A Report on the Chromatographic Assay of Urinary Pregnanediol in Pregnancy

Biochemical Tests for the Assessment of Feto-Placental Function

“…Using a chromatographic technique, found a coefficient of variation of approximately 20 % for day to day pregnanediol excretion although Coyle et al (1956) had earlier reported little day to day variation.…”

“…Early studies were bedevilled byinadequate methods for measuring pregnanediol, but the development of more specific techniques utilising alumina chromatography (Klopper et al, 1955), and gas chromatography (Podmore, 1966) following derivative formation, have enabled a more reliable assessment of the value of pregnanediol assays in obstetrics. Values for normal pregnancies have also been estimated by colorimetric assays (Coyle et al, 1956;Shearman, 1959;Klopper and Billewicz, 1963).…”

Rapid analysis of pregnanediol in pregnancy urine

“…Urinary pregnanediol excretion in late pregnancy has been used for many years as an index of placental function (Coyle, Mitchell, and Russell, 1956;Russell, Paine, Coyle, and Dewhurst, 1958;Shearman, 1959). These workers used the method of Klopper et al (1955) which can be adopted for routine laboratory use but it is expensive in chemicals, requires skilled technical handling, and is time consuming.…”