A respiratory catalyst required for the reduction of cytochrome c by cytochrome b

A comparative study of the succinic dehydrogenase-cytochrome system in heart muscle and in kidney

“…Phosphate concentration. The concentration of phosphate buffer has a marked effect on the activities of the succinic oxidase system (Keilin & Hartree, 1949;Slater, 1949b) and of the cytochrome oxidase (Quinlan-Watson& Dewey, 1948;Slater, 1949a), but has little influence on the succinic dehydrogenase (Slater, 1949b). The concentrations of phosphate buffer given in the above methods are approximately optimal for the enzyme concerned.…”

“…Similarly, it seems that there are only minor differences in the succinic oxidase systems. It is shown in another paper (Slater, 1949c), that both tissues require a factor for the reduction of cytochrome c by cytochrome b. One point of difference between heart-muscle and kidney preparations is that the endogenous cytochrome c of the kidney preparation is relatively less active than that in the heart-muscle preparation.…”

“…The nature of these haematin compounds must await further investigation. There is evidence (Slater, 1949c) that at least a part of this haematin fraction is probably concerned, like the cytochromes, in the JATER I949…”

“…This paper describes a study of the succinic oxidase system in the two tissues, using both manometric and spectroscopic methods. A preliminary account of some of these findings has appeared elsewhere (Slater, 1948).…”

Reactivation and protection of cytochrome oxidase preparations

“…All activities are referred to a fat-free dry weight (cf. Slater, 1949a). For the HA preparations the maximum activity calculated for saturating concentration of cytochrome c was found by Slater (1948) to be around Q02 = 3400 pl./mg.…”