.…”

Section: Discussionmentioning

confidence: 88%

Gts1p Activates SNF1-dependent Derepression of HSP104 and TPS1 in the Stationary Phase of Yeast Growth

Yaguchi

Tsurugi

2003

Journal of Biological Chemistry

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We previously reported that the GTS1 product, Gts1p, plays an important role in the regulation of heat tolerance of yeast under glucose-limited conditions in either batch or continuous culture. Here we show that heat tolerance was decreased in GTS1-deleted and increased in GTS1-overexpressing cells under glucose-derepressed conditions during the batch culture and that the disruption of SNF1, a transcriptional activator of glucose-repressible genes, diminished this effect of GTS1. Intracellular levels of Hsp104 and trehalose, which were reportedly required for the acquisition of heat tolerance in the stationary phase of cell growth, were affected in both GTS1 mutants roughly in proportion to the gene dosage of GTS1, whereas those of other Hsps were less affected. The mRNA levels of genes for Hsp104 and trehalose-6-phosphate synthase 1 changed as a function of GTS1 gene dosage. The Q-rich domain of Gts1p fused with the DNA-binding domain of LexA activated the transcription of the reporter gene LacZ, and Gts1p lacking the Q-rich domain lost the activation activity of HSP104 and TPS1. Furthermore, Gts1p bound to subunits of Snf1 kinase, whereas it did not bind to DNA. Therefore, we suggested that GTS1 increases heat tolerance by mainly activating Snf1 kinase-dependent derepression of HSP104 and TPS1 in the stationary phase of yeast growth.We reported that the GTS1 gene shows pleiotropic effects on yeast in batch cultures, including the effect on heat tolerance as a function of gene dosage (1); overexpression of GTS1 increases and deletion of GTS1 decreases the heat tolerance of yeast in the stationary phase of growth, whereas no such effects were found in exponentially growing cells. Independently, Bossier et al. (2) isolated GTS1 from cDNA library-transformed yeast which re-grow after lethal heat shock and found that overexpression of GTS1 results in an unchanged growth rate at 37°C compared with 28°C. On the other hand, we reported that GTS1 is involved in regulating ultradian oscillations of energy metabolism in continuous cultures under aerobic and glucose-limited conditions and in the coupling of oscillations of cellular responses to various stress conditions, including heat with the energy metabolism oscillation (3-5). These results suggested that the gene product Gts1p plays an important role in the regulation of heat and other stress responses under glucose-limited or -depleted conditions in either batch or continuous culture.

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“…Snf1 regulates transcription in numerous ways, including transcription factor activation and inactivation (14 -19), modification of chromatin (20 -22), and perhaps by acting directly on the transcription apparatus (23). Snf1 also has a role in posttranscriptional regulation of gene expression.…”

mentioning

confidence: 99%

The AMP-activated Protein Kinase Snf1 Regulates Transcription Factor Binding, RNA Polymerase II Activity, and mRNA Stability of Glucose-repressed Genes in Saccharomyces cerevisiae

Young

Zhang

Shokat

et al. 2012

Journal of Biological Chemistry

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A regulatory shortcut between the Snf1 protein kinase and RNA polymerase II holoenzyme

Cited by 101 publications

References 40 publications

The SnRK1A Protein Kinase Plays a Key Role in Sugar Signaling during Germination and Seedling Growth of Rice

The SnRK1A Protein Kinase Plays a Key Role in Sugar Signaling during Germination and Seedling Growth of Rice

Gts1p Activates SNF1-dependent Derepression of HSP104 and TPS1 in the Stationary Phase of Yeast Growth

The AMP-activated Protein Kinase Snf1 Regulates Transcription Factor Binding, RNA Polymerase II Activity, and mRNA Stability of Glucose-repressed Genes in Saccharomyces cerevisiae

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