A resource of large-scale molecular markers for monitoring Agropyron cristatum chromatin introgression in wheat background based on transcriptome sequences

Zhang, Jinpeng; Liu, Weihua; Lu, Yuqing; Liu, Qunxing; Yang, Xinming; Li, Xiuquan; Li, Lihui

doi:10.1038/s41598-017-12219-4

Cited by 28 publications

(16 citation statements)

References 42 publications

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“…Our marker-based approach of identifying disomic lines was supported by GISH results. Therefore, the use of molecular markers from the genomes of important wild relatives to track their chromosomes in wheat is a reliable method to facilitate speedy expansion of diversity in wheat germplasm 31 , 35 , 36 . This approach would incentivize wheat breeders to develop cultivars with improved adaptability to keep pace with the projected global increase in wheat demand 37 .…”

Section: Discussionmentioning

confidence: 99%

Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines

Edet

Gorafi

Cho

et al. 2018

Sci Rep

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Developing wheat–alien chromosome introgression lines to improve bread wheat’s resistance to stresses, such as drought, salinity stress and diseases, requires reliable markers to identify and characterize the alien chromatins. Leymus mollis is a wild relative of bread wheat resistant to salinity and economically important diseases of wheat, but its genome sequence and cytological markers are not available. We devised a molecular marker-assisted strategy for L. mollis chromosome identification and applied it to produce 10 wheat–L. mollis chromosome addition lines. Using 47 L. racemosus genome polymorphic PCR markers and DArTseq genotyping, we distinguished the L. mollis chromosomes and differentiated disomic and monosomic lines by progeny test. DArTseq genotyping generated 14,530 L. mollis SNP markers and the chromosome-specific SNP markers were used to determine the homoeologous groups of L. mollis chromosomes in the addition lines. To validate the marker-based results, genomic in situ hybridization was applied to confirm the presence and cytological status of L. mollis chromosomes in the lines. This study demonstrates that adequate molecular markers allow the production and characterization of wheat–alien addition lines without in situ hybridization, which saves considerable time and effort.

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Section: Discussionmentioning

confidence: 99%

Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines

Edet

Gorafi

Cho

et al. 2018

Sci Rep

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“…A series of P genome-specific STS markers were designed through EST sequences of A . cristatum transcriptome sequences [ 32 ]. Song et al [ 20 ] mapped 255 STS markers on the physical map of A .…”

Section: Discussionmentioning

confidence: 99%

“…P genome-specific markers and 6P-specific STS markers [ 30 , 32 ] were used to detect translocation lines and deletion lines. P genome-specific markers can trace the P genomic component in wheat background, which were designed through specific DNA sequences distributing the entire P genome [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

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Physical Localization of a Locus from Agropyron cristatum Conferring Resistance to Stripe Rust in Common Wheat

Zhang

Song

Zhou

et al. 2017

IJMS

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Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), one of the wild relatives of wheat, exhibits resistance to stripe rust. In this study, wheat-A. cristatum 6P disomic addition line 4844-12 also exhibited resistance to stripe rust. To identify the stripe rust resistance locus from A. cristatum 6P, ten translocation lines, five deletion lines and the BC2F2 and BC3F2 populations of two wheat-A. cristatum 6P whole-arm translocation lines were tested with a mixture of two races of Pst in two sites during 2015–2016 and 2016–2017, being genotyped with genomic in situ hybridization (GISH) and molecular markers. The result indicated that the locus conferring stripe rust resistance was located on the terminal 20% of 6P short arm’s length. Twenty-nine 6P-specific sequence-tagged-site (STS) markers mapped on the resistance locus have been acquired, which will be helpful for the fine mapping of the stripe rust resistance locus. The stripe rust-resistant translocation lines were found to carry some favorable agronomic traits, which could facilitate their use in wheat improvement. Collectively, the stripe rust resistance locus from A. cristatum 6P could be a novel resistance source and the screened stripe rust-resistant materials will be valuable for wheat disease breeding.

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“…Genomic DNA was extracted using a modified CTAB method ( Kidwell and Osborn, 1992 ; Li et al, 2017 ). A total of 82 A. cristatum chromosome 2P-specific STS markers which were developed based on the A. cristatum transcriptome sequences ( Zhang et al, 2015 , 2017 ) were mapped to 17 2P-specific bins, and the information of STS markers was described as Li et al (2017) . Of these, eight markers located on different 2P-specific bins were selected to detect the absence or presence of 2P chromosomal segments in the translocation lines and their backcross populations ( Table 4 ).…”

Section: Methodsmentioning

confidence: 99%

Physical Mapping of a Novel Locus Conferring Leaf Rust Resistance on the Long Arm of Agropyron cristatum Chromosome 2P

Jiang

Liu

et al. 2018

Front. Plant Sci.

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Wheat leaf rust is one of the most common wheat diseases worldwide and can cause up to 40% wheat yield loss. To combat the growth and spread of leaf rust disease, continual exploration and identification of new and effective resistance genes are needed. Here, we report for the first time a locus conferring leaf rust resistance located on the long arm of Agropyron cristatum chromosome 2P in Triticum aestivum–A. cristatum 2P translocation lines. This study used 50 leaf rust races, including two Chinese major dominant leaf rust races, named by THT and PHT, and other 48 different leaf rust races collected from 11 provinces, 1autonomous region and 1 municipality of China to test the resistance to T. aestivum–A. cristatum 2P chromosome translocation lines and their backcross populations, the results indicated that the novel leaf rust resistance locus was immune or nearly immune to all tested leaf rust races. Four long arm translocation lines with different breakpoints of A. cristatum chromosome 2PL and their backcross populations were tested with leaf rust race THT at the seedling and adult stages and genotyped with 2P-specific STS markers. The results showed that the novel leaf rust resistance locus of the T. aestivum–A. cristatum 2P translocation lines was located in the chromosomal bin FL 0.66–0.86 of 2PL. Therefore, T. aestivum–A. cristatum 2P chromosome translocation lines conferring leaf rust resistance locus could provide a novel disease-resistance resource for future wheat breeding programs.

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A resource of large-scale molecular markers for monitoring Agropyron cristatum chromatin introgression in wheat background based on transcriptome sequences

Cited by 28 publications

References 42 publications

Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines

Novel molecular marker-assisted strategy for production of wheat–Leymus mollis chromosome addition lines

Physical Localization of a Locus from Agropyron cristatum Conferring Resistance to Stripe Rust in Common Wheat

Physical Mapping of a Novel Locus Conferring Leaf Rust Resistance on the Long Arm of Agropyron cristatum Chromosome 2P

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