A Review: Klebsiella pneumoniae, Klebisellaoxytoca and Biotechnology

Abstract

Biotechnology, molecular biology and genetic engineering, and bioprospecting play a crucial role in our common future, enabling industrially important microorganisms to ensure sustainable products (fuels, chemicals, pharmaceuticals, food, drug delivery systems, medical devices etc.) and new bioeconomic opportunities. Biotechnological applications are able to provide cost-effective green alternatives to conventional industrial processes, which are currently affecting the nature and biodiversity. Klebsiella species are among the well-studied microbes both in medicine field, as ones of the most resilient opportunistic pathogens, and in industry, due to their promising biochemical properties, and their potential as better hosts than other microorganisms, for i.e. in genetic manipulation. Klebsiella oxytoca and Klebsiella pneumoniae are ubiquitously found in natural environments, but also as commensals in the human gut, and associated with a high-resistance to the first-line antibiotics. However, these specific strains are continuously isolated and studied for different industrial purposes (i.e. bulk chemicals and biofuels production, medical diagnosis, nanoparticles and exopolysaccharides synthesis, plant growth promoting activities, bioremediation and biodegradation agents etc.), and scientific results regarding their biotechnological potential could generate big impact for global bioeconomy development. Recently, research in synthetic biology gained a lot of attention, and new techniques highlight ways to reprogramme these microbial cells in view of high-yield or high-quality new chemicals obtainment. Therefore, some scientific research niches are emerging in biotechnology, and unknown metabolic pathways and genes are identified and further studied, to provide alternative solutions to the global challenges.

FastANI, Mash and Dashing equally differentiate between Klebsiella species

“…The most studied, K. pneumoniae , is considered the third worldwide leading pathogen for deaths associated to resistance, after E. coli and Staphylococcus aureus ( Murray et al, 2022 ). The genus also includes other important opportunistic human pathogens, such as K. aerogenes , K. variicola and K. oxytoca ( Tomulescu et al, 2021 ). Members of the last two species also display plant-growth promoting activities such as nitrogen-fixation and production of relevant compounds with biotechnological applications ( Tomulescu et al, 2021 ).…”

“…The genus also includes other important opportunistic human pathogens, such as K. aerogenes , K. variicola and K. oxytoca ( Tomulescu et al, 2021 ). Members of the last two species also display plant-growth promoting activities such as nitrogen-fixation and production of relevant compounds with biotechnological applications ( Tomulescu et al, 2021 ). The most recently described species, K. huaxiensis , K. grimontii and K. africanensis have been recovered from cattle and human feces/urine ( Passet & Brisse, 2018 ; Hu et al, 2019 ; Rodrigues et al, 2019 ).…”

Expression of Recombinant Taq DNA Polymerase in Oligotrophic Klebsiella oxytoca: A Novel Approach to Industrial Enzyme Production

“…Biotechnology is a pivotal scienti c discipline in the upcoming century, demanding innovations beyond current methodologies to ensure sustainable biotechnological applications (Tomulescu et al 2021). Substantial advancements in this domain will derive from inventive breakthroughs and the accumulation of knowledge.…”