A Relation of Nucleic Acid Synthesis to Radiation-Induced Mutation Frequency in Bacteria

Haas, Felix L.; Doudney, C. O.

doi:10.1073/pnas.43.10.871

Cited by 81 publications

(16 citation statements)

References 4 publications

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“…Recombinants were selected on M minimal agar medium (Haas & Doudney, 1957) supplemented as follows: glucose, 0.4 yo (w/v); streptomycin 300 ,ug./ml. ; proline, leucine 10 ,ug./ml.…”

Section: Methodsmentioning

confidence: 99%

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Effect of -Radiation on the Donor Ability of recA and recA+ Strains of Escherichia coli

Green

Bridges

Riazuddin

1971

Journal of General Microbiology

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S U M M A R YThe sensitivity to y-radiation of the chromosome of a r e d + Hfr strain of Escherichia coli was six times greater when assayed by donor ability in conjugation than when assayed by survival in unmated bacteria. With a recA Hfr strain sensitivities were comparable when assayed by the two methods, and were similar to the sensitivity of donor ability in the r e d + strain. We suggest that the greater resistance of survival in the r e d + strain may be due to apost-replicational repair process which does not occur in the recA strain and which is prevented from occurring when a chromosome of either strain is involved in conjugation. When a recA Hfr and a recA F-strain were given 8.5 krads y-radiation, enough to inhibit completely normal DNA synthesis, the DNA synthesis associated with conjugation still occurred. This suggests that the lesions which prevent recombinant formation may act not by inhibiting chromosome transfer, but by affecting a later stage in the process of integration.

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“…Recombinants were selected on M minimal agar medium (Haas & Doudney, 1957) supplemented as follows: glucose, 0.4 yo (w/v); streptomycin 300 ,ug./ml. ; proline, leucine 10 ,ug./ml.…”

Section: Methodsmentioning

confidence: 99%

“…Smith & Meun (1970) have shown that these gaps are not filled in a u.v.-irradiated recA strain. HowardFlanders, Rupp, Wilkins & Cole (1968) suggested that the gaps in the newly synthesized DNA must be filled for the cell to survive, and that this involves recombination between the daughter c hr om0 somes .…”

Section: Introductionmentioning

confidence: 99%

Effect of -Radiation on the Donor Ability of recA and recA+ Strains of Escherichia coli

Green

Bridges

Riazuddin

1971

Journal of General Microbiology

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“…Reversions to tryptophan independence in strain WP 2 of Escherichia coli were studied in cultures grown at 37" in a minimal medium ('M' of Haas & Doudney, -1957) supplemented with 6pg. tryptophan/ml.…”

Section: Methodsmentioning

confidence: 99%

'Take-over'--an Unusual Selection Process in Steady-State Cultures of Escherichia coli

Munson

Bridges

1964

Journal of General Microbiology

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When the proportion of prototrophic revertants in a continuous culture of the tryptophan-requiring strain W P~ of Escherichia coli was adjusted to exceed about lo-', a large increase ('take-over') often occurred after a variable delay, the proportion becoming as high as 50 yo in some cases. The phenomenon appeared to be correlated with a selective advantage of revertants which became attached, with parent auxotrophs, to the wall of the culture tube. After some hours the film of growth on the wall was composed of approximately lo7 bacteria. It was quite different from the massive ' sticky ' growth sometimes encountered in continuous cultures of E. coli. The experimental observations of this ' take-over ' are quantitatively consistent with a hypothesis of wall adsorption of rather rare and readily attached variant prototrophs.

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“…The cultures were then harvested and washed in M9 minimal medium of Haas & Doudney [1957]. A 1/10 dilution of the culture was made, giving a final concentration of approximately 1-3 x lo6 viable cells/ml.…”

Section: Media and Proceduresmentioning

confidence: 99%

Effects of antimutagens on prophage induction in E coli lysogenic for lambda

Persons

Shankel

1983

Environ. mutagen

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The effects of caffeine, acriflavine, spermine, quinacrine, and adenosine on spontaneous and UV-induced prophage induction in repair-proficient and -deficient strains of lysogenic Escherichia coli were examined. Caffeine caused a coinducing effect on UV induction in KMBL 160 (repair proficient) and KMBL 163 (uvrA), an anti-inducing effect in KMBL 161 (uvrE), and had no detectable effect in KMBL 164 (uvrC). Acriflavine increased UV induction in KMBL 160 and decreased it in all three repair-deficient mutant strains. Spontaneous induction was reduced by acriflavine in all four strains. Spermine caused a decrease in UV induction in KMBL 160 and KMBL 164, while it had limited effect in KMBL 161 and KMBL 163. For spontaneous induction spermine caused an increase in KMBL 160 and KMBL 163, a decrease in KMBL 164 and no detectable effect in KMBL 161. Quinacrine had no significant effect on spontaneous induction in KMBL 160 and KMBL 164, but caused a decrease in spontaneous induction in KMBL 161 and KMBL 163. Quinacrine produced no effect on UV induction in KMBL 160; however, it decreased UV induction in the three repair-deficient strains to some extent. A significant increase in spontaneous induction in KMBL 160 was caused by adenosine, while it had little effect in the repair-deficient strains. UV induction was not affected by adenosine.

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A Relation of Nucleic Acid Synthesis to Radiation-Induced Mutation Frequency in Bacteria

Cited by 81 publications

References 4 publications

Effect of -Radiation on the Donor Ability of recA and recA+ Strains of Escherichia coli

Effect of -Radiation on the Donor Ability of recA and recA+ Strains of Escherichia coli

'Take-over'--an Unusual Selection Process in Steady-State Cultures of Escherichia coli

Effects of antimutagens on prophage induction in E coli lysogenic for lambda

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