A resin slide technique to select fixed embedded cells for transmission electron microscopy

Abstract

SUMMARY A simple technique is described to resection, for electron microscopy, 2–6 μm sections that have been observed under the light microscope. The technique can be used to select cells on the basis of light microscope ultrastructure, histochemistry and autoradiography prior to electron microscopy.

Some observations on the structure, cation content and possible evolutionary status of dinoflagellate chromosomes

“…(Numbers refer to Cambridge Culture Centre references. ) Full technical details have been given in previous publications, including information on culture of material (Kearns & Sigee, 1980;Sigee & Kearns, 1981 b), preparation of ultrathin and thick (dispersed) cryosections (Kearns & Sigee, 1980), fixation and resin embedding (Kearns & Sigee, 1980;Sigee & Kearns, 1982a), resin-slide technique (Sigee, 1976), monolayer cryotechnique (Sigee & Kearns, 1982b), use of nuclease enzymes (Sigee & Kearns, 1981c), Xray micro-analysis (Kearns & Sigee, 1980;Sigee & Kearns, 1980), and autoradiographic techniques involving Ni6 (Sigee, 1982) and Ca4 (Sigee, 1983). Some information on techniques is given here in the observations section, and in the legends to the figures.…”

“…The ultrathin section used for TEMs (Figs 2, 3), was derived from the 2 pm section of Fig. 1 using a resectioning (resin-slide) technique (Sigee, 1976) which enabled a direct comparison to be made of thick and ultrathin sections, as observed by light and electron microscopy. The well-defined arrangement of electron-dense chromatin fibrils as bands of chromatin in longitudinallysectioned chromosomes (chromosome C, Fig.…”