A fixed focal length lens (FFL) camera with on-adjustable focal length is common companions for conducting aerial photography using unmanned aerial vehicles (UAVs) due to its superiority on optical quality and wider maximum aperture, lighter weight and smaller sizes. A wide-angle 35mm FFL Sony a5100 camera had been used extensively in our recent aerial photography campaign using UAV. Since this off-the-self digital camera is categorized into a non-metric one, a stability performance issue in terms of intrinsic parameters raises a considerably attention, particularly on variations of the lens principal distance and principal point’s position relative to the camera’s CCD/CMOS sensor caused by the engine and other vibrations during flight data acquisitions. A series of calibration bundle adjustment was conducted to determine variations in the principal distances and principal point coordinates before commencing, during, and after accomplishment of the flight missions. This paper demonstrates the computation of the parameters and presents the resulting parameters for three different epochs. It reveals that there are distinct discrepancies of the principal distances and principal point coordinates prior to, during, and after the mission, that peaked around 1.2mm for the principal distance, as well as around 0.4mm and 1.3mm along the x-axis and the y-axis of the principal point coordinates respectively. In contrast, the lens distortions parameters show practically no perturbations in terms of radial, decentering, and affinity distortion terms during the experiments.