A Review of Approaches to Identify Enteric Bacterial Pathogens

Amani, Jafar; Mirhosseini, Seyed Ali; Fooladi, Abbas Ali Imani

doi:10.5812/jjm.17473

Cited by 17 publications

(8 citation statements)

References 76 publications

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“…It is worth noting from this that even apparently trivial modifications may have substantial consequences, and the importance of reducing assay time by an order of magnitude cannot be overstated. For the interested reader, the use of microscopy in this application has been extensively reviewed elsewhere. − …”

Section: Spectroscopic Sensorsmentioning

confidence: 99%

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

et al. 2018

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Although a fundamental understanding of the pathogenicity of most biothreat agents has been elucidated and available treatments have increased substantially over the past decades, they still represent a significant public health threat in this age of (bio)terrorism, indiscriminate warfare, pollution, climate change, unchecked population growth, and globalization. The key step to almost all prevention, protection, prophylaxis, post-exposure treatment, and mitigation of any bioagent is early detection. Here, we review available methods for detecting bioagents including pathogenic bacteria and viruses along with their toxins. An introduction placing this subject in the historical context of previous naturally occurring outbreaks and efforts to weaponize selected agents is first provided along with definitions and relevant considerations. An overview of the detection technologies that find use in this endeavor along with how they provide data or transduce signal within a sensing configuration follows. Current "gold" standards for biothreat detection/diagnostics along with a listing of relevant FDA approved in vitro diagnostic devices is then discussed to provide an overview of the current state of the art. Given the 2014 outbreak of Ebola virus in Western Africa and the recent 2016 spread of Zika virus in the Americas, discussion of what constitutes a public health emergency and how new in vitro diagnostic devices are authorized for emergency use in the U.S. are also included. The majority of the Review is then subdivided around the sensing of bacterial, viral, and toxin biothreats with each including an overview of the major agents in that class, a detailed cross-section of different sensing methods in development based on assay format or analytical technique, and some discussion of related microfluidic lab-on-a-chip/point-of-care devices. Finally, an outlook is given on how this field will develop from the perspective of the biosensing technology itself and the new emerging threats they may face.

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Section: Spectroscopic Sensorsmentioning

confidence: 99%

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

et al. 2018

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“…Pathogenic bacteria produce various types of toxins, which generate infections that can lead to many diseases and even death. For example, cholera and Shiga cause diarrheal disease, one of the main health problems in the world . These types of bacteria can be found in food, animals, and other spaces that are easy to transmit to humans.…”

Section: Introductionmentioning

confidence: 99%

Coencapsulation of Carbon Dots and Gold Nanoparticles over Escherichia coli for Bacterium Assay by Surface-Enhanced Raman Scattering

Anchustegui

Zhu

et al. 2020

ACS Appl. Bio Mater.

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Carbon dots (C-dots) is a type of fluorescent nanomaterials with excellent properties, which in combination with gold nanoparticles (AuNPs) generate structures that can be applied in various fields of science. This research developed a hybrid nanostructure between C-dots and AuNPs using a layer-by-layer (LbL) assembly to increase the surface-enhanced Raman scattering signals (SERS) for the detection of Escherichia coli. The created nanostructure was denoted as B@C-dots@AuNPs and proved by zeta potential, scanning electron microscopy, and fluorescence assays. The results showed that the introduction of AuNPs greatly enhanced the SERS signals of C-dots. The relative intensities of peaks at 2873 and 3082 cm–1 could be used for the detection of bacterium concentration with a detection limit of ca. 102 cfu/mL. In conclusion, an easy, quick, low-cost, and nontoxic technique for the detection of bacteria was developed through the coencapsulation of C-dots and AuNPs over bacteria. The findings are envisaged to facilitate the application of SERS in microbiology and related fields.

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“…In some PCR based systems screening of 4 different targets in the same sample can be performed which is known as multiplexing process. 9,37,38 Real-time quantitative PCR is a rapid methodology for sensitive and reliable quantifying of | 305…”

Section: Diagnostic Methodsmentioning

confidence: 99%

An Overview on Novel Microbial Determination Methods in Pharmaceutical and Food Quality Control

et al. 2016

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A Review of Approaches to Identify Enteric Bacterial Pathogens

Cited by 17 publications

References 76 publications

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

Coencapsulation of Carbon Dots and Gold Nanoparticles over Escherichia coli for Bacterium Assay by Surface-Enhanced Raman Scattering

An Overview on Novel Microbial Determination Methods in Pharmaceutical and Food Quality Control

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